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Finally, chemometrics methods, including the partial least squares discriminant analysis (PLS-DA), back propagation neural network (BPNN) and least squares support vector machines (LS-SVM) qualitative models were used for model building and discrimination results obtained through such models were compared. According to the test results, the comprehensive discrimination accuracy of wheat from different origins by the SNV-LS-SVM model reached 96.76%, Furthermore, these results demonstrated that an accurate qualitative analysis of producing area of wheat samples could be achieved by terahertz time-domain spectroscopy combined with chemometrics, which can provide a fast and accurate solution for grain security detection and origin tracing.Urgent identification of COVID-19 in infected patients is highly important nowadays. Förster or fluorescence resonance energy transfer (FRET) is a powerful and sensitive method for nanosensing applications, and quantum dots are essential materials in FRET-based nanosensors. The QDs are conjugated to DNA or RNA and used in many applications. Therefore, in the present study, novel fluorescence DNA-conjugated CdTe/ZnS quantum dots nanoprobe designed for detection of Covid-19 after extracting their RNA from saliva of hesitant people. Selleck BTK inhibitor For achieving this purpose, the water-soluble CdTe/ZnS QDs-DNA prepared via replacing the thioglycolic acid (TGA) on the surface of QDs with capture DNA (thiolated DNA) throw a ligand-exchange method. Subsequently, by adding the different concentrations of complementary (target DNA) in a mixture of quencher DNA (BHQ2-labeled DNA) and the QDs-DNA conjugates at different conditions, sandwiched hybrids were formed. The results showed that the fluorescence intensity was decreased with increasing the concentration of target DNA (as a positive control). The linear equation and regression (Y = 40.302 X + 1 and R2 = 0.98) were obtained by using the Stern-Volmer relationship. The Limit of detection (LOD) was determined 0.000823 µM. The achieved results well confirm the outcomes of the RT-PCR method in real samples.Carbon quantum dots (CDs) have become one of the most popular fluorescent materials due to their intriguing performance, which are favored by many fields. However, it is difficult to synthesize CDs with high quantum yield by the simple synthesis methods. In this paper, we fabricated CDs- silicon (SiO2) spheres composites via a versatile hydrothermal route. The prepared BCD-SiO2 composites exhibited an approximately 10-fold increase in the fluorescence intensity over that of BCDs. At the same time, the purification path was simplified by the facile separation of SiO2 spheres. The prepared BCD-SiO2 composites were used to fabricate a special sensing platform for the ultrasensitive detection of urea and urease, with detection limits of 1.67 μM and 0.002 mg/mL, respectively. Furthermore, this strategy was successfully applied to the detection of real samples. This result shows that as-prepared BCDs-SiO2 composites are promising for broad application to biological analysis.In recent years, an increasing number of new synthetic cannabinoids have appeared on the drug trade market. Many of the new synthetic cannabinoids have not previously been reported. At present, there are relatively few methods available for detecting synthetic cannabinoids and their metabolites in hair matrices. Therefore, we established a simple and fast method to simultaneously identify 29 synthetic cannabinoids and their metabolites in human hair by UPLC-MS/MS. Twenty milligrams of hair was used and processed by cryo-grinding and extraction with methanol. A Waters Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 µm) was used for chromatographic separation. Mobile phase A was comprised of 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile and water, and mobile phase B was acetonitrile. The method was fully validated and proved to have good selectivity, accuracy, precision, and satisfactory linearity within the calibrated range. The limit of detection (LOD) ranged from 0.5 to 5 pg/mg, and the lower limit of quantitation (LLOQ) ranged from 1 to 10 pg/mg. The extraction recovery was 36.1-93.3%, and the matrix effect was 19.1-110.0%. The validated method was successfully used to qualitatively and quantitatively analyze 29 synthetic cannabinoids and their metabolites in 59 actual hair samples. MDMB-4en-PINACA had the highest positive detection rate followed by ADB-BUTINACA, and there are multiple synthetic cannabinoid mixed ingestions. This methodology has great potential for the detection of 29 synthetic cannabinoids and their metabolites in forensic cases.The one-by-one examination of the increasing Open Case File (OCF) archive by comparison macroscope has become a time-consuming process and the last quarter of the 20th century witnessed the development of automated ballistic identification systems. After the utilization of these systems, government institutes, scholars and the companies devising such systems conducted various performance tests to analyze duration and correlation. The performance of BALİSTİKA and similar automated firearms identification systems are frequently measured using the pistol cartridges. This study brings a new perspective to automated ballistic identification system performance tests through using same and different brand steel 7.62 × 39 mm cartridge cases. The performance of BALİSTİKA system was measured using 4387 comparison cartridge cases according to breech face, firing pin, ejector marks and combined evaluation criteria. It was