Waddellsmed9683

001), and higher income of countries where HCWs lived (p=0.048) were significantly associated with increased anxiety.

Front-line HCWs, regardless of the levels of COVID-19 transmission in their country, are anxious when they do not feel protected. Our findings suggest that anxiety could be mitigated ensuring sufficient levels of protective personal equipment alongside greater education and information.

Front-line HCWs, regardless of the levels of COVID-19 transmission in their country, are anxious when they do not feel protected. Our findings suggest that anxiety could be mitigated ensuring sufficient levels of protective personal equipment alongside greater education and information.Various Cognitive Behavioral Therapy (CBT) programs for externalizing disorders in children and adolescents are supported by a substantial body of empirical evidence. MM3122 Most of the research evidence comes from efficacy studies conducted in university settings, but there is less knowledge about the effect of these treatments in routine clinical care. The purpose of this meta-analysis was to investigate the effectiveness of CBT in non-university settings for Attention Deficit Hyperactivity Disorder (ADHD), Conduct Disorder (CD) and Oppositional Defiant Disorder (ODD). Embase OVID, Ovid MEDLINE and PsycINFO were systematically searched for eligible studies published up to May 2020. In total, 51 treatment effectiveness studies involving 5295 patients were included. The average within-group effect size at post-treatment was significant (g = 0.91), and there were large effect sizes for both ADHD (g = 0.80) and CD/ODD (g = 0.98). At post treatment, remission rates were 38% for ADHD and 48% for CD/ODD, and the overall attrition rate was 14%. Benchmarking against efficacy studies showed that CBT in routine clinical care yields remission rates, within-group effect sizes and attrition rates that are very similar to those found in university settings. The findings support the transportability of CBT for externalizing disorders from university settings to routine clinical care. PROSPERO registration CRD42020147524.Self-renewal and multipotency are essential functions of hematopoietic stem cells (HSCs). To maintain homeostatic hematopoiesis, functionally uniform HSCs have been thought to be an ideal cell-of-origin. Recent technological advances in the field have allowed us to analyze HSCs with single cell resolution and implicate that functional heterogeneity may exist even within the highly purified HSC compartment. However, due in part to the technical limitations of analyzing extremely rare populations and our incomplete understanding of HSC biology, neither the biological meaning of why heterogeneity exists nor the precise mechanism of how heterogeneity is determined within the HSC compartment is entirely known. Here we show the first evidence that self-renewal capacity varies with the degree of replication stress dose and results in heterogeneity within the HSC compartment. Using the Hoxb5-reporter mouse line which enables us to distinguish between long-term (LT)-HSCs and short-term (ST)-HSCs, we have found that ST-HSCs quickly lose self-renewal capacity under high stress environments but can maintain self-renewal under low stress environments for long periods of time. Critically, exogeneous Hoxb5 expression confers protection against loss of self-renewal to Hoxb5-negative HSCs and can partially alter the cell fate of ST-HSCs to that of LT-HSCs. Our results demonstrate that Hoxb5 imparts functional heterogeneity in the HSC compartment by regulating self-renewal capacity. Additionally, Hoxb5-positive HSCs may exist as fail-safe system to protect from the exhaustion of HSCs throughout an organism's lifespan.Ciliogenesis is often impaired in some cancer cells, leading to acceleration of cancer phenotypes such as cell migration and proliferation. From the investigation of primary cilia of 16 gastric cancer cells (GCs), we found that GCs could be grouped into four primary cilia (PC)-positive GCs and 12 PC-negative GCs. The proliferation of the PC-positive GCs was lower than that of PC-negative GCs. To explore the role of fatty acid binding protein 4 (FABP4), which is a known oncogenic factor, in ciliogenesis, FABP4 expression and function were inhibited by transfection of cells with short interfering RNA targeting FABP4 (siFABP4) or FABP4 inhibitor treatment. Notably, the proliferation and migration of the cilia-forming GCs was effectively suppressed by inhibition of FABP4. In addition, the primary cilia in GCs were restored by a factor greater than two, suggesting a negative role of FABP4 in ciliogenesis in these GCs and FABP4 as a potential anticancer target.

Alkaline salt is one of the most devastating environmental factors limiting alfalfa productivity, however, the mechanisms underlying adaptation of alfalfa to alkaline remain unclear. Our aim is to investigate proteomic and metabolomic differences in growth and root of alfalfa under alkaline salt in Rhizobium-alfalfa symbiotic relationships.

Rhizobium-inoculated and non-inoculated alfalfa plants were treated with 200mmol/L NaHCO

to investigate physiological, metabolic, and proteomic responses of root-nodule symbiosis under alkaline-induced stress, using an integrated approach combining metabolome and proteome analysis with measurements of physiological parameters.

The improved tolerance to alkalinity was observed in RI-plants compared with NI-plants. RI-plants accumulated more proline and MDH, and had higher antioxidant activity and relatively high RWC but low MDA content and low Na

/K

ratio. The stress-related genes (P5CS, GST13, H

-Ppase, NADP-Me, SDH, and CS) were actively upregulated in RI plants under alkaline stress. In RI-plants, damage caused by alkaline stress was mainly alleviated by decreasing oxidative damage, enhancing the organic acid and amino acid metabolic processes, and scavenging harmful ROS by activating the phenylpropanoid biosynthetic pathway.

We revealed distinct proteins and metabolites related to alkali tolerance in RI-plants compared to NI-plants. Alkali tolerance of rhizobia-inoculated alfalfa was enhanced by altered proteins and metabolic processes as well as decreased oxidative damage.

We revealed distinct proteins and metabolites related to alkali tolerance in RI-plants compared to NI-plants. Alkali tolerance of rhizobia-inoculated alfalfa was enhanced by altered proteins and metabolic processes as well as decreased oxidative damage.