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The rhizoplane bacterial diversity was reduced on Day 15 while being vastly enhanced on Day 30. Soil variables, including the electric conductivity, redox potential, and soil moisture, along with the soil haloxyfop residue, jointly shape the bacterial community in rhizosphere soil. Electrolytic manganese residue (EMR) contains a large amount of NH4+-N and Mn2+ and can negatively impact the environment. A stabilization treatment of soluble contaminants in the EMR is necessary for its reuse and safe stacking. This study presents experimental results for the stabilization of NH4+-N and Mn2+ in the EMR using phosphogypsum leachate as a low-cost phosphate source and MgO/CaO (PLMC) process. The results demonstrated that the stabilization efficiency of NH4+-N and Mn2+ was 93.65% and 99.99%, respectively, under the following conditions a phosphogypsum leachate dose of 1.5 mL g-1, an added MgO dose of 0.036 g g-1, an added CaO dose of 0.1 g g-1 and a reaction time of 2 h. The stabilization effect of the PLMC process was higher and more cost effective than that of using Na3PO4·12H2O and MgO/CaO. The concentration of NH4+-N and Mn2+ in the leaching liquor decreased to 80 mg L-1 and 0.5 mg L-1, respectively, after the stabilization under the optimum conditions. The stabilization characteristics indicated that NH4+-N was stabilized to form NH4MgPO4·6H2O (struvite) and that Mn2+ was stabilized to form Mn5(PO4)2(OH)4, Mn3(PO4)2·3H2O and Mn(OH)2. PO43--P, F-, and heavy metal ions of the phosphogypsum leachate were removed from the leaching liquor and stabilized in the treated EMR. Mediterranean forests are highly susceptible to wildfires, which can cause several impacts not only within burnt areas but also on downstream aquatic ecosystems. N-Nitroso-N-methylurea in vitro The ashes' washout from burnt areas by surface runoff can be a diffuse source of toxic substances, such as metals, when reaching the nearby aquatic systems, and can be noxious to aquatic organisms. The present work aimed at assessing the ecotoxicological effects of post-fire contamination on two aquatic producers (the microalgae Raphidocelis subcapitata and the macrophyte Lemna minor) through in-situ bioassays, validating the obtained results with the outcomes of laboratory bioassays with surface water collected simultaneously. Four distinct sites were selected in a basin partially burnt (Ceira river basin; Coimbra district, Portugal) for bioassay deployment one site upstream the burnt area in the Ceira river (RUS); three sites located under the influence of the burnt area, one immediately downstream of the burnt area in the Ceira river (RDS) and theexplaining species growth variation; (ii) interaction between metals and/or between metals and other field parameters are likely to modulate the biological responses to the challenges deriving from wildfire runoff. OBJECTIVE To evaluate if a decreasing human chorionic gonadotropin (hCG) between day (D) 1 and D7 is an equal or better predictor of tubal ectopic pregnancy (EP) resolution following methotrexate (MTX) treatment than the current standard of care. STUDY DESIGN This was a retrospective cohort prognostic accuracy study of women with a transvaginal ultrasound (TVS)-confirmed tubal EP (November 2006-December 2015). After single-dose MTX treatment, D4/7 hCG ratios were compared with that of D1/D7 in terms of sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) to predict EP resolution. RESULTS Tubal EP was diagnosed in 301/7350 (4.1%) women who underwent TVS for early pregnancy-related complaints. The patients were managed accordingly expectant, 84/301 (27.9%); MTX, 65/301 (21.6%); surgery, 152/301 (50.5%). A D1/D7 hCG ratio ≤0.85 predicted successful resolution of tubal EPs (P less then 0.001) treated with MTX with sensitivity 0.84 [95% confidence interval (CI), 0.69-0.94]), specificity 0.71 [95%CI, 0.48-0.89], PPV 0.84 [95%CI, 0.69-0.94], NPV 0.84 [95%CI, 0.69-0.94], which is comparable to the prognostic performance of the D4/7 protocol. CONCLUSION In patients with tubal EP carefully selected for and treated with MTX, it may be reasonable to eliminate the D4 hCG in the follow-up algorithm. Biological aerosols play an important role in atmospheric chemistry, clouds, climate, and public health. Here, we studied the morphology and composition of primary biological aerosol particles (PBAPs) collected in the Lesser Khingan Mountain boreal forest of China in summertime using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). C, N, O, P, K, and Si were detected in most of the PBAPs, and P represented a major marker to discriminate the PBAPs. Of all detected particles >100 nm in diameter, 13% by number were identified as PBAPs. We found that one type of PBAPs mostly appeared as similar rod-like shapes with an aspect ratio > 1.5. Size distribution of the rod-like PBAPs displays two typical peaks at 1.4 μm and 3.5 μm, which likely are bacteria and fungal particles. The second most PBAPs were identified as fungal spores with ovoid, sub-globular or elongated shapes with a smooth surface and small protuberances with their dominant size range of 2-5 μm. Moreover, we found some large brochosomal clusters containing hundreds of brochosomes with a size range of 200-700 nm and a shape like a truncated icosahedron. We estimated that mass concentration of PBAPs approximately 1.9 μg m-3 and contributed 47% of the in situ PM2.5-10 mass. The detection frequency and concentration of PBAPs were higher at night than in the daytime, suggesting that the relative humidity dramatically enhanced the PBAPs emissions in the boreal forest. Our study also showed that the fresh PBAPs displayed weak hygroscopicity with a growth factor of ~1.09 at RH = 94%. TEM analysis revealed that about 20% of the rod-like PBAPs were internally mixed with metal, mineral dust, and inorganic salts in the boreal forest air. This work for the first time provides the overview of individual PBAPs from nanoscale to microscale in Chinese boreal forest air. Comprehensive ecological risk assessment is of great significance for the restoration of watershed ecosystem health, and the appropriate and effective assessment method is the premise of ecological risk assessment. In this study, the conceptual model of risk response was developed by identification of ecological risk sources, stressors, endpoints and the corresponding response mechanism as well as the improved TOPSIS model based on Canberra distance and the combinatorial weighting method based on AHP and Critic were combined for the assessment. According to the three aspects of agriculture, industrial and urbanization, the occurrence mechanism of comprehensive ecological risk of rivers in semi-arid areas was analyzed. Furthermore, twenty-four indexes were selected to establish the index system and the Wei River Basin was taken as an example to verify the model. The results of comprehensive ecological risk assessment and stressor analysis showed that the deterioration of water quality (enrichment of heavy metals) and the decrease of benthos integrity were the two main risk factors for the increase of comprehensive ecological risk in Wei River Basin.