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Moreover, the stressed transgenic lines had significantly lower levels of electrolytic leakage than wild type transgenic lines. These results demonstrate that the CsCYT75B1 gene of sweet orange functions in the metabolism of antioxidant flavonoid and contributes to drought tolerance by elevating ROS scavenging activities.Limited research has been performed to evaluate the effects of high-frequency electrical stunning (ES) methods on the lipid oxidative stability of the meat goose livers. This study was conducted to evaluate the effects of high-frequency-ES current intensities on lipid oxidative stability and antioxidant capacity in the liver of Yangzhou goose (Anser cygnoides domesticus). Forty 92-day-old male Yangzhou geese were randomly divided into five treatments (n = 8). Geese were not stunned (control) or exposed to ES for 10 s with alternating current (AC) at 500 Hz in a water bath. Current intensities were set at 30 V/20 mA (E30V), 60 V/40 mA (E60V), 90 V/70 mA (E90V), or 120 V/100 mA (E120V), respectively. BMS387032 The malondialdehyde level at day 0 was the highest in 120 V (p less then 0.05). Antioxidant enzymes' activity on day 2 was the highest in E60V. The 1, 1-diphenyl-2-picrylhydrazyl free radical (DPPH·) elimination ability was lower in the E120V than that in the E60V at two days and four days postmortem (p less then 0.05). A combination of 60 V/40 mA/ 500 Hz/ 10 s per bird could be applied in the ES of Yangzhou geese to improve the lipid oxidative stability and antioxidant capacity in the livers.The paper presents the concept of mission planning for a short-range tactical class Unmanned Aerial Vehicle (UAV) that recognizes targets using the sensors it has been equipped with. Tasks carried out by such systems are mainly associated with aerial reconnaissance employing Electro Optical (EO)/Near Infra-Red (NIR) heads, Synthetic Aperture Radar (SAR), and Electronic Intelligence (ELINT) systems. UAVs of this class are most often used in NATO armies to support artillery actions, etc. The key task, carried out during their activities, is to plan a reconnaissance mission in which the flight route will be determined that optimally uses the sensors' capabilities. The paper describes the scenario of determining the mission plan and, in particular, the UAV flight routes to which the recognition targets are assigned. The problem was decomposed into several subproblems assigning reconnaissance tasks to UAVs with choosing the reconnaissance sensors and designating an initial UAV flight plan. The last step is planning a detailed flight route taking into account the time constraints imposed on recognition and the characteristics of the reconnaissance sensors. The final step is to generate the real UAV flight trajectory based on its technical parameters. The algorithm for determining exact flight routes for the indicated reconnaissance purposes was also discussed, taking into account the presence of enemy troops and available air corridors. The task scheduling algorithm-Vehicle Route Planning with Time Window (VRPTW)-using time windows is formulated in the form of the Mixed Integer Linear Problem (MILP). The MILP formulation was used to solve the UAV flight route planning task. The algorithm can be used both when planning individual UAV missions and UAV groups cooperating together. The approach presented is a practical way of establishing mission plans implemented in real unmanned systems.3-mercaptopyruvate sulfurtransferase (3-MST) is an enzyme capable of synthesizing hydrogen sulfide (H2S) and polysulfides. In spite of its ubiquitous presence in mammalian cells, very few studies have investigated its contribution to homeostasis and disease development, thus the role of 3-MST remains largely unexplored. Here, we present a clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) induced 3-mst mutant zebrafish line, which will allow the study of 3-MST's role in several biological processes. The 3-mst zebrafish orthologue was identified using a bioinformatic approach and verified by its ability to produce H2S in the presence of 3-mercaptopyruvate (3-MP). Its expression pattern was analyzed during zebrafish early development, indicating predominantly an expression in the heart and central nervous system. As expected, no detectable levels of 3-Mst protein were observed in homozygous mutant larvae. In line with this, H2S levels were reduced in 3-mst-/- zebrafish. Although the mutants showed no obvious morphological deficiencies, they exhibited increased lethality under oxidative stress conditions. The elevated levels of reactive oxygen species, detected following 3-mst deletion, are likely to drive this phenotype. In line with the increased ROS, we observed accelerated fin regenerative capacity in 3-mst deficient zebrafish. Overall, we provide evidence for the expression of 3-mst in zebrafish, confirm its important role in redox homeostasis and indicate the enzyme's possible involvement in the regeneration processes.RNA silencing is a posttranscriptional gene silencing mechanism directed by endogenous small non-coding RNAs called microRNAs (miRNAs). By contrast, the type-I interferon (IFN) response is an innate immune response induced by exogenous RNAs, such as viral RNAs. Endogenous and exogenous RNAs have typical structural features and are recognized accurately by specific RNA-binding proteins in each pathway. In mammalian cells, both RNA silencing and the IFN response are induced by double-stranded RNAs (dsRNAs) in the cytoplasm, but have long been considered two independent pathways. However, recent reports have shed light on crosstalk between the two pathways, which are mutually regulated by protein-protein interactions triggered by viral infection. This review provides brief overviews of RNA silencing and the IFN response and an outline of the molecular mechanism of their crosstalk and its biological implications. Crosstalk between RNA silencing and the IFN response may reveal a