Underwoodviborg6392

Viral infectious diseases are serious threats to human health in both developing and developed countries. Although there is the continued development of new drugs from synthetic sources as antiviral agents, medicinal plants continue to provide the basic raw materials for some of the most important antiviral drugs. Alkaloids are a class of pharmacologically active plant compounds that are usually alkaline in nature. In this review, we tried to summarize recent progress in herb-based antiviral research, the advantages of using active plant compounds as antiviral agents, and the inflammatory responses initiated by alkaloids, based on the literature from 2009 to 2019, for the treatment of conditions, including influenza, human immunodeficiency virus, herpes simplex virus, hepatitis, and coxsackievirus infections. Articles are retrieved from PubMed, Google Scholar, and Web of Science using relevant keywords. In particular, the alkaloids from medicinal plants responsible for the molecular mechanisms of anti-inflammatory actions are identified and discussed. This review can provide a theoretical basis and approaches for using various alkaloids as antiviral treatments. More research is needed to develop alkaloidal compounds as antiviral therapeutic agents and potential regulators of the anti-inflammatory response.

Chlorogenic acid (CGA) is a type of polyphenolic substance that is widely extracted from many traditional Chinese medicines (eg,

Thunb,

Oliver) and exhibits a wide range of anti-tumor effects. However, the potential molecular mechanisms of CGA in glioma U373 cells remain unclear.

Network pharmacology analysis was used to explore the potential therapeutic targets of CGA in glioma. Human glioma U373 cells were treated with different concentrations of CGA for 24 h. CCK-8 assays were used to detect the inhibitory rate of cell growth. Annexin V-FITC/PI staining and Hoechst 33342 staining were used to detect apoptosis. PI staining was used to investigate cell-cycle progression. Wound healing assays and transwell assays were used to detect the cell migration and invasion, respectively. Western blotting and immunohistochemistry were used to measure protein levels in vitro and in vivo.

The proliferation of U373 cells was significantly inhibited by CGA in a dose- and time-dependent manner. CGA significantly arrested the cell cycle of U373 cells in the G2/M phase and induced apoptosis. Moreover, CGA significantly suppressed the migration and invasion of U373 cells. Additionally, we found that CGA inhibited the growth of U373 cells in vivo. Furthermore, network pharmacology analysis suggested that the anti-tumor effects of CGA on U373 cells were associated with the down-regulation of the SRC/MAPKs signaling pathway.

The present study indicated that CGA had anti-glioma effects on U373 cells by down-regulating SRC/MAPKs signal pathway.

The present study indicated that CGA had anti-glioma effects on U373 cells by down-regulating SRC/MAPKs signal pathway.

To compare the agreement between commercially available table mounted and a hand-held autorefractors and their agreement with subjective refraction. GSK2830371 order The effect of different body position with the handheld autorefractometer was also evaluated.

A prospective study was performed on 253 healthy eyes. Refraction was acquired by a table-mounted Huvitz and hand-held Nidek autorefractometer, subjective refraction was acquired. Refractive errors were compared in terms of spherical equivalent (SE), cylinder power, and the J0 and J45. The level of agreement was evaluated by Bland-Altman plots.

There was a significant difference in SE measurements between both devices and between them and subjective refraction (P=0.00). The Huvitz SE readings tended to be less myopic. However, limits of agreement (LOA) for SE were narrowest for Nidek sitting vs supine followed by Huvitz vs subjective SE refraction. The LOA for SE for Nidek sitting vs subjective SE were of wider range. For cylinder values, LOA were similar for all dNidek hand-held autorefractor. A highly reliable spectacle prescription could be done based on Huvitz readings. Both devices and positions could be used interchangeably in estimation of K-readings.

To find a reliable method to determine the wearing times of glasses in adults objectively on the basis of temperature measurements of the small-sized theramon

thermosensor device (TM) from orthodontics. Furthermore, the reliability of the determined wearing times during well and poor position of spectacles was explored. To address the previously reported lack of accuracy during higher outside temperatures, this study was conducted in midsummer and autumn.

Twenty adults wore their spectacles with three TMs attached to both inner sides and the right outer side in a period of three consecutive days. They were asked to report wearing and non-wearing times, indoor and outdoor activities and subjective perception of spectacle position as accurately as possible. To find the most reliable way to determine spectacle wearing times compared to protocolled wearing times, we applied and evaluated temperature categorizing methods and visual analysis of time logged temperatures. Percent error and percent accuracy of e time logged temperatures. The findings of this study require further studies on larger groups of affected, amblyopic patients and children, to analyse the real impact of spectacle wearing times and poor spectacle position on visual development.

A reliable and objective determination of spectacle wearing times is possible on the basis of TM temperature measurements in a standard clinical setting. Measurements of one inner TM seem to be sufficient to determine the wearing times and are more appealing. Poor spectacle position can be determined reliably by analysing visually the time logged temperatures. The findings of this study require further studies on larger groups of affected, amblyopic patients and children, to analyse the real impact of spectacle wearing times and poor spectacle position on visual development.

In order to better understand cataract development, we analyzed the glycosylation profile of human lens epithelial cells (HLECs) from anterior lens capsules of type 2 diabetes mellitus (T2DM) and non-diabetic (ND) patients undergoing routine cataract surgery.

Research Department of the Asociación para Evitar la Ceguera, Hospital "Dr. Luis Sánchez Bulnes", Mexico.

Experimental study.

Evaluation of anterior lens capsules from T2DM and ND patients undergoing phacoemulsification and free from other ocular diseases.

Hematoxylin-eosin staining revealed HLECs alterations in T2DM samples. From lectins with different sugar specificities used, concanavalin A showed significant differences, labeling homogeneously both in the cytoplasm and in cell membranes in ND capsules, while in T2DM capsules, in addition to membrane and cytoplasm labeling, there were perinuclear vesicles with high concanavalin A labeling. Two-dimensional gel electrophoresis showed that T2DM patients have a ~65-kDa spot with an isoelectric point of 5.