Ryttermcfadden6743

PA and 2-EH were further utilized through the protocatechuic acid metabolic pathway and β-oxidation via protocatechuic acid and 2-ethylhexanoic acid, respectively. The application potential of strain RL-JC02 was confirmed through the bioremediation of artificial DEHP-contaminated red soil showing 91.8% DEHP degradation by strain RL-JC02 within 30 d. The kinetics analysis of DEHP degradation by strain RL-JC02 in soil demonstrated that the process followed the modified Gompertz model. Meanwhile, the cell concentration monitoring of strain RL-JC02 in soil with absolute quantification polymerase chain reaction (qPCR) suggested that strain RL-JC02 survived well during bioremediation. This study provides sufficient evidence of a robust degrader for the bioremediation of PAE-contaminated red soil.Microplastics (MPs) lipophilic nature and widespread distribution raises concerns due to their increasing presence in the marine environment and their ability to adsorb organic contaminants, as being potential vehicles for transport and potential source of accumulation of organic contaminants by marine organisms. The organic UV-filter, oxybenzone (BP-3) is a constituent of sunscreens and personal care products, entering the marine environment either by direct contact with swimmers or by wastewater effluents. In this study the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics with and without adsorbed BP-3 were investigated in the peppery furrow shell clam, Scrobicularia plana. LDPE microplastics with a size range of 11-13 μm were previously contaminated with an environmentally relevant concentration of BP-3 (82 ng g-1). S. plana individuals were exposed to a concentration of 1 mg L-1 of microplastics with and without BP-3 adsorbed in a water-sediment exposure system for 14 gestive gland and that biomarkers alterations are apparently more related to the toxicity of BP-3 adsorbed than virgin MPs alone.Zoxamide is a recently discovered chiral fungicide that applied to agricultural production, but the potential environmental risk may be underestimated because the risk posed by either enantiomer has not been adequately assessed. Therefore, systemic evaluation of zoxamide was first carried out at the enantiomeric level. Enantioselective bioactivity against target pathogens (Phytophthora capsici Leonian, Alternaria solani, Botryis cinerea, Colletotrichum gloeosprioides Penz, Phytophthora sojae Kaufmann & Gerdemann) was explored, and the order of the bioactivity was R-zoxamide >Rac-zoxamide >S-zoxamide, with a 9.9- to 140.0-times difference between two enantiomers. Molecular docking simulation was utilized to clarify the mechanism underlying the observed differences in enantioselective bioactivity, and the result indicated that a difference of Van der waals force between R/S-zoxamide and the specific receptor gave rise to the different antifungal activity. The enantioselective toxicity result demonstrated that R-zoxamide had 4.9- to 10.8- times greater acute toxicity to Selenastrum capricornutum and Daphnia magna than S-zoxamide. S-zoxamide degraded faster under aerobic condition in all three types of soils, giving rise to an enrichment of high-risk R-enantiomer. Under anaerobic condition, however, no significant difference in dissipation rate was observed between two enantiomers. R-zoxamide was 1.5- to 3.5-times more bioactive and 1.1- to 1.5-times more toxic than Rac-zoxamide, which means developing R-zoxamide instead of racemate is a potential way to reduce pesticide dosage without loss of efficacy against target organisms and that an inactive isomer would no more be released to the environment. This study may have implications for better practical application and environmental risk assessment of zoxamide enantiomers.Quantitative information relating sewerage infrastructure schemes to microbial water quality improvements in recreational and shellfish harvesting areas is lacking. In this study, we assessed the effect of two sewerage schemes on concentrations of faecal indicator organisms (FIO) in Chichester Harbour, an important oyster fishery and water recreation area in the UK. The sewerage schemes comprised the installation of activated sludge and UV disinfection plants and increase in the storage capacity of storm tanks at sewage treatment works that discharge to tidal waters. Analysis of FIO data covering the period 2007-2018 indicated log-order reductions in FIO concentrations in the harbour after the sewerage schemes, which was reflected by better compliance with the E. coli and enterococci limits for "excellent" of the Bathing Waters Directive. Mean concentrations of E. coli in shellfish reduced ≤0.5log10 and compliance of commercial shellfish beds with the limits of Regulation (EC) No 854/2004 either maintained or upgraded to class B status during the 11-year period. However, compliance with the guideline E. coli standard of the Shellfish Water Protected Areas Directions was not consistently achieved. We suggest that better harmonisation of monitoring practices used in the various statutory programmes would help in understanding if the observed discrepancy in FIO compliance between waters and shellfish is due to actual pollution levels at compliance sites or other factors. Nevertheless, this study demonstrates that fortnightly sampling can provide data to evidence long-term water quality improvements following sewerage schemes.The interaction between soil properties and plant community determines the efficacy to restore the degraded grassland via improving soil properties. We conducted a field survey to investigate the relationship between plant community composition and soil physical and chemical properties alongside different degradation stages of alpine grassland. Results showed that with the increasing severity of degradation, the dominant plants shifted from sedge and graminoid species, respectively, to forbs species in alpine meadow (AM) and alpine steppe (AS). selleck Species richness and diversity indices were unchanged until the severely degraded stage in both AM and AS. Forward selection showed bulk density (BD) and ammonia nitrogen (NH4+) at 10-20 cm, pH and the ratio of soil organic carbon to total nitrogen (C/N) at 0-10 cm were the four important variables being responsible for the species community variation alongside degradation of AS, which explained 18.7% of the total variance. Soil nitrate nitrogen (NO3-) and C/N at 20-30 cm, NH4+ at 10-20, and BD at 0-10 cm were the important variables in driving the community variance alongside degradation stages, which only explained 9.