novel antiviral defense system that is regulated by miRNAs in mammalian cells.Invasomes are novel vesicular systems that exhibit improved transdermal penetration compared to conventional liposomes. These vesicles contain phospholipids, ethanol, and terpene in their structures; these components confer suitable transdermal penetration properties to the soft vesicles. The main advantages of these nanovesicles lie in their ability to increase the permeability of the drug into the skin and decrease absorption into the systemic circulation, thus, limiting the activity of various drugs within the skin layer. In this paper, several features of invasomes, including their structure, mechanism of penetration, applications, characterization, and potential advantages in dermal drug delivery, are highlighted. Overall, this review suggests that enhanced transdermal penetration of drugs using invasomes provides an appropriate opportunity for the development of lipid vesicular carriers.Defect engineering in photocatalysts recently exhibits promising performances in solar-energy-driven reactions. However, defect engineering techniques developed so far rely on complicated synthesis processes and harsh experimental conditions, which seriously hinder its practical applications. In this work, we demonstrated a facile mass-production approach to synthesize gray titania with engineered surface defects. This technique just requires a simple liquid-plasma treatment under low temperature and atmospheric pressure. The in situ generation of hydrogen atoms caused by liquid plasma is responsible for hydrogenation of TiO2. Electron paramagnetic resonance (EPR) measurements confirm the existence of surface oxygen vacancies and Ti3+ species in gray TiO2-x. Both kinds of defects concentrations are well controllable and increase with the output plasma power. UV-Vis diffused reflectance spectra show that the bandgap of gray TiO2-x is 2.9 eV. link2 Due to its extended visible-light absorption and engineered surface defects, gray TiO2-x exhibits superior visible-light photoactivity. Rhodamine B was used to evaluate the visible-light photodegradation performance, which shows that the removal rate constant of gray TiO2-x reaches 0.126 min-1 and is 6.5 times of P25 TiO2. The surface defects produced by liquid-plasma hydrogenation are proved stable in air and water and could be a candidate hydrogenation strategy for other photocatalysts.Induced pluripotent stem cells (iPSC) have been the focus of several studies due to their wide range of application, including in cellular therapy. The use of iPSC in regenerative medicine is limited by their tumorigenic potential. Extracellular vesicles (EV) derived from stem cells have been shown to support renal recovery after injury. However, no investigation has explored the potential of iPSC-EV in the treatment of kidney diseases. To evaluate this potential, we submitted renal tubule cells to hypoxia-reoxygenation injury, and we analyzed cell death rate and changes in functional mitochondria mass. An in vivo model of ischemia-reperfusion injury was used to evaluate morphological and functional alterations. Gene array profile was applied to investigate the mechanism involved in iPSC-EV effects. link3 In addition, EV derived from adipose mesenchymal cells (ASC-EV) were also used to compare the potential of iPSC-EV in support of tissue recovery. The results showed that iPSC-EV were capable of reducing cell death and inflammatory response with similar efficacy than ASC-EV. Moreover, iPSC-EV protected functional mitochondria and regulated several genes associated with oxidative stress. Taken together, these results show that iPSC can be an alternative source of EV in the treatment of different aspects of kidney disease.Some of the more protective and favorable factors for the development and health in children and teenagers are family and sport, so family involvement in the children's sports activities is vital in their sports process. The purpose of this study was to analyze the verbal behavior (positive, negative, and neutral comments) of family spectators of school-age athletes regarding sociodemographic and sporting variables. The sample consisted of 190 family spectators of 215 male and female (Mage = 11.66; SD = 1.60) football, basketball, and volleyball players. The Parents' Observation Instrument at Sport Events (POISE) was used for the observation and LINCE was used to codify the verbal comments made. After registering 38,829 comments, the results showed statistically significant differences in relation to the comments made and the gender of athletes, geographical area, kind of sport, and the sporting category. The findings highlight that in a competitive environment, the comments made by spectators related to athletes do not seem to be initiators of potentially violent situations but rather are dependent on the atmosphere in question. Further research is required in this area to foster positive conduct relating to grassroots sports.Many chemical reactions of transition metal compounds involve a change in spin state via spin inversion, which is induced by relativistic spin-orbit coupling. In this work, we theoretically study the efficiency of a typical spin-inversion reaction, 3Fe(CO)4 + H2 1FeH2(CO)4. Structural and vibrational information on the spin-inversion point, obtained through the spin-coupled Hamiltonian approach, is used to construct three degree-of-freedom potential energy surfaces and to obtain singlet-triplet spin-orbit couplings. Using the developed spin-diabatic potential energy surfaces in reduced dimensions, we perform quantum nonadiabatic transition state wave packet calculations to obtain the cumulative reaction probability. The calculated cumulative reaction probability is found to be significantly larger than that estimated from the one-dimensional surface-hopping probability. This indicates the importance of both multidimensional and nuclear quantum effects in spin inversion for polyatomic chemical reaction systems.