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Results showed that wastewater having a high level of HCO3 and low level of NH4-N (ratio of 10201) favored only microalgal growth, and had poor P removal due to a shortage of NH4-N for PAOs growth. However, lowering the HCO3 level and increasing the NH4-N level (ratio of 10104) balanced PAOs and microalgae symbiosis, and had a positive influence on P removal. Under this mode of operation, the system was able to operate without external aeration and achieved a net P removal of 10.33 ±1.45 mg L-1 at an influent COD of 100 mg L-1. No significant variation was observed in the reactor performance for different light intensities, indicating the EBPR-PAS system can be operated at low light intensities with a positive influence on P removal.An energy-/cost-efficient and environment-friendly in-situ sludge reduction process, called the sulfidogenic oxic-settling anaerobic (SOSA) was developed recently. However, the underpinning mechanism of sludge reduction by the SOSA process remains elusive. This paper investigated the possible mechanisms of sludge reduction through biomass cultivation in three lab-scale experimental systems one anoxic-oxic CAS process with a long sludge retention time (SRT) and extended aeration (EAO) process, and two EAO-based in-situ sludge reduction processes, i.e., the conventional oxic-settling anaerobic (COSA) process and the new SOSA process. These three comparative biosystems were operated with identical influent and reactor configurations as well as the same biomass concentrations and SRTs (approximately 5 g/L and 46 days, respectively), and the sludge interchange ratios (between the CAS and side-stream reactors) in COSA and SOSA were both 10% per day. Three systems all achieved high organic (>93%) and total nitrogen (TN) (>74%) removal efficiencies. https://www.selleckchem.com/products/CP-690550.html SOSA produced 29% and 20% less sludge than EAO and COSA, respectively, simultaneously consumed 14% and 8% more oxygen than EAO and COSA, indicating that the sludge reduction in SOSA was not only caused by EAO-based aerobic digestion in the mainstream and conventional anaerobic reactions in the side-stream, but more importantly due to the bioaugmentation of sulfidogenesis. The roles of sulfidogenesis were further studied in batch tests, and the key findings were as follows i) the SOSA biomass had a faster endogenous decay rate (0.097 d-1) than that of the COSA biomass (0.045 d-1), and ii) sulfidogenesis accelerated anaerobic solubilization, hydrolysis, acidogenesis and acetogenesis by 2.3 - 3.1 times, 6 - 22 %, 22 - 60% and 6 - 22%, respectively. Overall, the mechanisms of sludge reduction in SOSA were unraveled in this study which will help promote its full-scale application in future.Water and wastewater utilities, water and sanitation hygiene (WASH) practitioners, and regulating bodies, particularly in developing nations, rely heavily on indicator microorganisms, as opposed to pathogens, for much of their regulatory decisions. This commentary illustrates the importance of considering pathogens and not relying only on indicator organisms when making decisions regarding water and sanitation, especially with respect to meeting the current targets of the Sustainable Development Goal (SDG) 6. We use quantitative microbial risk assessment (QMRA) to present three common scenarios that WASH and public health practitioners encounter to illustrate our point. These include 1) chlorination of surface water for drinking, 2) land application of latrine waste as a fertilizer, and 3) recreation/domestic use of surface waters impacted by wastewater discharge. We show that the calculated probabilities of risk of infection are statistically significantly higher when using treatment/survival information for pathogens versus using indicator species data. Thus, demonstrating that relying solely on indicators for sanitation decision making is inadequate if we truly want to achieve the SDG6 targets of safely managed water and sanitation services.Metal-based nanoparticles (NPs) can be found in wastewater streams, which are significant pathways for the release of NPs to the environment. Determination of the NPs concentration in wastewater streams is important for performing appropriate ecotoxicological evaluations. The aim of this work was to determine the incidence of NPs from 13 different elements throughout the wastewater treatment process by using single particle inductively coupled plasma mass spectrometry (spICP-MS). The incidence was determined in samples of the influent, post-primary treatment and effluent of the activated sludge process, as well as in the reclaimed water of a full-scale wastewater treatment plant (WWTP). In addition, concentration of NPs was determined in the waste activated sludge and in the anaerobic digester. The concentration of metal-based NPs in the influent wastewater were between 1,600 and 10,700 ng/L for elements such as Ti, Fe, Ce, Mg, Zn and Cu, while that for Ni, Al, Ag, Au, Co and Cd was below 100 ng/L. Concentratewater treatment train. However, anaerobic digesters store high NPs concentrations. Hence, the disposal of sludge needs to take this into account to evaluate the risk of the release of NPs to the environment.The slow electron transfer rate is the bottleneck to the biological wastewater treatment process, and the nanoparticles (NPs) has been verified as a feasible strategy to improve the biological degradation efficiency by accelerating the electron transfer. Here, we employed the Gram-positive Bacillus megaterium Y-4, capable of synthetizing Pd(0), to investigate the intra/extracellular electron transfer (IET/EET) mechanisms mediated by NPs in aerobic denitrification for the first time. Kinetic and thermodynamic results showed that the bio-Pd(0) could significantly promote the removal of both nitrate and nitrite by improving affinity and decreasing activation energy. The enzymic activity and the respiration chain inhibition experiment indicated that the bio-Pd(0) could facilitate the nitrate biotic reduction by improving the Fe-S center activity and serving as parallel H carriers to replace coenzyme Q to selectively increase the electron flux toward nitrate in IET, while promoting the nitrite reduction by abiotic catalysis.

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