determined that correlation performance of BALİSTİKA for the same brand cartridges are higher and ejector marks are the most distinct matching criteria for 7.62 × 39 mm steel cartridge cases. The test results are presented under the headings of weapon-manufacturer countries, automated ballistic identification system matching criteria for both same and different cartridge brands.Shoe prints are commonly found at the scene of a crime and can sometimes help link a suspect to the scene. Because prints tend to be partially observed or smudgy, comparing crime scene prints with reference images from a putative shoe can be challenging. Footwear examiners rely on guidelines such as those published by SWGTREAD [1] to visually assess the similarity between two or more footwear impressions, one reason being that reliable, quantitative methods have yet to be validated for use in real cases. To help in the development of such methods, we created a study dataset of images of outsole impressions that shared class characteristics and degree of wear and that were subject to a specific type of degradation. We also propose a method to quantify the similarity between two outsole images that extends the capabilities of MC-COMP [2]. The proposed method is composed of three steps; (1) extracting image descriptors, (2) aligning images using the maximum clique, (3) calculating similarity values using two different classifiers; (a) degree of overlap between the two images, and (b) a score produced by a random forest. To explore the performance of the algorithm we propose, we compared degraded, crime scene-like images to high-quality reference images produced by the same or by different shoes. Even though comparisons involved matches or very close non-matches, and one of the images was blurry, the algorithm shows good source classification performance.Semi-packed columns are microfabricated gas chromatography columns which have a large surface area and high aspect ratio. In this paper, a new semi-packed column with high-density elliptic cylindrical posts (SCHECP) made by a micro-electro-mechanical system (MEMS) technique was reported. Compared to a semi-packed column with cylindrical posts (SCCP) under the same effective width, the surface area and aspect ratio of SCHECP were improved by 71.19% and 76.47%, respectively. To compare the performance of these two semi-packed columns, SCHECP and SCCP were fabricated. A 10-nm thick alumina film was deposited as the stationary phase by atomic layer deposition technique to ensure the uniformity and repeatability of the stationary-phase film. A contrast experiment was conducted, and the results showed that compared with SCCP, better separation performance was realized in SCHECP due to the increase in surface area and aspect ratio. The number of theoretical plates of nonane was increased by 541.84%, and the tailing factor was decreased by 54.31%.We investigated the association between perceived role overload and physical symptom experience, and the potential moderating effect of social support among caregivers of older adults. We analyzed data of 1,471 caregivers obtained from the 2017 National Study of Caregiving. In the survey, participants were assessed for physical symptoms (pain, limited leg strength, and low energy), symptom-related activity limitations, perceived role overload, and informal/formal support sources. We performed multiple logistic regression analyses with interaction terms. Caregivers who perceived higher role overload were more likely to experience the physical symptoms and frequent limited activities due to the symptoms. The associations of perceived role overload with pain and low energy, respectively, were attenuated among caregivers with high informal support. The moderating effect of formal support appeared to be the opposite in relation to pain. Caregivers who perceived high burden may benefit from their informal support to prevent detrimental effects of caregiving on physical health.Targeted radionuclide therapy (TRT) is used to treat disseminated or metastatic tumours in which conventional external beam radiotherapy (EBRT) would have unacceptable side effects. Unlike EBRT, TRT delivers low doses at a continuous low dose rate. In EBRT, the effect increases progressively with the dose rate, and biological effects (tumour control and normal tissue damage) are related to the dose according to a sigmoid curve model. This model is part of the so-called quantitative radiobiology that is mostly based on the target cell theory, according to which cell death is due to (lethal) radiation hits to vital cellular targets. This model was developed for EBRT, but was adapted to low dose-rate situations by including a parameter that reflects the time needed to repair tissue damage. However, a growing body of evidence indicates that the model should take into account also the biological effects, which are due to intercellular communications (bystander effects) and amplify the effects of radiation, as well as the immune system. Moreover, extranuclear targets must be considered, although induced intracellular and intercellular signalling pathways may ultimately result in DNA damage. It is likely that bystander effects and immune response always contribute to the overall response to TRT at different levels, and that dose and dose rate are key parameters in controlling their real contribution. We hypothesize that the dose rate is the key determinant in the balance between the physical and DNA-centred response on one side, and the biological response that integrates all subcellular compartments and intercellular signalling pathways on the other side.