Timmflores0446

5-FU enhanced TRAIL-induced gastric cancer cell apoptosis by inactivating the MAPK pathway. Overall, our analysis firstly provided new insights into the role of 5-FU in increasing sensitivity to TRAIL. 5-FU can be used as a sensitizer for TRAIL, and its administration is a potential strategy for the treatment of gastric cancer.In an effort to regulate waste electrical and electronic equipment (WEEE) recycling, the Chinese government has issued China-WEEE regulations and a fund policy. In accordance with the 2012 fund policy, the government collects funds from producers and subsidizes recycling enterprises. However, this policy has encountered difficulties. The 2016 White Paper on WEEE Recycling, Treatment and Reuse states that while the funds collected from producers were 2.61 billion Yuan, the subsidies provided to recycling enterprises were 4.714 billion Yuan, which is a gap of 2.104 billion Yuan. In addition, this policy did not incentivize producers to invest in WEEE recycling. Our paper aims to answering the following questions (1) What is the impact of the current WEEE processing fund policy? (2) How can producers' green innovation in WEEE recycling be promoted? We use evolutionary game theory to investigate the strategies used by producers and recyclers and explore their evolutionary behaviors under two policies the 2012 fund policy (the current policy) and the Fund Relief Policy (a newly designed policy). The results indicate that the Fund Relief Policy can motivate producers' green innovation behavior, improve the profitability of recyclers, and alleviate the financial pressure on government to a certain extent.Biodiesel production calls for innovative solutions to turn into a competitive process with a reduced environmental impact. One of the process bottlenecks stands in the immiscibility of oil and alcohol as raw materials, so mixing process largely impacts the overall process cost. This process step, if carried out by using hydrodynamic cavitation, has the possibility to become a benchmark for large scale applications. In this paper a process analysis of biodiesel production scheme is developed starting from two different feedstocks, virgin oil and waste cooking oil. At the first the traditional process scheme has been simulated, in a second simulation, the reactor for the biodiesel production is interchanged with a hydrodynamic cavitation reactor. In the paper, the comparison between the traditional and innovative process by using life cycle costing approach has been presented, thus providing indications for industrial technological implementation coming from a professional tool for process analysis. It is worth noting that the introduction of hydrodynamic cavitation reduces of about 40% the energy consumption with respect to the traditional process. As regards the total treatment costs, when using virgin oil as feedstock, they were in the range 820-830 €/t (innovative and traditional process, respectively); while starting from waste cooking oil the costs decreased of about 60%, down to 290-300 €/t (innovative and traditional process, respectively).The Italian financing system for waste collection and disposal services, still far from a price-based system at the national level, is similar to a real property tax system. Among other concerns, this raises equity issues. The aim of this paper is threefold. First, the structure of the waste tax in its components (i.e., fixed and variable) is analysed at the provincial level. This allows to investigate the magnitude of the two tax components in Italian provinces, while showing the differences among macro-areas in the financing system for waste collection and disposal services. Second, the regressivity (progressivity) of the tax system adopted is investigated, so as to highlight the differences among geographical areas. Third, an alternative base for the waste tax is proposed, in order to avoid regressivity and achieve higher equality. Metabolism inhibitor For these purposes, official data referring to 2017 are used and a Geographically Weighted Regression is implemented. The results highlight the importance of an alternative base of the waste tax to design an equal and non-regressive tax, more similar to a Pay-As-You-Throw scheme.Curcumin, a pleiotropic signalling molecule from Curcuma longa, is reported to be effective against multiple cancers. Despite its promising effect, curcumin had failed in clinical trials due to its low aqueous solubility, stability and poor bioavailability. While several approaches are being attempted to overcome the limitations, the improved solubility observed with curcumin-derived carbon dots appeared to be a strategy worth exploring. To assess if the carbon dots possess bio-activity similar to curcumin, we synthesized carbon dots (CurCD) from curcumin and ethylenediamine. Unlike curcumin, the as-synthesized curcumin carbon dots exhibited excellent solubility, excitation-dependent emission and photostability. The anti-cancer activity evaluated with glioblastoma cells using the well-established in vitro models indicated its comparable/enhanced activity over curcumin. Besides, the selective affinity of CurCD to the actin filament, indicated it's prospective to serve as a marker of actin filaments. In addition, the non-toxic effects observed in normal cells and fish embryos indicated CurCD was more biocompatible than curcumin. While this work reveals the superior properties of CurCD over curcumin, it provides a new approach to explore other plant derived molecules with similar limitations like curcumin.We propose a colorimetric sensing array consisting of 4-aminothiophenol (p-ATP)-modified gold nanoparticles (Au NPs), silver nanoparticles (Ag NPs), and core-shell Au@Ag nanocubes (Au@Ag NCs) as sensing elements to identify multiple proteins according to the diverse colorimetric response patterns. In the absence of proteins, the sensor element solution itself did not agglomerate. link2 After interacting with six proteins (lysozyme (LZM), hemoglobin (HGB), peroxidase from horseradish (HRP), bovine liver from peroxidase (CAT), trypsin from bovin pancreas (TRY), and pepsin (PEP)), due to the different binding ability between the sensing elements and various proteins, the sensing array exhibits a unique pattern of colorimetric variations, linear discrimination analysis (LDA) was applied to analyze the pattern and produced a clustering map for a clearer differentiation of these proteins.In order to maximize the therapeutic effect and and minimize the systemtic side effect of the small molecule anticancer drugs, biodegradable drug delivery systems (DDSs) that respond to tumor microenvironment (TME) have attracted significant attention. Herein, a novel redox/pH dual-responsive and biodegradable polyphosphazene (PPZ) nano-prodrugs have been prepared via one-pot crosslinking of vanillin modified DOX (VMD, acid-sensitive) and 4,4'-dihydroxydiphenyl disulfide (HPS, GSH-responsive) with hexachlorocyclotriphosphazene (HCCP). The phenol groups of the as-synthesized VMD and HPS have high nucleophilic substitution activity towards HCCP under base catalyst and afforded PPZ nano-prodrugs, denoted as HCCP-VMD-HPS, with a high drug loading ratio of up to 56.4 %. As expected, the skeleton of the PPZ consisting of imine bonds in VMD and the disulfide bonds in HPS and cyclotriphosphazenes inclined to be decomposed in low pH conditions and high level of GSH environments. The antitumor drug DOX was found to be controlled released in TME conditions (extracellular, pH∼6.8 and endosomes, lysosomes pH∼5.0 with ∼10 mM GSH), rather than neutral physiological conditions (pH 7.4 with ∼20 μM GSH). Moreover, the resulting HCCP-VMD-HPS nano-prodrug have obvious cytotoxicity to cancer cells while a negligible side effect to normal cells. We therefore believe that the prepared redox/pH dual-responsive and biodegradable PPZ DDSs have great potential in various field.In the present study, an effort has been made to understand the interaction mode of propolis, a natural substance produced by honey bees, with gram-positive and gram-negative bacterial cells by measuring alterations in cell surface physico-chemical properties following the incubation of the cells with different sub-inhibitory concentrations of this antimicrobial agent. Electrophoretic mobility and surface hydrophobicity measurements revealed for the first time that propolis induced substantial changes in the volumetric charge density, electrophoretic softness and degree of hydrophobicity characterizing the outermost surface layer of cells. These changes, which appear to be dose-dependent, seem to be consistent with the increasing accumulation and penetration of the propolis antimicrobial components through the cells extracellular layer. Moreover, electron microscopy observation and the determination of the cell constituents' release demonstrated that propolis at sub-bactericidal concentrations already provoked (at least localized) cell wall damage and/or perturbations. link3 These findings thus suggest that the initial mechanism of action of propolis is most likely structural, resulting from sufficient interaction between the different propolis components and bacterial cell wall structures.

