Thygesengram8321

Muscle biochemistry of aquatic birds and mammals varies in accordance with swimming and diving performance, as well as with ontogeny. Similar to other odontocetes, the locomotor muscles (longissimus dorsi) of neonatal melon-headed whales (Peponocephala electra) have low myoglobin content (Mb; 1.06±0.20 g Mb/100 g wet muscle mass; mean ± SE; n=2] and low muscle nonbicarbonate buffering capacity (37.78±3.75 slykes; n=2), representing only 16% of adult Mb (6.64±0.33 g Mb/100 g wet muscle mass; n=5) and 56% of adult muscle nonbicarbonate buffering capacities (66.90±4.80 slykes; n=5). By the juvenile stage, Mb (2.75±0.80⁢ g Mb/100 g wet muscle mass; n=3) is still only 41% of adult levels, but nonbicarbonate buffering capacity (65.61±2.62 slykes; n=3) has matured. Despite the observation that Hawaiian melon-headed whales are not deep divers or long-duration divers, their Mb rivals that found in ziphiids that forage in the bathypelagic zone and monodontids that forage under sea ice. The pelagic lifestyle of melon-headed whales likely requires sustained swimming, such that endurance training could elevate Mb in the locomotor muscle. Indeed, elevated Mb in the locomotor muscles of other pelagic odontocetes has been observed. Unlike deep-diving and Arctic-dwelling odontocetes, melon-headed whales do not achieve mature muscle characteristics before nursing. It is likely that early in life, the hydrodynamic benefits of swimming in echelon position with their mothers minimizes the endurance training of the calves that would otherwise promote rapid elevations in Mb.Oxidative stress is generally understood to be an important mediator of life-history traits, yet the specific relationships between oxidative stress and life-history traits have been difficult to describe because there is often a lack of covariation among biomarkers of oxidative stress. For instance, although oxidative damage to red blood cell (RBC) membranes can lead to pathological conditions (i.e., anemia), in some cases there is not a clear relationship between lipid oxidation and RBC membrane resistance to pro-oxidants. Alternatively, oxidative damage to hemoglobin may be an indirect mechanism contributing to RBC membrane damage. To better understand the mechanisms contributing to oxidative damage and probe new approaches to measuring oxidative stress, we used a series of in vitro and in vivo procedures in zebra finches (Taeniopygia guttata) to explore (1) whether avian RBCs exposed to a pro-oxidant generate fluorescent heme degradation products (HDPs), (2) whether HDPs interact with RBC membranes, and (3) whether HDPs are linked to impaired RBC integrity. selleck We found that finch RBCs exposed in vitro to hydrogen peroxide produced fluorescent HDPs and HDPs associated with RBC membranes. Exposure to hydrogen peroxide also caused a reduction in hemoglobin and an increase in percent methemoglobin (a hemoglobin oxidation product), further indicating hemoglobin degradation. Moreover, HDP fluorescence correlated with impaired membrane integrity and erythrocyte osmotic fragility in vivo. This study suggests that reactive oxygen species may indirectly impair RBC membrane integrity via hemoglobin degradation products that associate with RBC membranes and that HDPs may be an inexpensive and logistically simple tool for measuring oxidative stress.Adaptive plasticity in avian thermal physiology is increasingly apparent, with a well-studied example being metabolic upregulation during cold winters in small birds inhabiting temperate and boreal latitudes. Recent studies have revealed greater variation in the direction and magnitude of seasonal metabolic adjustments among subtropical/tropical birds experiencing milder winters compared with higher-latitude counterparts, suggesting that patterns could vary among years within populations. We quantified seasonal metabolic variation (summer vs. winter) in Kalahari Desert populations of two Afrotropical passerines, the white-browed sparrow-weaver (WBSW; Plocepasser mahali; ∼40 g) and the scaly-feathered weaver (SFW; Sporopipes squamifrons; ∼10 g) over subsequent years (2014-2017). We used flow-through respirometry to measure basal metabolic rate (BMR) and summit metabolism (Msum; maximum cold-induced resting metabolic rate) and quantified seasonal fluctuations in air temperature (Ta) and food abundance (arthropod and grass seed abundance) at the study site. Our data reveal that the direction and magnitude of seasonal metabolic acclimatization vary among years in both species, with the winter BMR of WBSWs ranging from ∼20% lower to 68% higher compared with the summer BMR. In contrast to higher-latitude species, Msum was not related to the cold-limit temperature of birds or to winter minimum Ta at the study site, but interannual variation in BMR and Msum was significantly lower in seasons with lower food abundance in both WBSWs and SFWs. Our data support the idea that patterns of seasonal acclimatization are more variable in birds from lower latitudes and that there is considerable phenotypic flexibility in avian thermal physiology.Objective An impediment to an informed discussion of the impact that campus crime alerts have on college campuses is the dearth of research on the topic. This study explores the composition of campus crime alerts and the ways they convey and (re)produce meanings concerning victimization, perpetration, responsibility, and consent. Methods This study uses an qualitative content analysis of a sample of 3,702 campus crime alerts from 55 universities in the United States. Results Three themes (Central Actor, Overgendering, and Gratuitous Content) emerge. When the victim is portrayed as the alert subject using a passive voice and the crime is framed within a gendered, editorialized narrative, the alert focus shifts to the victim's rather than the perpetrator's identity and behaviors. Conclusions The wording of alerts may compromise the safety of the campus community by reinforcing misguided expectations for behavior and shaping misleading perceptions of risk; considerations for best practices are explored.Background Surveillance of antimicrobial resistance (AMR) requires an international approach with national and local strategies. Our aim was to summarize a retrospective 10-year report of antibiotic resistance of gram-positive and gram-negative bacteria in Mexico. Methods A total of 46 centers from 22 states of Mexico participated. Databases of AMR from January 2009 to December 2018 were included for most species. The 10-year period was divided into five 2-year periods. Results For Staphylococcus aureus, a decrease in resistance in all specimens was observed for erythromycin and oxacillin (p  less then  0.0001 for each). For Enterobacter spp., resistance to meropenem increased for urine specimens (p = 0.0042). For Klebsiella spp., increased drug resistance in specimens collected from blood was observed for trimethoprim/sulfamethoxazole, gentamicin, tobramycin (p  less then  0.0001 for each), meropenem (p = 0.0014), and aztreonam (p = 0.0030). For Acinetobacter baumannii complex, high drug resistance was detected for almost all antibiotics, including carbapenems, except for tobramycin, which showed decreased resistance for urine, respiratory, and blood isolates (p  less then  0.