Sinclairworkman0363

This study investigated the effects of Lactobacillus hilgardii (LH), alone or in combination with Lactobacillus plantarum (LP), on the aerobic stability, fermentation quality and dynamics of the bacterial and fungal communities of sugarcane top silage. Results demonstrated that LH and LHLP (LH combined with LP) improved the aerobic stability of sugarcane top silages. As the exposure time increased, the pH values and the contents of lactic acid, acetic acid, as well as propionic acid remained stable in silage treated with LH and LHLP. The abundance of L. hilgardii was enriched and the undesirable microorganisms, such as Acetobacter pasteurianus, Paenibacillus amylolyticus and yeasts like Kazachstania humilis, were suppressed in silages treated with LH and LHLP. In conclusion, LH-treated silage, whether with LP or not, positively impacted the fungal and bacterial microbes. This improved the quality of fermentation, the aerobic stability, and reduced aerobic spoilage in sugarcane top silage.Catalytic co-pyrolysis of water hyacinth and scrap tire experiments were performed to evaluate the feasibility of improving the monocyclic aromatic hydrocarbons production. The production of monocyclic aromatic hydrocarbons increased from 5.31% (sole pyrolysis of water hyacinth) to 13.11% (co-pyrolysis with scrap tire). With use of zeolites, the highest production of monocyclic aromatic hydrocarbons can reach up to 69.18%. Comprehensive comparison on catalytic effects of HZSM-5 and multilamellar MFI nanosheets were provided. With the material to multilamellar MFI nanosheets ratios changes from 21 to 14, the production of monocyclic aromatic hydrocarbons increases significantly from 37.15-69.18%. The average production of monocyclic aromatic hydrocarbons produced by using multilamellar MFI nanosheets were 12.07% higher than that using HZSM-5, indicating the better performance of multilamellar MFI nanosheets in producing monocyclic aromatic hydrocarbons. This work provided a reference for the reuse of water hyacinth and scrap tire over multilamellar MFI nanosheets in energy field.This study focus on the fate of ARGs in the full-scale AD of food wastewater (FWW). Residue was collected from two different full-scale thermophilic AD treating FWW. Ten selected ARGs, including tetracycline resistance genes (tetM, tetX, tetQ, tetH and tetG), sulfonamide resistance genes (sul1 and sul2), quinolone resistance genes (qnrD) and macrolide resistance genes (ermB and ermC), were amplified using quantitative polymerase chain reaction (qPCR). Furthermore, the class 1 integron-integrase gene (intI1) was selected as a representative mobile gene element. Remarkable reduction in the ARGs and intI1 was observed in two-stage (acidogenic-methanogenic) AD, particularly, tetG, tetH, tetM, tetQ, tetX and intI1 not detected. Additionally, significant positive correlation (p less then 0.01) between ARGs and intI1 suggested a strong likelihood of horizontal gene transfer (HGT). Furthermore, stepwise multiple linear regression analysis revealed significant factors related to the fate of individual ARGs and intI1 during AD.In order to investigate the potential effect of sulfur (S) aided composting on NH3 volatilization mitigation, bisphenol A (PBA) and estrogens (estrone, 17β-estradiol, estriol, and 17a-ethinylestradiol) degradation and biosolids stabilization, five treatments of S (i.e., 0, 0.25%, 0.50%, 1.0%, and 2.0%, dry weight basis) were applied to the mixtures of biosolids and wheat straw during the 50 days of composting stabilization process. Results implicated addition of S decreased alkalinity of compost system, mitigated NH3 volatilization, facilitated degradation of bisphenol A and estrogen in biosolids, and improved biosolids stabilization. Compared to control, the S-added treatments reduced nitrogen loss by 29.39%-97.22%, and degraded PBA and estrogens in biosolids by 25.42-72.63% and 21.11-68.14%, respectively, with S additions in range of 0.25-2.0%. In terms of economic efficiency and ecological risk, S addition at ≤0.50% is suggested for composting stabilization of biosolids.Circadian rhythm is a biological cycle that is involved in all processes over 24 h day and night period. Sirtuin 1 (SIRT1) is a 747 amino acid-long class III Nicotinamide adenine dinucleotide (NAD+)-dependent histone that acts as a circadian deacetylase. Here we present a detailed in-silico analysis to address comparative structure-function relationship and interaction pattern of SIRT1-NAD+/Zn+2 and SIRT1NAD+/Zn+2-acetylated histone H4 (H4KAC16) complexes. MD-based ensemble analysis suggested an overall loss of helical content (21.144-17.230%) in H4KAC16-bound SIRT1NAD+/Zn+2 due to conformational readjustments of 32 residues, as compared to SIRT1NAD+/Zn+2. Due to increased flexibility, SIRT1-specific SER275, SER442 and ARG466 residues involved in NAD+ association facilitated in the formation of a transient tunnel (17.77 Å) that was further elongated to 19.25 Å upon SIRT1NAD+/Zn+2 binding to H4KAC16. A close conformation of SIRT1NAD+/Zn+2 was achieved due to binding of H4KAC16 that results in the movement of helical module towards Zn+2 binding module together with Rossmann fold at NAD+ binding region. Furthermore, a 2-fold increase (4.31-8.82 Å) in the measured inter-atomic distance between imidazole nitrogen of conserved HIS363 and NAD+-specific 2'-hydroxyl group of ribose ring was evident in SIRT1NAD+/Zn+2-H4KAC16 complex. At 90 ns time scale, the distance between C6A of adenine ring and C2N of nicotinamide ring was more extended (19.32 Å) in SIRT1NAD+/Zn+2-H4KAC16 as compared to SIRT1NAD+/Zn+2 (11.54 Å). These data suggest that H4KAC16 binding