Rosenkildehvidberg4201

The spectral window observed ranged from 300 to 3800 cm-1. Several characteristic Raman bands were found, namely, at 710 cm-1 (NO3-) and 1040 cm-1 (NO3-) for AN; 1440-1470 cm-1 (CH) and 2800-3000 cm-1 (CH) for FO; 3000-3500 cm-1 (OH) for water; and 615 cm-1 (CCl), 1254 cm-1 (CH), 1400 cm-1 (CH2) and 1600 cm-1 (aromatic ring) for polyvinyl chloride (PVC, electrical tape). The effect of the AN concentration and integration time on the total and net Raman intensities, relative standard deviation, signal-to-noise ratio and relative limit of detection was evaluated. The relative limit of detection of AN in water was 0.1% (1 mg/g), and absolute limit of detection was 1.0 μg. The optimum integration time (≈10 s) for the Raman sensor to capture the analyte signals was estimated based on the Raman figures of merit as a function of the integration time. Simultaneous detection of biomarkers and biomolecules with great analytical performance still is challenging. A simple fluorometric dual-functional aptasensor was designed to detect Lysozyme (LYS) and adenosine triphosphate (ATP) as models of a protein and a small molecule simultaneously. The sensing principle of the aptasensor is based on the interactions between cobalt oxyhydroxide CoOOH nanosheets as fluorescence quencher and carbon dots (CDs) as fluorophores. Daurisoline The aptamer labeled with CDs was able to assemble on CoOOH nanosheets and consequently, the fluorescence signal was quenched. With addition target analytes to the system, the aptamers folded around of targets with a strong and specific affinity. Therefore, the labeled aptamer with CDs was detached from CoOOH nanosheets and the fluorescence signal was restored. The fluorescence spectral overlap of these two CDs is the main limitation for the simultaneous analysis. The least squared support vector machine (LS-SVM) was applied to resolve this problem. Under optimal conditions, when LS-SVR was used, detection limits were found 4.0 and 1.8 nmol L-1 for ATP and LYS. The parallel biosensor is capable of monitoring ATP and LYS levels in the biological samples with satisfactory results. Using Hg2+-promoted deprotection reaction, we have developed a new fluorescent turn-on sensor 2TS based on bithiophene fluorophore for Hg2+ detection. The sensing mechanism of 2TS towards Hg2+ was strongly proved by 1H NMR, FTIR, HRMS, UV-vis and fluorescence spectra. Remarkly, 2TS towards Hg2+ in 100% aqueous solution shows high sensitivity with a low detection limit of 19 nM, superior selectivity and ultra-rapid response of 20 s during a wide sensing pH range from 4 to 10. Taking advantage of the excellent properties, the low-cost sensor 2TS-based filter paper/TLC test strips were fabricated for visual, immediate and quantitative detection of Hg2+ in water, proving its applicability towards sensitive in-situ and on-site detection. Meanwhile, 2TS showed high analytical performance for Hg2+ detection in water, seafood as well as human urine samples. Moreover, thanks to the good water solubility, negligible cytotoxicity, good biocompatibility and cell-membrane permeability, 2TS was further applied to effectively image Hg2+ in live cells. Furthermore, the developed sensor 2TS acted as good fluorescent display material for Hg2+ with obvious color change. The surface-enhanced Raman spectroscopy (SERS) is a method known for its effectiveness in detecting and identifying microorganisms, that was employed to differentiate various bacterial strains both at genus and species level. In this work, we have examined five species belonging to Streptococcus genus, namely S. pneumoniae, S. suis, S. pseudopneumoniae, S. oralis, and S. mitis. Additionally, we conducted SERS experiments on ten S. pneumoniae strains, representing different capsular types. In all of cases we obtained unique SERS signals being spectroscopic fingerprints of bacterial strains tested. Moreover, the principal component analysis (PCA) was performed in order to prove that the spectra of all studied strains can be well separated into five (in case of streptococcal strains) or ten (in case of pneumococcal serotypes) groups. In both investigated situations, the separation at the level of 95% was achieved, proving that SERS-PCA-based method can be used for reliable and fast identification of different strains belonging to the Streptococcus genus, including encapsulated pneumococcal isolates. V.Chemoresistance including intrinsic and acquired anticancer drug resistance continues to be a primary hindrance towards curative cancer treatment. Therefore, deciphering the underlying molecular mechanisms is of paramount importance required towards the overcoming of chemoresistance. Cumulative evidence revealed that long non-coding RNAs (lncRNAs) play a pivotal role in conferring anticancer drug resistance upon a broad spectrum of cancers. Hence, numerous lncRNAs are recognized as novel biomarkers and therapeutic targets in the diagnosis and treatment of malignancies, which urges us to comprehensively delineate the critical functions of lncRNAs in chemoresistance. In this respect, we herein succinctly elucidate the molecular mechanisms by which lncRNAs modulate their downstream targets to mediate cancer chemoresistance. Therefore, the current review may provide a significant basis for the future conquering of chemoresistance via targeting lncRNAs in cancer therapeutics. Enterotoxigenic Escherichia coli (ETEC) F4 causes diarrhea in infants and weaned piglets. The technique of isobaric tags for relative and absolute quantitation (iTRAQ) was used in this study to determine the differentially expressed proteins in porcine intestinal epithelial cells (IPEC-J2) after pretreatment with Lactobacillus plantarum (LP) followed by challenge with ETEC F4. A total of 4771 proteins were identified in IPEC-J2 cells, with 90, 105, and 134 differentially expressed proteins in cells exposed to ETEC, LP, and LP + ETEC, respectively. The COG analysis divided the identified proteins into 20 categories. The GO and KEGG annotation indicated that most of the differentially expressed proteins were enriched in various biological metabolism including cell cycle control, cell division and differentiation. Additionally, western blotting analyses confirmed the reduced abundance of selected proteins of the mTOR and MAPK signal pathways affected by ETEC F4. Moreover, LP pretreatment increased JNK activation in IPEC-J2 cells infected with ETEC F4.