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Aqueous two-phase system (ATPS) is long seen as a technique that promotes higher purity and yield in a single step. It is witnessing increased acceptance as a preferred choice for combined goals of concentration, separation and purification of a target product, be it industrially valuable or environmentally contaminating. Purification of biomolecules like enzymes, proteins, nucleic acids, viruses, etc. has been the forte of ATPS. Currently, the technique is used for concentrating the toxic fractions from diverse industrial let offs, from food, dairy, beverage, pharmaceuticals, agriculture, dyeing, tannery and metal-processing industries. Apart from being simple, efficient, rapid, flexible, economical, and biocompatible, the selectivity, purity and yield are on par and sometimes higher than the traditional downstream steps. this website From an industrial angle too, problems related to scale-up of ATPS are being actively addressed. Many novel approaches are being added by way of varying ATPS components to increase the yield and purity. Another case in point is the inclusion of optimization techniques for zeroing in on the precise setting of the operating parameters. With increasing impetus to the approach, we attempt to draw attention from academia and industries, alike, that are developing novel tweaks to the currently existing practices in ATPS. This review aims to assess and evaluate the different types of ATPS that have been used for the recovery of valuables and contaminants from industrial waste discharges.Environmental concerns due to fossil fuel usage has turned the research interest towards biomass and bioenergy field. Renewable biomass such as microalgae provides numerous advantages as they can grow in wastewater; sequester carbon dioxide, economical and eco-friendly. In this study, effect of pretreatment of microalgae (Scenedesmus obliquus) biomass using post-hydrothermal liquefaction wastewater (PHWW) for bio-oil production through hydrothermal liquefaction at a temperature of 300 °C was studied. Results showed liquefaction of pre-treated biomass yielded 48.53% bio-oil whereas 28.35% was resulted from biomass without pretreatment. The analysis of higher heating value of bio-oil showed that pretreated biomass oil has 36.19 MJ.Kg-1 against non-pretreated biomass oil, which has 28.88 MJ.Kg-1. Bio-oil (pretreated biomass) analysis revealed that 60% of compounds are in diesel and gasoline range with 58.09% of energy recovery. Bio-oil was rich in hydrocarbons of C7-C21 range with less oxygenated compounds. Carbon balance showed that an increase of 13% of carbon was sequestered in solid residue obtained from pretreated biomass and about 146% of increase also obtained in bio-oil.Studies have demonstrated that some commercial pet (i.e., cat and dog) food products contain high concentrations of mercury (Hg), and some products have Hg concentrations that are higher than expected based on the ingredients included in the package ingredient list. Additionally, concentrations of methylmercury, a particularly toxic form of Hg commonly associated with fish-based ingredients, are largely unstudied despite the widespread use of such ingredients in pet food products. This study aimed to quantify total Hg and methylmercury in a variety of commercial pet food products (n = 127), and use genetic tools to determine if specific ingredients contributed to high Hg concentrations in the final product. Results indicate that total Hg concentrations were above suggested maximum tolerable limits in three of the tested pet food products, and that methylmercury concentrations were at safe levels in all tested products. Next-generation amplicon sequencing using ten barcode primers was conducted to target distinct taxa and to determine if one primer set outperformed the others in amplifying the often heavily degraded DNA found in pet food products. The 16sUniF_16sUniR primer set generated a relatively higher number of reads across the broadest set of taxa, although several of the primer sets were useful in identifying common animal- and plant-based ingredients in commercial pet food products. Combined with the Hg results, it was demonstrated that pet food product ingredients are consistent among and between product lots. However, these results also revealed that adulteration is prevalent in pet food products.The huge excrement quantity from the increasing large-scale livestock stressed the ecological, environmental deterioration. As a major benefit for handling livestock manure, the slurry of biogas (BS) is developed during the production of biogas that might increase plant productivity. However, nitrous oxide (N2O) emissions from BS are considered a significant danger to the environment due to global warming potential. Furthermore, applying different proportions of BS combined with chemical fertilizer (CF) on N2O productions in the North China Plain (NCP) remains unclear. Herein, two sequential field trials were performed by maize-wheat rotations to substitute the CF by BS and reduce N2O emissions while keeping the crop yield stable. Four treatments were conducted, including T1, T3, T6, and CK. A total of 226.5 kg N ha-1 used in the maize-wheat rotation system. Additionally, different ratios of BS (100%, 80%, and 50%) combined with CF were used in wheat season in the tillering stage. Results showed integrated applications of BS with CF have potential for reducing N2O emission. Our findings showed that the maximum grain yield of CF was 6250 kg ha-1, which might be achieved by applying 38% BS and 62% of CF. This ratio yielded 1.03 kg ha-1 N2O emissions, which was 15% lesser than the N2O emission of CK, 1.21 kg ha-1. Considering whole growing period of wheat biogas treatments significantly reduced the cumulative N2O emissions from 17% to 26% compared to CF. To achieve maximum yield and minimum N2O emissions, an optimum 38% BS ratio has been suggested. The integrated use of BS and CF provided the greatest grain yield because of necessary nutrients provided by both slurry and CF. Consequently, N2O emissions reduced based on frequency and type of fertilizer. In conclusion, 38% ratio of BS combined with 62% CF would be a suitable approach to mitigate N2O emission and simultaneously increase crop yield in NCP.