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Heat mediated suppression of neural activity was optically validated in single cell level. The present study will be helpful to understand the emerging photothermal neuromodulation technology.

Heat mediated suppression of neural activity was optically validated in single cell level. The present study will be helpful to understand the emerging photothermal neuromodulation technology.

The rodent vibrissal (whisker) systcnsorimotor integration and active tactile sensing. Experiments on the vibrissal system often require highly repeatable stimulation of multiple whiskers and the ability to vary stimulation parameters across a wide range. The stimulator must also be easy to position and adjust. Developing a multi-whisker stimulation system that meets these criteria remains challenging.

We describe a novel multi-whisker stimulator to assess neural selectivity for the direction of global motion. The device can generate repeatable, linear sweeps of tactile stimulation across the whisker array in any direction and with a range of speeds. A fiber optic beam break detects the interval of whisker contact as the stimulator passes through the array.

We demonstrate the device's function and utility by recording from a small number of multi-whisker-responsive neurons in the trigeminal brainstem. Neurons had higher firing rates in response to faster stimulation speeds; some also exhibited strong direction-of-motion tuning.

The stimulator complements more standard piezo-electric stimulators, which offer precise control but typically stimulate only single whiskers, require whisker trimming, and travel through small angles. It also complements non-contact methods of stimulation such as air-puffs and electromagnetic-induced stimulation. Tradeoffs include stimulation speed and frequency, and the inability to stimulate whiskers individually.

The stimulator could be used - in either anesthetized or awake, head-fixed preparations - as an approach to studying global motion selectivity of multi-whisker sensitive neurons at multiple levels of the vibrissal-trigeminal system.

The stimulator could be used - in either anesthetized or awake, head-fixed preparations - as an approach to studying global motion selectivity of multi-whisker sensitive neurons at multiple levels of the vibrissal-trigeminal system.

Transcription factor-based forward programming enables the efficient generation of forebrain excitatory and inhibitory neurons from human pluripotent stem cells (hPSCs). This provides an opportunity to study stimulation-response patterns in highly defined neuronal networks in a controlled and customizable in vitro environment.

Cell populations composed of defined ratios of excitatory and inhibitory neurons were generated by forward programming genome-edited human hPSCs carrying the inducible transcription factors NGN2 and ASCL1/DLX2, respectively. These populations were cultured on multi-electrode arrays (MEAs), and population responses elicited by distinct spatial and temporal stimulation patterns were analyzed. In parallel, in silico network models fed with neuronal parameters obtained from the in vitro cultures were developed to explore potential mechanisms underlying experimental observations.

Neuronal cultures developed network-level electrophysiological activities with pronounced synchronized netwonses to electric stimuli. It also illustrates how the combined use of biological and in silico neuronal networks can support the development of mechanistic hypotheses underlying network responses to specific stimuli.

The present study demonstrates the prospect of the iPSC technology for conducting personalized in vitro studies of human neuronal networks and their responses to electric stimuli. It also illustrates how the combined use of biological and in silico neuronal networks can support the development of mechanistic hypotheses underlying network responses to specific stimuli.Marketing pressure on teenagers when it comes to promoting unhealthy foods and food brands is a significant public health concern. Teenagers are aggressively targeted by food marketing messages, yet a research gap exists when it comes to the engagement by teens with this marketing in real world settings, and specific techniques (or power) used to capture their attention. This exploratory study engages in participatory research to explore the persuasive power and platforms of exposure of teen-targeted food marketing. Using an innovative smartphone app called "GrabFM!" ("Grab Food Marketing!"), teens ages 13-17 (n = 62) identified and tagged examples (n = 339) of targeted food marketing (from mainstream and digital media, and the built environment) over a 7-day period, providing information on the food brand, product, platform, and indicators (i.e., persuasive techniques). Results revealed the top brand (FritoLay, 8.3%), food product category (candy/chocolate, 23.3%), platform of exposure (Instagram, 76.4%), and indicator (visual style, 52.5%) identified by teens. Insights were also gained into the intersection of gender and platform, gender, age and indicators (older teens 15+ more likely to report multiple indicators per ad), and co-occurrence of indicators (majority of ads tagged with one indicator only). The results of this study provide guidance on the power, platforms and brands that teens felt uniquely spoke to them. When it comes to monitoring efforts, it is useful to know that Instagram commands teenagers' attention and that marketing power resides in particular indicators (visual style, special offer, theme), which teenagers appear to readily and consistently identify.The P3 component (P300, P3b) is considered to be an effective index of attention and categorization processes when elicited in a visual oddball task, specifically reflecting the selection of a rare target item among frequent non-targets. Researchers have proposed that target categorization is guided by representations of target features held in working memory (WM), thus guiding attention and categorization processes to distinguish targets from non-targets. Although WM is theorized to have visuospatial, verbal and executive function components, most studies do not investigate how these WM components contribute to the P3. This study uses an individual differences approach to determine whether correlations between WM capabilities and P3 amplitudes indicate a common underlying cognitive construct. Participants (n = 140) completed an 80/20 visual oddball task to elicit the P3 as well as independent visual working memory (VWM), spatial working memory (SPWM), and executive function (task switching (TS) and digit symbol substitution (DSS)) tests. Results indicated that measures of executive function, DSS and TS, but not VWM or SPWM ability, correlated with and predicted faster task response times and greater P3 amplitudes. RT and WM measures were not correlated with P3 fractional area latencies. These results support context updating theory. Executive function WM availability, whether as a property of the participant's processing system or based on task demands, plays a functional role in the P3 and an important role in efficient visual categorization and goal-directed learning.

