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In a systematic review and meta-analysis, we compared allogeneic transplant outcomes after myeloablative (MAC) vs. reduced-intensity (RIC) conditioning in patients with MDS. Only two published randomized clinical trials were found, with a pooled sample size of 183 (RIC 92; MAC 91). Both studies suggested an overall survival advantage after RIC, with a pooled hazard ratio (HR) of 0.67 (95% confidence interval [CI] 0.41-1.09) for RIC vs. MAC. Relapse results were also concordant, with a pooled HR of 1.55 (95%CI 0.74-3.25) for RIC vs. MAC. Neither result was statistically significant. Comparisons for other outcomes were unremarkable. In conclusion, the evidence for the optimal conditioning intensity in MDS is weak. Post-transplant maintenance strategies and incorporation of genomic information into decision-making may improve post-transplant outcomes. Cardiovascular disease (CVD) is a leading cause of morbidity and mortality in hematopoietic cell transplantation (HCT) survivors. In these patients, risk factors such as hypertension, diabetes, obesity, and physical inactivity are important modifiers of CVD risk. However, the period in time when HCT survivors are at highest risk of developing these risk factors, and in turn CVD, coincides with a drop in engagement in survivorship care. CC-4047 nmr We examined the feasibility and acceptability of a four-week remote risk-based monitoring (blood pressure monitor, weight scale, pulse oximeter, glucometer) and management program in 18 (11 allogeneic, 7 autologous) HCT survivors at intermediate-high risk of CVD. Median age was 66 years (range 53-74 years), 67% had hypertension, 22% had diabetes, 11% were obese (body mass index ≥30 kg/m2), 56% were intermediate risk, and 44% were at high risk of CVD. Weekly compliance with the remote monitoring schedule (≥3 readings/week using all devices) ranged from 72% (week 1) to 83% (weeks 2-4). Fifteen (83%) participants generated 86 alerts that were outside the pre-determined range of normal; 63 (73%) readings normalized without intervention, and 23 (27%) required triage by the study research nurse. Nearly all participants reported that the study kept them motivated and involved in their healthcare, and >85% agreed that the study supported their healthcare goals, helped them learn and manage their health conditions, and increased their access to healthcare. These findings may set the foundation for innovative risk-based and remote interventions to reduce the burden of CVD in this growing population of patients. BACKGROUND Antimicrobial photodynamic therapy (aPDT) using chloroaluminium phthalocyanine (ClAlPc) has high oxidative power, being an alternative for the control of biofilms, especially when the photosensitizer is administered in an appropriate release vehicle. This study aimed to develop/characterize the ClAlPc encapsulated in chitosan nanoparticles (CSNPs), and evaluate its antimicrobial properties against S. mutans biofilms. METHODS CSNPs were prepared by ion gelation, and characterization studies included particle size, polydispersion index (IPd), zeta potential, accelerated stability, absorption spectrum and ClAlPc quantification. The S. mutans biofilms were formed in bovine dentin blocks at 37 °C for 48 h under microaerophilic conditions. 8 µM ClAlPc was combined with a diode laser (InGaAlP) at 660 nm and 100 J/cm2. The aPDT toxicity was verified by dark phototoxicity. The antimicrobial activity was verified by CFU/mL and biofilm was analyzed by scanning electron microscopy (SEM). The number of viable bacteria was analyzed by ANOVA and Tukey HSD tests (α = 0.05). RESULTS The characterization revealed that the ClAlPc nanoparticles were found in nanometer-scale with adequate photophysical and photochemical properties. The aPDT mediated by ClAlPc + CSNPs nanoconjugate showed a significant reduction in the viability of S. mutans (1log10 CFU/mL) compared to the negative control (PBS, p  0.05). SEM revealed change in biofilm morphology following the treatment of bacteria with aPDT ClAlPc + CSNPs. Cells were arranged as single or in shorted chains. Irregular shapes of S. mutans were found. CONCLUSION ClAlPc nanoparticles are considered stable and aPDT mediated by ClAlPc + CSNPs nanoconjugate was effective against S. mutans biofilm. V.OBJECTIVE To evaluate the clinical efficacy and safety of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) combined with a carbon dioxide laser (CO2 laser + PDT) versus CO2 laser for the treatment of low-grade vaginal intraepithelial neoplasms (vaginal LSIL). METHODS We recruited 40 patients with vaginal LSIL and persistent HR-HPV infection and divided these individuals into two groups. The CO2 laser + PDT group (20 patients) received one CO2 laser treatment and three treatments of ALA-PDT over a one-week interval. The CO2 laser group (20 patients) received up to three CO2 laser treatments. All patients were followed up at 1 month, 3 month, 6 month and 1 year. Hybrid capture HPV DNA assay and colposcopic biopsy were performed for both groups before treatment and during each of the follow-ups. Adverse effects were also assessed. RESULTS The complete remission (CR) rates were 65% (13/20) in the CO2 laser group and 85% (17/20) in the CO2 laser + PDT group (p > 0.05). HR-HPV remission rates were 25% (5/20) in the CO2 laser group and 95% (19/20) in the CO2 laser + PDT group (p  less then   0.05) at one year after treatment. In the CO2 laser group, one patient experienced severe bleeding during treatment. Twelve patients had varying degrees of adhesions and vaginal scar stenosis. One patient underwent vaginal "dead angle" after repeated CO2 laser treatment. No severe adverse events or systemic side effects were observed in the CO2 + PDT group. CONCLUSION Topical ALA-PDT combined with CO2 