Searsjosephsen5883

The aim of this study was to investigate the monomer absorption behavior of decellularized dermis and prepare a gradient-type decellularized dermis-polymer complex. Decellularized dermis was prepared using sodium dodecyl sulfate, and its monomer absorption behavior was investigated using three types of hydrophobic monomer with different surface free energies. The results show that monomer absorption depends strongly on the tissue structure, regardless of the surface free energy, and the amount of absorbed monomer can be increased by sonication. Based on these results, we prepared a gradient-type decellularized dermis-poly(methyl methacrylate) complex by controlling the permeation time of the methyl methacrylate monomer and polymerization initiator into the decellularized dermis. The mechanical strength of this complex gradually increased from the dermis side to the polymer side, and combined the physical characteristics of the dermis and the polymer.Equisetum arvense is well known to hold numerous bioactive phytochemicals. In biosynthesis of nanoparticles (NPs), the bioactive compounds existing in natural materials like medicinal fern act as reducing and capping elements and this NPs synthesis process do not comprise of any toxic elements making them advantageous from other NPs synthesis process. After collection, identification and extraction of Equisetum arvense (Ea) aqueous extract, the biosynthesis of AgNPs was achieved followed by its characterization and multi-biopotential activity studies. The UV-visible spectroscopy, confirmed the biosynthesis of Ea-AgNPs. X-ray diffraction configurations (XRD) identified the crystalline nature of the NPs. The Elemental composition of the NPs was elucidated by the energy dispersive X-ray spectroscopy (EDX), and the scanning electron microscopy (SEM) revealed the structure of Ea-AgNPs. Bioactive compounds existing in Ea-extract accounting for Ag + ion reduction, capping and stabilization of NPs was detected by Fourier transform infrared spectroscopy (FTIR). The Dynamic Light Scattering (DLS) and the zeta potential was carried out to know the size and charge of Ea-AgNPs. The Ea-AgNPs exhibited high antidiabetic effect in terms of α-glucosidase inhibition, high cytotoxic effect against HepG2 cell lines along with antibacterial and antioxidant effect. This study reports biosynthesis of Ea-AgNPs using aqueous extract of Ea, its substantial anticancer, antidiabetic, antioxidant and antibacterial effects, which could be advantageous to pharmaceutical industries in the controlling of various diseases including diabetes, cancer, and antibacterial related diseases.The multifunctional nanostructures with superparamagnetic and luminescent properties undergo revolution in the field of bio-nanotechnology. In this article, we reported a facile and efficient one-step modified co-precipitation method to load superparamagnetic Fe3O4 nanoparticle on oxidized nanodiamond (Ox-ND). Subsequently, the as-prepared Ox-ND/Fe3O4 hybrid nanoparticle was surface functionalized with vinyltrimethoxysilane (VTMS) to enhance its compatibility with organic media. The structure, morphology, magnetic, and optical properties of the nanohybrid were systematically investigated. The results confirmed successful loading of crystalline Fe3O4 on the surface of Ox-ND. Ox-ND/Fe3O4 multifunctional hybrid nanoparticle presented strong superparamagnetism (with a saturation magnetization of 67 emu/g at room temperature) and photoluminescence (blue emission) with good chemical reactivity. PrestoBlue assay indicated great biocompatibility of silanized Ox-ND/Fe3O4 in MCF-7 cells even at high concentrations, e.g. 7.2 mg/mL. The hybrid nanoparticle synthesized in this study potentially opens doors for high contrast imaging and targeted delivery applications.TiO2 nanotubes (TNTs) are a promising bone/dental implant surface modification strategy with enhanced bioactivity and local therapeutic functions. However, inconsistencies related to the understanding of the influence of various TNTs characteristics on Ti implant functions, and the multi-factorial inter-dependence of such characteristics, requires an in-depth Quality by Design (QbD) analysis towards optimizing TNTs-modified implants. To this end, an extensive systematic literature search was undertaken to identify the various TNTs characteristics that may influence implant performance. Subsequently, in order to facilitate a QbD analysis, an expert questionnaire survey was carried out to determine the perceived contribution of various TNTs characteristics on an implant's biological, physicochemical, and mechanical performance. To achieve this goal, the Quality Function Deployment method was employed using symmetrical triangular fuzzy numbers to translate qualitative expert opinion into meaningful quantitative information. The results show that pore diameter, inter-nanotube distance and wall thickness are the TNTs characteristics with the most influential effects on the overall implant performance. This pioneering study evaluates perceived importance of various parameters contributing to TNTs functionality, and represents a step forward in the implementation of QbD strategies towards optimizing nano-engineered Ti implants.Ceramic materials such as calcium phosphates (CaPs) with a composition similar to the mineral phase of bones and polymeric polylactic acid (PLA) are potential candidates for the manufacturing of scaffolds to act as bone substitutes and for tissue engineering applications, due to their bioresorbability and biocompatibility. Variables such as porosity, topography, morphology, and mechanical properties play an essential role in the scaffolds response. In this paper, a polymer/ceramic composite filament of 1.7 mm in diameter based on PLA and biphasic calcium phosphates (BCPs) was obtained by hot-melt extrusion in a single screw extruder. LY450139 The particles of BCP were obtained by solution-combustion synthesis, and the PLA used was commercial grade. The BCPs ceramics were characterized by X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopy (TEM), and Brunauer, Emmett, and Teller (BET). It was possible to confirm that the main inorganic phases were hydroxyapatite (HAP) and tricalcium phosphate (TCP) with grain sizes below 100 nm and with high porosity. The Filaments obtained are a bit fragile but were able to be used in fused deposition modelling (FDM) using low-cost commercial printers. The filaments were characterized by SEM and energy dispersive X-ray (EDX). The in-vitro tests of filaments showed deposition of apatite phases on their surface, non-cytotoxic behavior, adequate cell proliferation and cell adhesion.This study shows the application of carbon supported electrodes containing Pt/NiO nanoparticles to catalyze the electrochemical oxidation of glucose in neutral media. link2 In particular, this study describes the effect of the Pt content and type of carbon (carbon black, expanded graphite, or charcoal active) in the reaction layer on this oxidation process in neutral media. Pt/NiO nanoparticles were synthesized by a simple hydrothermal method, and further characterized by scanning electron microscopy (SEM), X-ray diffraction spectroscopy (XRD), and cyclic voltammetry. These nanoparticles were used to modify carbon electrodes. The effectiveness of these electrodes for electrochemical glucose oxidation was evaluated. The results revealed that the catalytic activity of the electrodes depends on the content of Pt/NiO nanoparticles and the type of carbon. The 10% Pt/NiO with 90% loading (use of activated charcoal in the reaction layer) as optimum electrode indicated good stability after 1200 voltammetry cycles. This modified electrode was highly active for glucose oxidation in neutral media, which could be attributed to the presence of Pt/NiO nanoparticles as catalyst and high surface area of activated charcoal on the electrode surface.ZnLi based alloys have been proved as desirable candidates for biodegradable materials accounting for its high mechanical performance and great biocompatibility. However, effects of Li on microstructure and comprehensive properties of Zn alloys are seldom investigated and need to be addressed. Herein, Zn-(0.1-1.4 wt%)Li alloys are fabricated and systematically analyzed. Lath-like Zn precipitates are observed in the primary β-LiZn4 (β) phase of Zn-(0.5-1.4 wt%)Li alloys, leading to the formation of dense β/Zn lamellar structure with an inter-spacing of 0.8 μm. Mechanical tests show that the strengths of the ZnLi alloys have at least tripled due to the formation of dense β/Zn lamellar structure. Early degradation behaviors of the ZnLi alloys in simulated body fluid (SBF) reveal a competitive releasing of Li+ and Zn2+. As the priority of Li+ releasing becomes more obvious with increasing Li content in the alloys, aqueous insoluble Li-rich corrosion products containing LiOH and Li2CO3 form a passivation film on Zn-(0.5-1.4 wt%)Li alloys. Consequently, corrosion rate decreases significantly from 45.76 μm/y of pure Zn to 14.26 μm/y of Zn-1.4Li alloy. Importantly, observations of white light interferometer microscope and transmission electron microscope demonstrate that β phase degrades prior to Zn in the alloys, suggesting that biomedical implants made of ZnLi alloys are likely to degrade completely in human body. Cytotoxicity tests of the alloys exhibit no cytotoxicity in 10% extracts. The most tolerated Zn2+/Li+ concentrations of the alloy extracts to L-929 cells are calculated, which provides guidance for future design of Zn alloys containing Li.As an alternative for polymethyl methacrylate, poly(propylene fumarate) (PPF) has been considered as injectable and biodegradable bone cement; however, its mechanical and biological properties need more attention. Hence, the current study aimed to develop the properties by compositing PPF with magnesium calcium phosphate (MCP) nano-powders. In this regard, the pure PPF was compared with PPF/MCP by evaluating their composition, mechanical properties, hydrophilicity, and biodegradability. Furthermore, their bioactivity in the simulated body fluid (SBF) and, via applying MG-63 cells, their cell interaction, including proliferation, adhesion, differentiation, and mineralization, were assessed. The addition of MCP improved compressive strength and elastic modulus of PPF, e.g., 10 wt% MCP increased them to 32.7 and 403 MPa, respectively. Also, hydrophilicity and biodegradation of PPF were enhanced in the presence of MCP; so that the highest hydrophilicity, 42% higher than PPF, was achieved at the presence of 20 wt% MCP. link3 In this condition, after 21-day immersion in SBF, the surface of the sample was covered with a dense and continuous layer of hydroxyapatite. The composite proliferation, adhesion, differentiation, and mineralization of MG-63 cells improved in comparison to the pure PPF. Hence, controllable strength and biodegradation of the composite, along with its proved bioactivity and osteoconductivity, make PPF/MCP as a candidate for bone therapeutic application.In this work, titania nanotube arrays (TiO2-NTs) were prepared by anodization, and the Eu(III) complexes (Eu (TTA)3 phen with 2-thenoyltrifluoroacetone (TTA) and 1, 10-phenanthroline (phen)) were successfully coated onto the walls of the nanotubes. When a solution of glucose, cholesterol or triglycerides was dropped onto Eu(III) complex-modified TiO2-NTs, the fluorescence intensity of this material changes (glucose enhances fluorescence, cholesterol and triglycerides quench fluorescence). These phenomena are explained via an energy transfer process. The sensitivity of the fluorescence intensity to glucose, cholesterol or triglycerides concentration enables design of a multifunctional solid sheet-like detector. Under optimized experimental conditions, the change in fluorescence intensity ratio (ΔF/F0) is linear with the concentration of glucose, cholesterol or triglycerides. To test the utility of the detector, glucose in orange juice, cholesterol in milk powder, and triglycerides in coconut oil were measured using this method and the results were in good agreement analytical data provided by a food testing company.