Stevensonwilladsen4458
Limited information existed on performance tests in Iranian Arab horses.
The objective of this study is to investigate time related changes of cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and oxidative/antioxidant biomarkers in the serum of Arabian horses before and after regular training.
Blood samples were collected from jugular vein of 25 Arabian horses before exercise; 5 h and 18 h after exercise and used to measure the cTnI, ANP, malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), glutathione peroxidase (GPX), and superoxide dismutase (SOD) concentrations. Data analysis was performed using SAS.
Significant time related changes were seen for cTnI, MDA, and GPX concentrations (P<0.05). There were no time significant variations in the concentrations of ANP, FRAP and SOD. The values of cTnI and MDA significantly increased after exercise. The amounts of GPX significantly increased 5 h after exercise and then decreased up to 18 h after exercise.
The results of the present study can be used in future studies in evaluating the health status of Arabian horses. In addition, the present results can be used as primarily described data in the evaluation of Arabian horses.
The results of the present study can be used in future studies in evaluating the health status of Arabian horses. In addition, the present results can be used as primarily described data in the evaluation of Arabian horses.
is widely distributed in tropical and subtropical regions, causing pseudomalaria in pigeons.
The current study aimed to characterize the phylogenetic position of
in pigeons in Sharkia province, Egypt, based on partial sequencing of the
gene as the conserved regions. The "DNA barcode" of the
gene helps in designing primers that can be used to amplify the same gene in the related haemosporidians.
One hundered blood samples were collected from domestic pigeons to identify
by polymerase chain reaction (PCR) and detect its relationship with other related haemosporidians.
Weight losses of 60%, anemia 40%, low growth rates 26.67%, diarrhea 76.67%, dyspnea 66.67%, some neurological symptoms 33.33%, and death 16.67% were observed in the studied birds. Post-mortem examinations showed chocolate-brown appearance of the livers of the birds and congested parenchymatous organs. Microscopical examinations of Giemsa stained blood smears (n=100) revealed a 30% infection rate. The obtained infection percentages were more pronounced in males (35.71%) than females (16.66%) and more in adults (57.14%) than young pigeons (15.38%). The present sequence of
was deposited in GenBank under accession No. MH345964 and shows 100% identity with other related
species in the Sao Paulo Zoo, Brazil (KU131585 and KU131583) and the UK (KX832581 and KX832586).
This study concluded that the accurate diagnosis of
infection in pigeons by specific primers will help with the early treatment of affected cases, especially in the presence of the immature forms, and can thus avoid the noticed clinical signs and the induced pathological lesions mentioned in our study.
This study concluded that the accurate diagnosis of H. coulmbae infection in pigeons by specific primers will help with the early treatment of affected cases, especially in the presence of the immature forms, and can thus avoid the noticed clinical signs and the induced pathological lesions mentioned in our study.
Fowl adenoviruses (FAdVs) are distributed widely throughout the world, and domestic avian species of all ages are susceptible. Fowl aviadenoviruses (FAdVs) can be separated into 5 different species (A-E) with various genotypes and 12 serotypes. Some geno- or serotypes induce hepatitis-hydropericardium syndrome (HPS), inclusion body hepatitis (IBH), and adenoviral gizzard erosion (AGE).
Detect FAdVs serologically and molecularly and sequencing of FAdVs in broiler flocks in Golestan province.
From December 2017 to June 2018 liver tissues and blood samples were collected from 31 broiler flocks suspected of IBH. Polymerase chain reaction (PCR) was applied on liver samples and the positive samples were sequenced and antibody against FAdVs was measured by enzyme-linked immunosorbent assay (ELISA).
Out of 31 flocks, the titers of 29 flocks (93.5%) were high in ELISA test for FAdVs and 22 flocks (70.96%) were positive in PCR test. Sequence analysis indicated the isolates belonged to D and E genotype of adenovirus.
Inclusion body hepatitis caused by FAdVs, are spreading increasingly in broiler flocks of Golestan province and more attention and surveillance programs of breeder and broiler farms are needed to develop preventive measures. Moreover, vaccination of poultry farms in Iran should be considered by more complement studies.
Inclusion body hepatitis caused by FAdVs, are spreading increasingly in broiler flocks of Golestan province and more attention and surveillance programs of breeder and broiler farms are needed to develop preventive measures. Selleck RMC-4550 Moreover, vaccination of poultry farms in Iran should be considered by more complement studies.
infection (salmonellosis) is a zoonotic bacterial disease. Widespread use of antibiotics in livestock and poultry production for different purposes such as treatment and growth promotion has led to the emergence of antibiotic-resistant
, causing treatment of
infections more difficult with each passing year.
To determine the antibiotic resistance prevalence of
serotypes isolated from animals in different provinces of Iran.
To find eligible articles, we searched the international and national electronic databases using appropriate keywords in English and Persian.