laser is an effective, safe, and well-tolerated treatment for vaginal LSIL and HR-HPV infections. V.BACKGROUND Optical coherence tomography angiography (OCTA) is an emerging imaging technique which shows its advantages over visualizing microcirculation with free label. However, its shortcomings in imaging depth limit its development in dermatological field. Nowadays, the newly optical clearing agent (OCA) designed for skin optical imaging demonstrates its potential. In our study, whether this OCA can improve the imaging ability of OCTA in healthy human skin and whether the combination of them is beneficial to compare the lesions and the contralateral normal skins in the patients with port wine stains (PWS) have been investigated. METHODS Five healthy volunteers and 3 PWS patients were recruited in this study. In terms of healthy people, the opisthenar area which has same structure information as facial skin was taken for investigating the OCA's ability of enhancing OCTA imaging depth on healthy human skin, besides, in order to verifying whether the exists of skin corneum interfere OCA's function, we compareTA imaging depth and contrast were significantly improved by the OCA. link2 The OCA application is a simple and efficient clinical procedure for OCTA enhancement. Moreover, it demonstrated great clinical value to compare the normal skin and the PWS lesions in the patients by the enhanced OCTA imaging. V.Hepatitis C is a chronic infectious disease, and early detection and diagnosis are key to curing it. link3 In this study, human serum Raman spectroscopy combined with a support vector machine (SVM) classification algorithm was used to identify multiple types of hepatitis C. The HCV genome is highly mutated and its nucleic acid sequence diversity is up to 30%, according to the homology of nucleotide sequences, the virus strains were divided into seven genotypes and more than 90 subtypes, there were geographical differences in the distribution of HCV of different genotypes, and hcv-1, 2 and 3 were widely prevalent in the world, the main prevalent HCV genotypes in China include 1b,2a,3a,3b and 6a. Combined with the characteristics of Urumqi, xinjiang, China as a multi-ethnic gathering area and the distribution characteristics of HCV genotypes in Urumqi, xinjiang reported in literature, HCV1, HCV2, HCV3a and HCV3b were selected as groups in this paper (Messina et al., 2015; Chen et al., 2017; Ohno et al., 1997) [1-3]. e abovementioned groups of patients, the normalized mean Raman spectra of the HCV3a patients and HCV3b patients + HCV4 patients, the difference between the average spectra of the two groups were plotted and analyzed; the attributions, similarities and differences of the main characteristic peaks from these two groups of serum Raman spectra were described. The SVM algorithm was combined with the normalized Raman spectroscopy data to identify the two groups of patient sera with 90% identification accuracy. This study shows that serum Raman spectroscopy combined with an SVM algorithm can be used for multiclass identification of hepatitis C. V.PURPOSE The aim of the present study was to establish an assessment regarding the efficacy of final disinfection protocols and sealers on the bond strength (BS) of root canal (RC) filling materials on root dentin which has already been treated with photobiomodulation (PBT) or photodynamic therapy (PBT) respectively. METHODS One-hundred and twenty root canals were included in the present study. The selected teeth were cleaned and shaped to accept the obturation. The prepared specimens were subjected to laser therapy (PBT and PDT) and were randomized into three groups (n = 20/group) on the basis of irrigation technique Group 1 distilled water (DW) + ultrasonic irrigation (UI); Group 2 17% EDTA; Group 3 0.2% Chlorhexidine digluconate (CHx) respectively. Each specific group was further divided into two subgroups (n = 10), based on endodontic sealing agent incorporated during obturation AH Plus or MTA Fillapex. The push-out test was used to gauge the BS, whereas the evaluation was done with the help of two-way ANOVA which was followed by a subsequent Tukey post-hoc test. The chi-square test was used to calculate the mode of failure in the selected specimens (α = 5%). RESULTS The final irrigation protocols of 17% EDTA and CHX helped to enhance the BS of RC filling material with either sealer agent used (p  less then  0.05), whereas, AH Plus reported an increase in the values of BS as compared to its counterpart, MTA Fillapex (p  less then  0.05). Increased values of cohesive failure were associated with all the groups, irrespective of the tested final irrigation protocols and RC sealer agents (p  less then  0.05). There was no significant difference between the laser therapies among all the groups (p  less then  0.05). CONCLUSIONS The use of 17% EDTA and CHX and sealing material AH Plus increased the BS of the obturation on radicular dentin previously subjected to either of the mentioned laser therapies. Furthermore, no significant effect on the BS was observed with the use of lasers in the study. V.Photodynamic antimicrobial chemotherapy (PACT) and photophysical properties of the photosensitizers are being actively studied as a possible alternative for antimicrobial agents. This study focuses on the application of neutral indium 5-p-carboxyphenyl-10-15-20-(tri-4-pyridyl)porphyrin and cationic indium 5-p-carboxyphenyl-10-15-20-(tris-4-methylpyridyl)-porphyrin triiodide conjugated to 6-mercapo-1-hexanol functionalized Ag/CuFe2O4 magnetic nanoparticles for photo-inactivation of S. aureus bacteria. Comparative studies were done on quaternized and unquaternized indium porphyrin complexes conjugated to Ag/CuFe2O4, where log reduction of 9.27 was obtained for quaternized conjugate and 0.83 for unquaternized conjugate. V.