to SIRT1 may coordinate an unusual conformational readjustment of nicotinamide ring at site-b and reposition of HIS363 to facilitate SIRT1-dependent deacetylase activity. Taken together, our findings will help in understanding the precise structural changes occurring in response to SIRT1 deacetylase activity of core histone.In this article, a rapid analytical method for the quantitative determination of the glucose and maltose in the industrial acarbose fermentation was established by the combination application of infrared spectroscopy and chemometrics. The spectra of the 398 acarbose samples were collected by a portable infrared fast analyzer and the concentration of the glucose and maltose in the acarbose fermentation solution were determinate by high performance liquid chromatography (HPLC) as the referent database. Four spectral pretreatment methods, first derivative (FD), second derivative (SD), Savitzky-Golay (SG) convolution smoothing and mean center (MC) were employed to eliminate the optical interference from background and other noise information. The best result was obtained with FD+SG(21, 3)+MC method. The effects of different principal component numbers (PCs) on the parameters were also optimized. Two models of PLS and MLR, were used to predict the concentration of the glucose and maltose. The FD+SG(21, 3)+MC method was chosen as best method, with 12 PCs for glucose and 11 for maltose as optimized parameters. The PLS model was significantly better than the MLR model. Furthermore, both the predicted values and the reference values of glucose and maltose models showed superior linear relationship within the calibration range. The absolute errors of the predicted values and their corresponding reference values of glucose and maltose in the PLS model were within ±0.14 and ±0.35 confidence intervals, respectively. The prediction correct rate was 98.3%, which indicated that the prediction results of model were excellent.This research explores the potential of a portable instrumentation of diffuse reflection infrared Fourier transform (DRIFT) spectroscopy for the in situ characterisation of plastics cultural objects. As sampling has been increasingly questioned in the conservation field, the development of portable devices has been sought. Among them, infrared (IR) spectroscopy in reflection mode has been gaining a powerful position in conservation research. Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) has been widely used for plastics, yet the strong contact required by the technique can make it inappropriate for flexible and/or fragile historic objects. Therefore, in this study, the potential of in situ DRIFT spectroscopy is assessed on both references and historical objects made of the same polymers - polyethylene (PE), polypropylene (PP) and polystyrene (PS). Plastic samples showing different characteristics such as refractive and absorption indexes and topography are also included. These different polymers and surface qualities are discussed as factors influencing the final spectra. In situ DRIFT proved to be very versatile as it could be applied in a variety of plastics and objects' shapes, does not require sampling nor an intimate contact as ATR. Moreover, specific bands and spectral profiles were identified as DRIFT markers of the polymers under study. The acquisition conditions for the in situ analysis were optimized and a pilot spectral database using different IR modes (transmission, ATR and DR) was created. Important information was collected, which allowed the polymer identification of the majority of the historical objects produced between the 1940s and 1980s, from a Portuguese private collection.A study, where uranium was online enriched and laser diode spectrometry was determined, was performed with some bottled mineral water samples. A uranium-imprinted polymer (PMDU) was synthesized and characterized for preparation of a specific adsorbent of UO22+ cations. The homemade system preconcentrated uranium in PMDU resin and determined using an Arsenazo III complex at 650 nm in a combined laser diode spectrometer. All analytical parameters of the system were optimized at 5.5 of the retention pH, 6.0 N HClO4 of eluent concentration, 0.05% of Arsenazo III complex, and a 40 mm coil length. The effect of interferent ions was also investigated and LOD and LOQ values were found to be 0.54 and 1.80 ng mL-1 respectively. Sample throughput was 12 h-1, the preconcentration factor was 50, and RSD% value was 1.1. Certified reference materials of TMDA 52.3 and TMDA 62.2 were quantitatively analyzed and the proposed method was successfully applied to the bottled mineral water samples.Systemic and structural barriers limit dental health for individuals with special healthcare needs (SHCN), who have poorer dental hygiene, higher rates of dental disorders, and less access to oral care. We aimed to understand these barriers directly from the patient and caregiver population as well as review the literature on oral health of individuals with SHCN. We reviewed the literature on individuals and caregivers of those with SHCN to identify barriers to dental healthcare faced by these individuals. We focused on clinical and educational interventions to support clinicians treating this population. For the literature review, PubMed, Google, and Google Scholar were searched. We also relied upon the knowledge gained during the course of routine clinical care and patient advocacy activities. Published manuscripts were searched for the following Medical Subject Heading (MeSH) term "Dental Care for Disabled" and the following subheading pharmacology, adverse effects, ethics, methods, standards, and therapy.