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of amino acid, low fat, a certain amount of vitamin B_2 and E, rich in minerals.

Five main products of freshwater shrimp in Anhui belong to super nutritional value food which contained high protein, variety of amino acid, low fat, a certain amount of vitamin B_2 and E, rich in minerals.

a novel and rapid analytical method was established for the extraction of N-nitrosodimethylamine in large-volume water samples.

Pretreatment was on the basis of ultrasonic-assisted-dispersed solid phase extraction. see more Coco housing activated carbon was incorporated into the water samples, and the rapid enrichment of N-nitrosodimethylamine was realized by implementing ultrasonic-assisted dispersion. After that, the suspension was filtered by suction, and the residue was eluted by dichloromethane. The eluate was collected and then concentrated to dry using rotary evaporation. Ultra-high performance liquid chromatography tandem mass spectrometer, which equipped with atmospheric pressure chemical ionization source was employed for determination under the multiple reaction monitoring mode.

Limit of detection and quantification for N-nitrosodimethylamine in 500 mL water was 2. 0 ng/mL and 6. 0 ng/mL, respectively. Throughout the validation, good linearity with R~2>0. 999 were achieved with the range from 0. 5 ng/mL to 150. 0 ng/mL. Satisfactory recovery(95. 7%-100. 8%) and inter-day reproducibility(<4. 2%) were obtained by three-level spiking experiments in different water matrices. Finally, the established method was applied for analysis of 48 real samples, the detectable concentrations of positive samples were in the range of <2. 0 ng/L to 14. 4 ng/L.

Compared with the traditional solid-phase extraction method, this method ology showed the characteristics of rapid and more efficient, and could save at least 50% of the pretreatment time. Meanwhile, the result of method ology validation were satisfactory.

Compared with the traditional solid-phase extraction method, this method ology showed the characteristics of rapid and more efficient, and could save at least 50% of the pretreatment time. Meanwhile, the result of method ology validation were satisfactory.

To investigate the damage of procymidone to penile, testis and sperm in adolescent male mice.

4-week-old male ICR mice were allocated randomly to the treatment groups and the control group, with 8 mice in each group. Procymidone was administered to mice by gavage with dose 50 mg/kg(low dose), 100 mg/kg and 200 mg/kg, while the control group was given only an equal volume of soybean oil. On the 11 th days, the mice were sacrificed by spinal dislocation, their body masses were weighed, and the anogenital distance(AGD), testicular volume and penis length were measured. Furthermore the weight of the testes, epididymis and penis were weighed, the organ coefficients were calculated, and then a testis on one side and 1/2 penis tissue were fixed in 4% paraformaldehyde and used for histology analysis. The testis on the other side and the remaining 1/2 penis were used to detect androgen content and Caspase-3, Caspase-9, Caspase-12 and Bax gene expression. At the same time, one epididymis was randomly selected to deestosterone in the testis of the middle and high dose groups were high than the control(P<0. 001), while there was no statistically significant difference of the testosterone among all the groups in the penis(P>0. 05). In addition, the expression of pro-apoptotic genes Caspase-3, Caspase-9, Caspase-12 and Bax in testis tissues of the high-dose group were significantly increased(P<0. 05).

Procymidone can cause damage to the structure and function of testes, reduce sperm quality, and increase the expression of certain pro-apoptotic genes in adolescent male mice.

Procymidone can cause damage to the structure and function of testes, reduce sperm quality, and increase the expression of certain pro-apoptotic genes in adolescent male mice.

To investigate the distribution and expression of nuclear transcription factor(NF-κB) and macrophage migration inhibitory factor(MIF) in the lung tissue of rats exposed to silica.

Atotal of sixty-four adult rats aged 10 to 12 weeks(cleaning grade) were randomly divided into control group and silica dust group(32 rats each). At day 3, 7, 14, and 28 after modeling, 8 rats were randomly sacrificed in each group. Immunohistochemistry was used to detect the distribution and expression of NF-B and MIF in lung tissue.

The positive result of NF-κB p65 were localized in the nucleus and cytoplasm, and were mainly expressed in bronchial wall epithelial cells, macrophages, neutrophils, lung mesenchymal cells, and alveolar epithelial cells. The IOD value of NF-κB p65 in the dust-exposed group was compared with the control group at each time point, and the difference was statistically significant(P<0. 05). The result of MIF immunohistochemistry in rat lung tissue showed that MIF-positive cells were mainly alveolar epithelial cells, mesenchymal macrophages and alveolar macrophages. Among them, positive expression was also found in part of the capillary endothelium, and in smooth muscle cells of the vessel wall There is also a small amount of expression. The MIF IOD value of the dust-exposed group was compared with the control group at each time point, and the difference was statistically significant(P<0. 05).

Both NF-κB and MIF are positively expressed in the lung tissue of silica-exposed rats, which has certain reference significance in the early diagnosis and treatment of silicosis in the future.

