Stewartcallesen7846

High phosphorus source concentration inhibited the growth of Mortierella fungi. The biomass (22 g/L) and lipid (14 g/L) yield of Umbelopsis vinacea was the highest among all the tested strains. KEY POINTS • The strain specific P requirements of Mucoromycota depend on the nature of N source. • Yeast extract leads to consistent biomass and lipid yield and fatty acids profiles. • Umbelopsis vinacea showed the highest biomass (22 g/L) and lipid (14 g/L) yield. • High P source amounts inhibit the growth of Mortierella fungi.White spot syndrome virus (WSSV) is one of the most virulent pathogens afflicting shrimp farming. Understanding its influence on shrimp intestinal microbiota is paramount for the advancement of aquaculture, since gut dysbiosis can negatively impact shrimp development, physiology, and immunological response. Thereupon, the data presented herein assesses the influence of WSSV infection and different rearing systems on the intestinal microbiota of Penaeus vannamei. Our study aimed to describe and correlate the composition of shrimp (Penaeus vannamei) gut microbiota, when reared in biofloc and clear seawater, before and (48 h) after WSSV experimental infection. Shrimp were kept in two different systems (biofloc and clear seawater) and experimentally infected with WSSV. Intestine and water samples were characterized by 16S rRNA gene sequencing, before and after viral infection. We observed (i) WSSV induced higher mortality among shrimp reared in biofloc; (ii) WSSV led to a loss of intestinal microbiota heterogeneity, at the genus level, in shrimp kept in clear seawater; (iii) there was a prevalence of Cetobacterium and Bacillus in the intestine of shrimp from both systems; (iv) WSSV did not cause significant changes in intestinal microbiota diversity or richness; (v) regardless of the type of system and time of infection, intestinal microbiota was dissimilar to that of the surrounding water, despite being influenced by the type of system. Therefore, WSSV infection leads to punctual dysbiotic changes in shrimp microbiota, although the virus is sufficiently virulent to cause high mortalities even in well-managed systems, such as a balanced experimental biofloc system. KEY POINTS • WSSV infection leads to a perturbed gut microbiota in shrimp. • WSSV infection greater impacts microbiota of shrimp reared in CSW than those in BFT. • WSSV infection caused higher mortality levels in shrimp reared in BFT than in CSW. • Rearing system influences shrimp gut microbiota composition. Graphical abstract.Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism, adapted to withstand physicochemical stress, and the capacity to thrive in harsh environments. Owing to these characteristics, there is a growing interest in this microbe for industrial use, and the corresponding research has made rapid progress in recent years. Hereby, strong drivers are the exploitation of cheap renewable feedstocks and waste streams to produce value-added chemicals and the steady progress in genetic strain engineering and systems biology understanding of this bacterium. Here, we summarize the recent advances and prospects in genetic engineering, systems and synthetic biology, and applications of P. putida as a cell factory. KEY POINTS • Pseudomonas putida advances to a global industrial cell factory. • Novel tools enable system-wide understanding and streamlined genomic engineering. • Applications of P. putida range from bioeconomy chemicals to biosynthetic drugs.This study investigated the impact of oxygen partial pressure on yeast growth. Saccharomyces cerevisiae cells were exposed to various hyperbaric air conditions from 1 bar to 9 bar absolute pressure (A). Batch cultures were grown under continuous airflow in a 750 mL (500 mL culture) bioreactor and monitored through growth rate and specific yields of ethanol and glycerol. In addition, the concentrations of antioxidant metabolites glutathione (reduced state, GSH and oxidized state, GSSG) and the activity of antioxidative enzymes superoxide dismutases (SOD) and catalases (CAT) were monitored. The results demonstrated that the different oxygen partial pressures significantly impacted the key growth parameters monitored. Compared with atmospheric pressure, under 2 to 5 bar (A), yeast cells showed higher growth rates (μ = 0.32 ± 0.01 h-1) and higher catalase (CAT) concentrations (214 ± 5 mU/g). GSH/GSSG ratio (6.36 ± 0.37) maintained until 6 bar (A) and total SOD (240 ± 5 mU/g) level significantly increased compared with 2 bar (A) until 7 bar (A). Under 6 to 9 bar (A), cell growth was inhibited, and a pressure of 9 bar (A) led to excessive GSSG accumulation (GSH/GSSG = 0.31 ± 0.06). The inhibition of t-SOD (160 ± 3 mU/g) and CAT (62.73 ± 0.2 mU/g) was observed under 9 bar (A). A reference experiment (8 bar (A) N2 + 1 bar (A) air) confirmed that the observed behaviors were entirely due to O2. In addition to their utility in biotechnological process design, these results showed that growth impairment was solely due to oxidative stress induced by excessive oxygen pressure. KEY POINTS • Yeast cells were grown in batch mode under 1 to 9 bar (A) air pressures and up to 5 bar (A) promoted then hindered growth. • The GSH/GSSG ratio was stable up to 5 bar (A) then GSSG accumulated to excess. • Complementary investigations of the activity of SOD and CAT validated growth limitations due to oxidative stress.

The National Comprehensive Cancer Network (NCCN) guidelines recommend examination of a minimum of 12 lymph nodes (LNs) for accurate staging of a single case of colorectal cancer. However, the guidelines do not support the examination of LNs in synchronous colorectal carcinoma (SCC). This study aimed to investigate the association between lymph node yield and the prognosis of SCC patients.

Synchronous colorectal carcinoma patients were selected from the Surveillance, Epidemiology, and End Results (SEER) database over a 10-year interval (2004 to 2013). Systematic dichotomization for optimal cut-off point identification was performed using X-tile. selleck products The baseline for the two LNs groups generated was balanced using the propensity score matching (PSM) method.

A total of 4616 patients met the inclusion criteria. The cut-off number for lymph node retrieved from a single patient was 15 and 12 for the first- and second-time diagnosis of SCC, respectively. Age, T category, N category, tumor grade, tumor site, tumor size, and radiation sequence were not balanced in the two groups.