Wilhelmsenakhtar0237

The objective function was to maximize the number of covered points while minimizing the number of PZT wafers. The proposed model was solved using a genetic algorithm and was validated on circular and square sections. Sensors were spread on the circumference of the structure rather than mounting them in the form of rings or axial lines. The optimized PZT networks had high coverage that reached 99% in simulations. Notably, the optimized model improved the preliminary solution coverage by 14%. Experimental validation was performed on the circular section (pipe). The results demonstrated the proficiency of the developed model in distributing the PZT wafers on closed sections. The coverage was further evaluated by assessing if damaged areas on the pipe surface could be identified. Artificial damage was accurately located within 18 mm from the actual location. These results demonstrate that our model efficiently distributes PZT wafers on closed structures.All-optical ultrasound (AOUS) imaging, which uses light to both generate and detect ultrasound, is an emerging alternative to conventional electronic ultrasound imaging. To date, AOUS imaging has been performed using paradigms that either resulted in long acquisition times or employed bench-top imaging systems that were impractical for clinical use. In this work, we present a novel AOUS imaging paradigm where scanning optics are used to rapidly synthesise an imaging aperture. This paradigm enabled the first AOUS system with a flexible, handheld imaging probe, which represents a critical step towards clinical translation. This probe, which provides video-rate imaging and a real-time display, is demonstrated with phantoms and in vivo human tissue.Human papillomaviruses (HPVs) such as HPV16 and HPV18 can cause cancers of the cervix, anogenital and oropharyngeal sites. Continuous expression of the HPV oncoproteins E6 and E7 are essential for transformation and maintenance of cancer cells. Therefore, therapeutic targeting of E6 or E7 genes can potentially treat HPV-related cancers. Here we report that CRISPR/Cas9-based knockout of E6 or E7 can trigger cellular senescence in HPV18 immortalized HeLa cells. Specifically, E6 or E7-inactivated HeLa cells exhibited characteristic senescence markers like enlarged cell surface area, increased β-galactosidase expression and loss of lamin B1. Since E6 and E7 are bicistronic transcripts, inactivation of HPV18 E6 resulted in knockout of both E6 and E7 and increasing levels of p53/p21 and pRb/p21, respectively. Knockout of HPV18 E7 resulted in decreased E6 expression with activation of pRb/p21 pathway. Taken together, our study demonstrates cellular senescence as an alternative outcome of HPV oncogene inactivation by CRISPR/Cas9.Vertical transmission of Homalodisca vitripennis reovirus (HoVRV) from glassy-winged sharpshooter (GWSS, Homalodisca vitripennis (Germar)) females to progeny occurred in laboratory assays at frequencies too low (2.6%-15.4%) to account for HoVRV incidence (90-100%) in field populations resident in citrus. Because citrus is immune to HoVRV and no plant host is known, horizontal transmission of HoVRV from insect-to-insect was evaluated. Exposure of colony-reared, virus-free test nymphs to HoVRV-infected source adults held in the same cage for 10 days on virus-immune cowpea resulted in HoVRV transmission (13.3%-30.7%) to test nymphs. HoVRV was not transmitted when exposure was indirect and required passive movement of virions through the xylem of immune citrus seedlings. Collectively, these results demonstrate direct insect-to-insect horizontal transmission of HoVRV, providing a plausible explanation for high incidence of HoVRV in GWSS field populations in the absence of efficient vertical transmission or a plant host.

It is practical and useful to detect patients who benefit from cardiac resynchronization therapy (CRT) by electrocardiographic (ECG) methods. In this study, the predictive role of the frontal QRS-T angle and other ECG parameters was evaluated in CRT responder patients.

Seventy-seven consecutive patients with left ventricular ejection fraction (LVEF) ≤ 35%, New York Heart Association (NYHA) classes II-III, ambulatory class IV and normal sinus rhythm, who had complete left bundle branch block and were treated with CRT were included in this study. ABC294640 Patients were classified as "CRT responders" and "CRT non responders" according to their LVEF improvement. The frontal QRS-T angle was calculated as the absolute value of the difference between the QRS and T wave axes [frontal QRS-T angle = (QRS axis-T axis)].

The mean age of the patients was 64.5 ± 9.1 years, and the average follow-up was 28 (12-47) months. The post-implantation LVEF was higher in the patients CRT responders group (p < 0.001). Post-implantation frontal QRS-T angle (p = 0.003), QRS duration (p = 0.008) and cQT interval (p = 0.012) values were much shorter in the CRT responder group. Multivariable regression analyses showed that the frontal QRS-T angle and age were independent risk factors for CRT response (p = 0.009). The results of the receiver operating characteristic curve analyses (ROC) showed that the predictive optimal cut-off value of CRT response for the frontal QRS-T angle was <135 degrees (AUC 0.69, 95% CI 0.575-0.814, p = 0.004).

The narrowed frontal QRS-T angle (<135 degrees), QRS duration and cQT interval were associated with CRT response in heart failure patients. The frontal QRS-T angle can be an independent predictor of CRT response.

The narrowed frontal QRS-T angle ( less then 135 degrees), QRS duration and cQT interval were associated with CRT response in heart failure patients. The frontal QRS-T angle can be an independent predictor of CRT response.The anaerobic digestion microbiome has been puzzling us since the dawn of molecular methods for mixed microbial community analysis. Monitoring of the anaerobic digestion microbiome can either take place via a non-targeted holistic evaluation of the microbial community through fingerprinting or by targeted monitoring of selected taxa. Here, we compared four different microbial community fingerprinting methods, i.e., amplicon sequencing, metaproteomics, metabolomics and cytomics, in their ability to characterise the full-scale anaerobic digestion microbiome. Cytometric fingerprinting through cytomics reflects a, for anaerobic digestion, novel, single cell-based approach of direct microbial community fingerprinting by flow cytometry. Three different digester types, i.e., sludge digesters, digesters treating agro-industrial waste and dry anaerobic digesters, each reflected different operational parameters. The α-diversity analysis yielded inconsistent results, especially for richness, across the different methods.