Both NF-κB and MIF are positively expressed in the lung tissue of silica-exposed rats, which has certain reference significance in the early diagnosis and treatment of silicosis in the future.

To study the safety of eight nucleic acid dyes EB, GelGreen, GoldView, GeneGreen, SYBRGreen I, GelRed, SYBRSafe and SYBRGold.

Salmonell atyphimurium TA97, TA98, TA100 and TA102 involved in the minimum inhibitory concentration(MIC) of the 8 nucleic acid dyes by the Broth dilution, which follows Clinical and Laboratory Standards Institute. The experiment tests the reverse mutation of bacteria with the 8 nucleic acid dyes, which follows GB 15193. 4-2014.

EB and its metabolites show significant mutagenicity on TA97, TA98, TA100 and TA102. SYBRGold and its metabolites do not show mutagenicity on TA97, TA98, TA100 and TA102. The other six dyes show varying mutagenicity on TA97, TA98 and TA102, meanwhile show no mutagenicity on TA100.

This research shows that, except EB, the other dyes show no mutagenicity under working concentration, but show varying mutagenicity under high concentration. SYBRSafe and SYBRGreenI are highly toxic, and the operators must have high precautions when making diluted solution of them.

This research shows that, except EB, the other dyes show no mutagenicity under working concentration, but show varying mutagenicity under high concentration. SYBRSafe and SYBRGreenI are highly toxic, and the operators must have high precautions when making diluted solution of them.

To investigate the effect of resveratrol(Res) combined with soy isoflavones(SIF) on apoptosisinduced by oxidative stress in hippocampus in aging model rats.

Sixty female SD rats were randomly divided into the Sham control group, aging model group, Res treatment group, SIF treatment group, Res combined with SIF treatment group and estrogen replacement therapy group(ERT group). Rats with aging were induced by bilateral ovariectomy combined with intraperitoneal injection of D-galactose. TUNEL and transmission electron microscopy were used to observe apoptosis and ultrastructural changes of mitochondrion in hippocampus, respectively. The activities of superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase(GSH-Px) and content of Malonaldehyde(MDA) were detected in the hippocampal homogenate. The protein expressions of cytochrome C oxidase(COX) Ⅰ, Bcl-2 associated X protein(Bax)、B-cell leukemia/lymphoma 2(Bcl-2) and cytochrome C were detected by Western blot.

Compared with the Sham control group, thratio of Bcl-2 and Bax, and inhibiting the release of cytochrome C may be the mechanisms by which Res and SIF improve apoptosis in aging rats.

To investigate the relationship between vitamin D binding protein gene rs2282679 A/C polymorphism with blood vitamin D levels.

A total of 286 eligible subjects were selected from one university in Hebei Province. Serum 25-hydroxylated vitamin D levels were measured by liquid chromatography-mass spectrometry, and gene chip was used for genotyping of rs2282679 locus. Statistical analysis was performed using R software.

A total of 285 participants in the study completed the experiment, and result indicated that the distribution of the rs2282679 locus A/C polymorphism in the participants was consistent with the Hardy-Weinberg equilibrium. The genotype of rs2282679 was significantly associated with serum vitamin D insufficiency and deficiency(P=0. 031). Allele A was a risk factor for vitamin D insufficiency and deficiency. The OR value of allele C relative to A was 0. 65, and the genetic pattern of allele C relative to A was dominant(P=0. 03).

The vitamin D-binding protein gene rs2282679 was significantly associated with serum vitamin D insufficiency and deficiency in college students, and A-allele is a risk factor accounting for vitamin D insufficiency and deficiency in college students.

The vitamin D-binding protein gene rs2282679 was significantly associated with serum vitamin D insufficiency and deficiency in college students, and A-allele is a risk factor accounting for vitamin D insufficiency and deficiency in college students.

To determine the concentration of fluctuating gaseous formaldehyde in an anatomy laboratory and to investigate its effects on the subjective symptoms and the lung functions of medical students working there.

To measure the levels of formaldehyde gas at different physical ventilation times at the center of the study laboratory by the gaseous formaldehyde detector. A total of 251 of second-year medical students were randomly selected from 1004 students participating in the anatomy operation as the survey subjects, 77 medical students were randomly selected for lung function assessment. The subjective symptoms of the medical students exposed to this gas were investigated via questionnaire surveys before, during and after the dissection operation. The probable lung function damages caused by the exposure before and after the operation were also measured using various parameters.

The concentration of gaseous formaldehyde decreased with an increase in the ventilation time(P<0. 05), but it was always hiExisting physical ventilation facilities are difficult to reduce the concentration of gaseous formaldehyde below the safety threshold. Exposure to ultra-high concentrations of gaseous formaldehyde caused a significant increase in subjective symptoms of medical students with secondary pulmonary function impairment.

Existing physical ventilation facilities are difficult to reduce the concentration of gaseous formaldehyde below the safety threshold. Exposure to ultra-high concentrations of gaseous formaldehyde caused a significant increase in subjective symptoms of medical students with secondary pulmonary function impairment.