Workmanmarcus7624

All the observations supported that CEACAM1 can serve as a diagnostic marker for some cancers, such as pancreatic cancer. And high CEACAM1 expression provides a better prognosis for some cancers, such as colorectal and head and neck cancers. © King Abdulaziz City for Science and Technology 2020.CKLF like MARVEL transmembrane domain containing 2 (CMTM2) plays crucial roles in spermiogenesis, skeletogenous, growth, and development through PI3K/Akt and other pathways. The purpose of this study was to explore the expression profile and variation of different spliced CMTM2 gene in Shaanbei white cashmere goats, as well as to find the relationships between a CMTM2 promoter region 14 bp genetic variant and growth traits in 1366 Shaanbei white cashmere goats. In this study, we identified alternative CMTM2 splicing and detected the effects of the spliced variants on mRNA expression levels in tissues. Meanwhile, an unreported spliced variant of CMTM2 in goat was identified using in CDS cloning and RT-PCR, namely, CMTM2-AS2. Compared with the normal transcript (CMTM2-AS1), the novel variant had the higher expression level in muscle and liver tissues, indicating that it plays an effective role in growth traits. Furthermore, a 14 bp deletion was detected within CMTM2 promoter region, and the different genotypes were significantly associated with growth traits (e.g., body length, circumference of cannon bone) in the large group of 1366 individuals in Shaanbei white cashmere goats. We found that the body length of the individuals with II (n = 571) genotype had better phenotypes than those with DD (n = 118) and ID (n = 650) genotypes. These results have direct guiding significance for goat breeding in the future and provide a new idea for studying the characteristics and functions of CMTM2 gene in goats. © King Abdulaziz City for Science and Technology 2020.In this study, we reported the genome-wide analysis of the whole sugar transporter gene family of a legume species, peanut (Arachis hypogaea L.), including the chromosome locations, gene structures, phylogeny, expression patterns, as well as comparative genomic analysis with Arabidopsis, rice, grape, and soybean. A total of 76 AhMST genes (AhMST1-76) were identified from the peanut genome and located unevenly in 20 chromosomes. Phylogeny analysis indicated that the AhMSTs can be divided into eight groups including two undefined peanut-specific groups. Transcriptional profiles revealed that many AhMST genes showed tissue-specific expression, the majority of the AhMST genes mainly expressed in sink organs and floral organ of peanut. Chromosome distribution pattern and synteny analysis strongly indicated that genome-wide segmental and tandem duplication contributed to the expansion of peanut MST genes. Four common orthologs (AhMST9, AhMST13, AhMST40, and AhMST43) between peanut and the other four species were identified by comparative genomic analysis, which might play important roles in maintaining the growth and development of plant. Furthermore, four polymorphic sites in AhMST11, AhMST13, and AhMST60 were significantly correlated with hundred pod weight (HPW) and hundred seed weight (HSW) by association analysis. In a word, these results will provide new insights for understanding the functions of AhMST family members to sugar transporting and the potential for yield improvement in peanut. © King Abdulaziz City for Science and Technology 2020.The aim of this study was to explore the effects of human adipose-derived mesenchymal stem cells (ASCs) on the growth of gastric cancer cells in vivo and vitro and its mechanism. ASCs were isolated from abandoned adipose tissues, and the surface markers were identified by flow cytometry. In vitro experiments, HGC-27 cells cultured in ASCs-conditioned medium (CM) were assigned as the experimental group, while HGC-27 cells cultured in normal medium were as the control group. MTT and colony formation assays were performed to detect cell viability and colony formatting ability, respectively. Annexin-V/PI assay, Western blot, and caspase-3 enzyme activity assay were performed to detect cells apoptosis. The isolated ASCs could be differentiated into adipocytes and osteoblasts in vitro. Flow cytometry showed that CD73 and CD105 were positively expressed in HGC-27 cells. Compared with the mice injected HGC-27 cells only, the tumor formation in mice injected both ASCs and HGC-27 cells was significantly smaller (P  less then  0.05). The colony formation ability in experimental group was 40.09% smaller than control group (P  less then  0.05) and the cell apoptosis rate in experimental group was higher than the control group (P  less then  0.05). AZD1152-HQPA mouse Furthermore, the expressions of cleaved PARP, cleaved caspase-3 proteins, and caspase-3 enzyme viability in experimental group were significantly higher than those of control group (P  less then  0.05). In conclusion, ASCs can effectively inhibit the growth of HGC-27 cells by inducing apoptosis. © King Abdulaziz City for Science and Technology 2020.In this study, fresh cattle manure was mixed with rice straw at a ratio of 101 in fresh weight and then composted in a self-built, aerated static composting box, whose dimension was 1 m × 0.8 m × 0.8 m with a volume of approximately 0.6 m3. To deal with the inconvenient and time-consuming problem of multiple stage inoculation, a single, one-time inoculation agent containing diverse microorganisms that are active at both the initial heating and thermophilic phases was developed. A total of 12 from 42 strains isolated from the none-inoculated Experiment 1 composting system were selected as microorganismal agents in Experiment 2 according to their species, prevalence and cultural temperature. 200 mL of each microorganism enrichment broth was mixed to the inoculation group at the beginning of composting. A total of 2400 mL of sterilize distilled water was added to the control group. The parameters of temperature, moisture, pH, C/N ratio, organic matter degradation, and germination index were investigated for both inoculation and control composting groups. Results showed that inoculation did not significantly shorten composting time. However, the pile temperature was increased with the maximum temperatures of 64.6 °C and 60.3 °C for the inoculation and control groups, respectively. The degradation of organic matter was accelerated (P  less then  0.05), and significantly higher GI value (P  less then  0.05) indicated that the maturity was promoted by the inoculation microorganism. This suggests that the final composting product would provide value as alternative source of nutrients for plants. Conclusively, we suggested a multiple microorganism inoculation method to increase the efficiency and promote maturity in cattle manure composting. © King Abdulaziz City for Science and Technology 2020.