To describe characteristics, hospitalization, and death for reported cases of SARS-CoV-2 infection in the Oklahoma City tri-county area.

We extracted notified cases of SARS-CoV-2 infection for our study area and used descriptive statistics and modeling to examine case characteristics and calculate the odds of hospitalization and death in relation to a range of explanatory variables.

Between March 12th, 2020 and February 28th, 2021, 124,925 cases of SARS-CoV-2 infection were reported from the study region. Being male, White or Black/African American, aged 50 years or older, presenting with apnea, cough, and shortness of breath, and having diabetes was associated with increased odds of hospitalization. The odds of dying were significantly associated with being Black/African American, presenting with cough and fever, having kidney disease and diabetes and being aged 70 years or older.

The first cohort analysis of SARS-CoV-2 positive individuals in the Oklahoma City tri-county area confirms comorbidities and age as important predictors of COVID-19 hospitalization or death. As a novel aspect, we show that early symptoms of breathing difficulties in particular are associated with hospitalization and death. Initial case assessment and SARS-CoV-2 guidelines should continue to focus on age, comorbidities, and early symptoms.

The first cohort analysis of SARS-CoV-2 positive individuals in the Oklahoma City tri-county area confirms comorbidities and age as important predictors of COVID-19 hospitalization or death. As a novel aspect, we show that early symptoms of breathing difficulties in particular are associated with hospitalization and death. Initial case assessment and SARS-CoV-2 guidelines should continue to focus on age, comorbidities, and early symptoms.

Medical students' white coats were found to harbor harmful organisms. This could be due to non-compliance to white coat hygiene measures. Therefore, we aim to develop and validate a questionnaire to assess the of knowledge, attitude, and practice (LAUNDERKAP) of white coat use among medical students in Malaysia.

This study was conducted in 4 local medical schools. https://www.selleckchem.com/products/CAL-101.html LAUNDERKAP was developed via literature review and had 3 domains attitude, knowledge, practice. An expert panel assessed the content validity and clarity of wording. LAUNDERKAP was then piloted among 32 medical students. To test construct validity and internal consistency, 362 medical students were approached. Construct validity was assessed using exploratory factor analysis. Internal consistency was evaluated using Cronbach alpha for attitude and practice, while Kuder-Richardson 20 (KR-20) was used for knowledge.

A total of 319 of 362 students responded. Exploratory factor analysis extracted 1 factor each for attitude and knowledge respectively, and 3 factors for practice. Cronbach alpha for attitude was 0.843 while KR-20 for knowledge was 0.457. Cronbach alpha for practice ranged from 0.375 to 0.689. The final LAUNDERKAP contained 32-items (13 attitude, 9 knowledge, 10 practice).

LAUNDERKAP had adequate psychometric properties and can be used to assess KAP of medical students towards white coat use.

LAUNDERKAP had adequate psychometric properties and can be used to assess KAP of medical students towards white coat use.

To identify key socio-demographic, lifestyle, and health predictors of self-reported coronavirus disease 2019 (Covid-19) history, examine cardiometabolic health characteristics as predictors of self-reported Covid-19 history and compare groups with and without a history of Covid-19 on trajectories in cardiometabolic health and blood pressure measurements over time, among United States (U.S.) older adults.

Nationally representative longitudinal data on U.S. older adults from the 2006-2020 Health and Retirement Study were analyzed using logistic and mixed-effects logistic regression models.

Based on logistic regression, number of household members (OR=1.26, 95% CI 1.05, 1.52), depressive symptoms score (OR = 1.21, 95% CI 1.04, 1.42) and number of cardiometabolic risk factors or chronic conditions ("1-2" vs "0") (OR = 0.27, 95% CI 0.11, 0.67) were significant predictors of self-reported Covid-19 history. Based on mixed-effects logistic regression, several statistically significant predictors of Covid-19 history were identified, including female sex (OR = 3.