Tangeworkman7059

These findings demonstrate that protein-level regulation by the ubiquitin proteasome system is critical for balancing airway cell fates.

Data on the prevalence of clinically significant antigens (Rh, Kell, Kidd, Duffy, MNSs, Lewis, P and Lutheran) among the Indian donor population is sparse.

This prospective study was aimed at determining the prevalence of 21 clinically significant antigens for the first time in the South Indian donor population.

A total of 672 regular O group blood donors were enrolled for Rh (C,c, E, e) and Kell (K) antigens typing. Of these, 188 donors were typed for other clinically significant antigens (k, Kp

, Kp

, Jk

, Jk

, Fy

, Fy

, M, N, S, s, P1, Le

, Le

, Lu

and Lu

).

Antigen frequencies were expressed in percentages. In our study, R1R1 and rr were the most common phenotypes among D+ and D- donors, respectively. Among the Rh antigens, the e antigen was expressed by 97.5% and 100% of D+ and D- donors, respectively. The K antigen was found in only 0.15% of donors. In the Duffy and Kidd blood group system, Fy (a+b+) and Jk (a+b+) were the most frequent phenotypes, respectively. In the MNSs blood group system, M+N+ and S-s+ were the most common phenotypes reported. The Le (a-b+) was found to be the phenotype with the highest prevalence in the Lewis blood group system. The Lu (a-b+) was the only phenotype found in the Lutheran blood group system.

Knowledge regarding the prevalence of antigens in a given population is essential in developing cost-effective in-house panels and a rare donor registry comprising donors typed negative for a high-frequency antigen or a combination of common multiple antigens.

