Wilkinsonhensley1056

Layer-by-layer self-assembly (LBL) is an effective method to prepare potential biomaterial with multilayer coatings, and few reports have focused on the variation of oriented microstructure during LBL process. In this study, polycaprolactone (PCL) and type І collagen (COL) were electrospun to oriented nanofibrous mats, and chitosan (CS) and COL molecules were then deposited on the mats by LBL technique. Zeta potential, FT-IR analysis and XPS measurement indicated the successful fabrication and modification. Changes in surface morphology and increase in surface roughness were observed in LBL process. Additionally, LBL-structured mats exhibited improved mechanical properties with the maximal tensile strength of 35.1 ± 7.0 MPa and the best elongation of 106.0 ± 11.5 %. CCK-8 and live/dead assays illustrated that the cell viability of the mats increased more than 20 % after LBL modification. More importantly, cells seeded onto the mats showed oriented adhesion and growth along the direction of nanofiber arrangement in LBL modified mats, which provided an effective strategy for realizing the controlled growth of cells.This study describes the formation of cellulose based polyelectrolyte charge complexes on the surface of biodegradable polycaprolactone (PCL) thin films. Anionic sulphated cellulose (CS) and protonated cationic amino cellulose (AC) were used to form these complexes with a layer-by-layer coating technique. Both polyelectrolytes were analyzed by charge titration methods to elucidate their pH-value dependent protonation behavior. A quartz crystal microbalance with dissipation (QCM-D) in combination with X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to follow the growth, stability and water content of up to three AC/CS bi-layers in aqueous environment. This was combined with coagulation studies on one, two and three bilayers of AC/CS, measuring the thrombin formation rate and the total coagulation time of citrated blood plasma with QCM-D. Stable mixed charged bilayers could be prepared on PCL and significantly higher masses of AC than of CS were present in these complexes. Strong hydration due to the presence of ammonium and sulphate substituents on the backbone of cellulose led to a significant BSA repellent character of three bilayers of AC/CS coatings. The total plasma coagulation time was increased in comparison to neat PCL, indicating an anticoagulative nature of the coatings. Surprisingly, a coating solely composed of an AC layer significantly prolonged the total coagulation time on the surfaces although it did not prevent fibrinogen deposition. It is suggested that these cellulose derivative-based coatings can therefore be used to prevent unwanted BSA deposition and fibrin clot formation on PCL to foster its biomedical application.The paper presents the results of a study on the preparation of cellulose-based composite fibres (CEL) with graphene oxide addition (GO). Composite fibres (GO/CEL) were prepared via the wet spinning method from CEL solutions in 1-ethyl-3-methylimidazolium acetate (EMIMAc) that contained a nano-addition of GO dispersion in N,N-dimethylformamide (DMF). The GO contents of the composite fibres were 0, 0.21, 0.50, 0.98, and 1.97 % w w. The fibres were coagulated in two solvents distilled water and methanol. The results demonstrated that the amount of GO additive and the type of coagulant significantly impact the physicochemical, mechanical and structural properties of the CEL and GO/CEL fibres. The use of distilled water in a coagulation bath causes a degree of crystallinity of 31.0-40.8 % (WAXS) and a shift in the thermal decomposition temperature (by approximately 19 °C) towards higher temperatures (TGA). The results demonstrate improvements in the mechanical properties of the GO/CEL fibres, which were at the level of 9.43-14.18 cN/tex. In addition, the GO/CEL fibres exhibit satisfactory GO dispersion throughout their volume.Parkinson's disease (PD) develops due to oxidative stress, mitochondrial aberrations, posttranslational modification, and α-Synuclein (α-Syn) aggregation. The α-synucleinopathy is attributed to phosphorylation and aggregation of α-Syn. A strategy to degrade or reduce phosphorylated protein paves the way to develop PD therapy. Hence, the neuroprotective efficiency of PP2A (Protein phosphatase 2) activator FTY720, loaded chitosan nanoformulation has been evaluated in vitro and ex vivo experimental PD models. read more Bio-compatible chitosan-based nanocarriers have been utilized to enhance the bio-availability and neuroprotective effect of FTY720. The neuroprotective effect of characterized nanoformulation was determined by the downregulation of PD hallmark phospho-serine 129 (pSer129) α-Syn, with anti-oxidative and anti-inflammatory potentials. The neuroprotective mechanism uncovered novel physical interaction of PP2A and polycomb group of protein Enhancer of zeste homolog 2 to mediate ubiquitination and degradation of agglomerated pSer129 α-Syn. Indeed, this study establishes the neuroprotective potential of chitosan based FTY720 nanoformulations by PP2A mediated epigenetic regulation for PD prevention.We have studied the effect of chitosan sponges, produced from chitosan batches with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the adhesion, growth and differentiation of primary human osteoblasts with an aim to offer a suitable tool for guided bone regeneration. All the chitosan sponges revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was higher on sponges having a higher DDA. Higher DDA induced a more pronounced increase in alkaline phosphatase activity, osteopontin (OPN), vascular endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf related protein-1 as compared to lower DDA. Lower DDA induced the increased secretion of osteoprotegerin and SOST as compared to higher DDA. The combination of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however reduced the secretion of OPN as compared to chitosan with similar DDA but with lower Mw.