After applying predefined criteria, 54 articles reporting antibiotic resistance profiles of
serotypes were included.
isolates were mostly resistant against nalidixic acid (67%), tetracycline (66.9%), and streptomycin (49.6%), followed by trimethoprim/sulfamethoxazole (41.6%) and kanamycin (23.6%). The highest sensitivity was observed against imipenem, meropenem, and cefepime with 1.7%, 1.4%, and 1.9% of all isolates being resistant, respectively.
Results revealed that the prevalence of resistant isolates to nalidixic acid, tetracycline and streptomycin is high and their use must be restricted. In addition, resistance to other antibiotics such as chloramphenicol, ampicillin, cephalothin, cefixime, and enrofloxacin is at an alarming level that calls for attention in the future infection control and antibiotic stewardship programs.
Results revealed that the prevalence of resistant isolates to nalidixic acid, tetracycline and streptomycin is high and their use must be restricted. In addition, resistance to other antibiotics such as chloramphenicol, ampicillin, cephalothin, cefixime, and enrofloxacin is at an alarming level that calls for attention in the future infection control and antibiotic stewardship programs.
Avian pathogenic
(APEC) strains have been associated with various disease conditions in avian species due to virulence attributes associated with the organism.
This study was carried out to determine the
pathogenic characteristics and virulence encoding genes found in
strains associated with colibacillosis in chickens.
Fifty-two stock cultures of
strains isolated from chickens diagnosed of colibacillosis were tested for their ability to produce haemolysis on blood agar and take up Congo red dye. Molecular characterization was carried out by polymerase chain reaction (PCR) amplification of virulence encoding genes associated with APEC.
Eleven (22%) and 41 (71%) were positive for haemolysis on 5% sheep red blood agar and Congo red agar, respectively. Nine virulence-associated genes were detected as follows
(96%),
(52%),
(48%),
(33%),
(21%),
(15%),
(10%),
(2%), and
(2%)
The APEC strains exhibited virulence properties and harbored virulence encoding genes which could be a threat to the poultry population and public health. The putative virulence genes were diverse and different in almost all isolate implying that pathogenesis was multi-factorial and the infection was multi-faceted which could be a source of concern in the detection and control of APEC infections.
The APEC strains exhibited virulence properties and harbored virulence encoding genes which could be a threat to the poultry population and public health. The putative virulence genes were diverse and different in almost all isolate implying that pathogenesis was multi-factorial and the infection was multi-faceted which could be a source of concern in the detection and control of APEC infections.
Canine astrovirus (CAstV) has been considered the primary cause of gastroenteritis in young animals worldwide, while canine vesivirus (CVeV) and canine circovirus (CCiV) are occasionally reported.
This study aimed to investigate the existence and molecular characteristics for these three viruses in Turkey.
Faecal samples from 150 shelter dogs with gastrointestinal problems (127 adults and 23 puppies) were collected and examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis based on the partial sequence of
gene (ORF1b) for CAstV,
gene of CVeV and capsid protein (Cap) and replication associated protein (
) gene of CCiV. Randomly selected positive samples were submitted to sequencing and molecular analyses were conducted based on partial sequences.
It was found that 66% (99/150) of diarrhoeic dogs were positive for CAstV, 3.33% (5/150) for CVeV, and 6% (9/150) for CCiV. Four sub-genotypes for CAstV and two sub-genotypes for CVeV were suggested according to molecular analyses. The phylogenetic relationship of CCiV with other strains obtained from various areas was further demonstrated.
This study emphasizes the importance of emerging viruses for canids, classification of them and their proportional contribution in gastroenteritis cases. We concluded that astrovirus infection must be considered as the major cause of diarrhea in dogs; However, the prevalences of vesivirus and circovirus were relatively low in cases makes them less important in Turkey.
This study emphasizes the importance of emerging viruses for canids, classification of them and their proportional contribution in gastroenteritis cases. We concluded that astrovirus infection must be considered as the major cause of diarrhea in dogs; However, the prevalences of vesivirus and circovirus were relatively low in cases makes them less important in Turkey.The success of conception in buffalo is greatly dependent on precise estrus detection and time of artificial insemination (AI). Various visual, behavioral, biochemical and gyneco-clinical parameters have been tracked closely and a cost-effective combinatorial model has been developed to detect estrus in buffaloes. Pheromones play pivotal roles in reproduction and behavior of mammals. Urine, an easily available biological material which reflects the internal status of an animal, was recruited for profiling the pheromone compounds during the various phases of estrous cycle using gas chromatography-mass spectrometry (GC-MS) analysis. Among the identified compounds, 4-methyl phenol (4-mp, p-Cresol) and 9-octadecenoic acid (Oleic acid) were found to be estrus-specific and would be promising estrus-indicators. Similarly, detection of luteinizing hormone (LH) in urine was also focused to predict the time of ovulation in buffaloes. Partial success has been obtained in the attempt to develop a cost-effective bioassay kit for estrus detection.