Research on prenatal cannabis use and adverse infant outcomes is inconsistent, and findings vary by frequency of use or cigarette use. We assess (1) the prevalence of high frequency (≥once/week), low frequency (<once/week), and any cannabis use during pregnancy by maternal characteristics and adverse infant outcomes; (2) the prevalence of infant outcomes by cannabis use frequency, stratified by cigarette smoking; and (3) the association between cannabis use frequency and infant outcomes, stratified by cigarette smoking.

Cross-sectional data from 8 states' 2017 Pregnancy Risk Assessment Monitoring System (n = 5548) were analyzed. We calculated adjusted prevalence ratios (aPR) between cannabis use frequency and infant outcomes with Modified Poisson regression.

Approximately 1.7 % and 2.6 % of women reported low and high frequency prenatal cannabis use, respectively. Prevalence of use was higher among women with small-for-gestational age (SGA) (10.2 %) and low birthweight (9.7 %) deliveries, and cigaret counseling and cessation services to help pregnant women avoid tobacco and cannabis use.Lupine (Lupinus sp.) is a valuable source of plant proteins. There is little knowledge on the impact of food processing on composition and sensory properties of lupine products. In this research, we investigated the impact of fermentation with five starters of lactic acid bacteria on the sensory quality and flavor-active compounds in dairy analogues prepared from sweet lupine (Lupinus angustifolius L.). The sensory qualities of unfermented and fermented products were studied with generic descriptive analysis and affective tests. Acids and sugars were analyzed with GC-FID and volatiles with HS-SPME-GC-MS and GC-O. Fermentation increased sourness and 'vinegar' odor and reduced the 'beany' odor and flavor as well as the unpleasantness of flavor. Formation of volatiles during the fermentation was dependent on the starters. However, all fermentations increased the contents of lactic, acetic, and hexanoic acids, while reducing the contents of hexanal, described as 'grassy' in the unfermented lupine sample.