Knowledge regarding the prevalence of antigens in a given population is essential in developing cost-effective in-house panels and a rare donor registry comprising donors typed negative for a high-frequency antigen or a combination of common multiple antigens.Recovered fertilizers (a highly stabilized digestate and ammonium sulphate) obtained from anaerobic digestion of sewage sludge, were used on plot trials with a maize crop, in a comparison with synthetic fertilizers. After three consecutive cropping seasons, the soils fertilized with the recovered fertilizers (RF), compared to those fertilized with synthetic fertilizers (SF), did not show significant differences either in their chemical characteristics or in the accumulation of inorganic and organic pollutants (POPs). The RF ensured an ammonia N availability in the soil equal to that of the soil fertilized with SF, during the whole period of the experiment. Furthermore, no risks of N leaching were detected, and the use of RF did not result in a greater emission of ammonia or greenhouse gases than the use of SF. The agronomic results obtained using RF were equivalent to those obtained with SF (fertilizer use efficiency of 85.3 ± 10 and 93.6 ± 4.4% for RF and SF respectively). The data show that utilising a very stable digestate can be a good strategy to produce a bio-based fertilizer with similar performance to that of a synthetic fertilizer, without environmental risks.Good ecological status is the environmental objective of EU water legislation to be achieved by all European water bodies. However, good ecological status varies depending on national criteria, typology approach, and classification systems used. Since nutrient enrichment is the main cause of river degradation, it is important to establish nutrient criteria that consistently support good ecological status across these influential factors. This study analyzes good ecological status, depending on the typology, classification system and biological element used, and it discusses potential implications of the results for river management. We used a database of 425 sites from northern Spain, corresponding to 11 river types of the Spanish typology derived from physiographic data, or to the four river types resulting from NORTIdiat predictive model, derived from regional diatom reference assemblages. PERMANOVA analysis found significant differences among diatom assemblages across the four river types derived from the NORTIdiat system. Among the classification systems currently in use, or of potential use in the area, the upper P-PO43- threshold, established as the P95 of the class distribution for good ecological status, both NORTIdiat (50.7 μg l-1) and the Multimetric Diatom index (MDIAT; 26.4 μg l-1) were close to proposed thresholds for good status at the EU level. However, this value was much higher for the Specific Polluosensitivity Index (IPS; 118.1 μg l-1). Nutrient thresholds for good status also varied among bioindicators, since the predictive invertebrate-based model NORTI classified 67% of samples with high P-PO43- content in good ecological status, whereas the NORTIdiat classified only 33% of them in good status. Results suggest that current nutrient criteria used to establish good ecological status should be revised, accounting for biological specificity and response of biological elements, to provide a more ecologically coherent approach to preserving or restoring good ecological status.Urban hydrology is characterized by increased runoff and various pollutant sources. We studied the spatio-temporal patterns of stormwater metal (Al, V, Cr, Mn, Fe, Cu, Zn, and Pb) concentrations and loads in five urbanized and one rural catchment in Southern Finland. The two-year continuous monitoring revealed a non-linear seasonal relationship between catchment urban intensity and metal export. For runoff, seasonal variation decreased with increasing imperviousness. The most urbanized catchments experienced greatest temporal variation in metal concentrations the annual Cu and Zn loads in most of the studied urbanized catchments were up to 86 times higher compared to the rural site, whereas Fe loads in the urbanized catchments were only circa 29% of the rural load. Total metal levels were highest in the winter, whereas the winter peak of dissolved metal concentrations was less pronounced. The collection of catchment characteristics explained well the total metal concentrations, whereas for the dissolved concentrations the explanatory power was weaker. Our catchment-scale analysis revealed a mosaic of mainly diffuse pollutant sources and calls for catchment-scale management designs. As urban metal export occurred across seasons, solutions that operate also in cold conditions are needed.Wastewater-based epidemiology (WBE) is utilized globally as a tool for quantifying the amount of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) within communities, yet the efficacy of community-level wastewater monitoring has yet to be directly compared to random Coronavirus Disease of 2019 (COVID-19) clinical testing; the best-supported method of virus surveillance within a single population. This study evaluated the relationship between SARS-CoV-2 RNA in raw wastewater and random COVID-19 clinical testing on a large university campus in the Southwestern United States during the Fall 2020 semester. Daily composites of wastewater (24-hour samples) were collected three times per week at two campus locations from 16 August 2020 to 1 January 2021 (n = 95) and analyzed by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) targeting the SARS-CoV-2 E gene. Campus populations were estimated using campus resident information and anonymized, unique user Wi-Fi connections. Resultant tration.The microbiologically influenced corrosion of 304 stainless steel in the presence of a marine biofilm-forming bacterium Tenacibaculum mesophilum D-6 was systematically investigated by means of electrochemical techniques and surface analyses to reveal the effect of the selective attachment and adsorption of the biofilms on the passivity breakdown of the stainless steel. It was found that the T. mesophilum D-6 was electroactive and could oxidize low valent cations and metal, facilitating the local dissolution of the passive film and the substrate in the film defects, nearly doubling the surface roughness. The biofilms of T. mesophilum D-6 with mucopolysaccharide secreta and chloride ions tended to preferentially adsorb at the defects of the passive film on the steel, yielding non-homogeneous microbial aggregates and local Cl- enrichment there. The adsorption of the bacteria and chloride ions reduced the thickness of passive film by 23.9%, and generate more active sites for pitting corrosion on the passive film and more semiconducting carrier acceptors in the film. The maximum current density of the 304 SS in the presence of T. mesophilum D-6 was over one order of magnitude higher than that in the sterile medium, and the largest pit was deepened 3 times.Systems for regular, preventive maintenance of infrastructure are needed to ensure safe water access globally. Emerging and growing across rural sub-Saharan Africa, professionalized maintenance arrangements feature legal, regulated service providers who maintain infrastructure in exchange for consumer payment through contracts. However, little is understood about the conditions that enable service providers to retain consumer contracts, an important component of their sustainability that indicates consistent demand and payment. This paper uses fuzzy-set Qualitative Comparative Analysis to identify combinations of operational, natural, physical, political, and social conditions enabling high contract retention across 22 implementation cases in Uganda, uncovering 2 pathways to success. In both pathways, consistent expansion by the service provider normalizes concepts such as tariff payment and local government participation increases trust and accountability between the service provider and consumers. The predominant pathway features one additional condition, coordinated sector aid, ensuring consistent implementation and mitigating harmful dependencies. The alternative pathway relies on large user communities and ease of access to those communities to counteract uncoordinated aid. Thus, operational, social, and political conditions may be sufficient to enable high contract retention irrespective of natural and physical conditions. This paper uncovers the combined efforts required of service providers, service authorities, international donors, and local aid actors to ensure the sustainable maintenance of rural water infrastructure for reliable safe water access.Although coastal sediments are major contributors to the production of atmospheric methane, the effects of environmental conditions on methanogenesis and the community of methanogenic archaea are not well understood. Here, we investigated the methanogenesis pathways in nearshore and offshore sediments from the South Yellow Sea (SYS). Moreover, the effects of the supply of methanogenic substrates (H2/CO2, acetate, trimethylamine (TMA), and methanol) and temperature on methanogenesis and the community of methanogenic archaea were further determined. find more Methylotrophic, hydrogenotrophic and acetotrophic methanogenesis were found to be responsible for biogenic methane production in nearshore sediments. In the offshore sediments, methylotrophic methanogenesis was the predominant methanogenic pathway. The changes in methanogenic community structure under different substrate amendments were characterized. Lower diversities were detected in substrate-amended samples with methanogenic activity. Hydrogenotrophic Methanogenium, multitrophic Methanosarcina, methylotrophic Methanococcoide, Methanococcoide or methylotrophic Methanolobus were dominant in H2/CO2-, acetate-, TMA- and methanol-amended sediment slurries, respectively.