Robersondehn2258
Moreover, there was a decrease in Ki-67 expression and MVD, 21 days after treatment with apatinib. The results also showed that apatinib had no effect on the levels of the VEGFR-2, ERK1/2 and AKT proteins although there was a significant decrease in the expression of phosphate VEGFR2 (p-VEGFR2), phosphate AKT (p-AKT) and phosphate ERK1/2 (p-ERK1/2).
Apatinib inhibits the proliferation and migration of pancreatic cancer cells, blocking growth and angiogenesis in transplanted tumors. In addition, the underlying mechanism may involve phosphorylation of the PI3K/AKT and ERK1/2/MAPKs signaling pathways.
Apatinib inhibits the proliferation and migration of pancreatic cancer cells, blocking growth and angiogenesis in transplanted tumors. In addition, the underlying mechanism may involve phosphorylation of the PI3K/AKT and ERK1/2/MAPKs signaling pathways.
An early indicator for monitoring the effect of adjuvant treatment after lung cancer surgery is urgently needed. The study was to explore the effects of epithelial cell adhesion molecule (EpCAM) of circulating tumor cells (CTCs) in NSCLC patients with postoperative adjuvant chemotherapy.
Two drugs (platinum-containing chemotherapeutics + platinum-free chemotherapeutics) first-line chemotherapy regimen were given after surgery. MRNA of EpCAM was detected. Chest computed tomography, head computed tomography and abdominal B-ultrasound were reviewed before the first and third chemotherapy.
EpCAM in CTCs from peripheral blood between the recurrent group and the non-recurrent group at 1 day before surgery, first, second and third adjuvant chemotherapy were no significant differences (P>0.05). Only one day before the fourth adjuvant chemotherapy treatment, it showed significant difference between the recurrent group and the non-recurrent group (P=0.008). There was a significant difference between the time of imaging diagnosis of recurrence or metastasis and the time of monitoring the expression level of EpCAM in CTCs from peripheral blood (P<0.0001).
EpCAM in CTCs from peripheral blood during postoperative adjuvant chemotherapy was related to recurrence or metastasis of NSCLC patients.
EpCAM in CTCs from peripheral blood during postoperative adjuvant chemotherapy was related to recurrence or metastasis of NSCLC patients.
The therapeutic strategies and prognosis of local advanced and metastatic lung cancer have been extensively investigated. However, the prognosis of early-stage lung cancer patients undergoing radical surgery has not been fully studied due to the difficulties in follow-up and assessment.
We recruited 447 stage I-III lung adenocarcinoma (LUAD) patients who underwent radical surgery and investigated the influence of main driver gene mutations and clinicopathological factors on patient overall survival (OS). Cancer tissue samples were collected retrospectively and mutational status and tumor mutational burden (TMB) were determined by whole-exome sequencing (WES).
Distinct stage-dependent mutational frequency was revealed in main driver genes including
,
,
,
,
and
. Patients with TP53 mutations exhibited a trend of better survival than those with wild type
(P=0.066), and
mutations exhibited worse survival in stage III patients (P=0.031).
mutations eliminated the across-stage difference in survival, which was still present in other wild type and mutant driver genes. Furthermore, patients with wild type TP53 appeared to have significantly worse survival than patients with other wild type driver genes in stage I (P<0.001). TMB cannot stratify the survival of LUAD patients in stage I-III. Age, gender, smoking status, smoking years, prior cancer history and cancer location had no stratification effect on patient survival, while T grading (P<0.001) and N grading (P<0.001) had significant stratification on survival.
,
and
mutational status influenced the prognosis of stage I-III LUAD. T and N grading also stratified the patient survival. T grading was an independent risk factor.
TP53, EGFR and STK11 mutational status influenced the prognosis of stage I-III LUAD. T and N grading also stratified the patient survival. T grading was an independent risk factor.
In our previous study, missense mutations in the Notch1 gene were found in chemotherapy-resistant esophageal squamous cell cancer (ESCC) patients. In this study, we explored changes in the interaction between Notch1 and DLL4 resulting from missense mutations.
Bioinformatics analysis was performed to assess and compare the different biological structures and functions of wild type (WT) and mutation type (MT) sequences of Notch1. A genetic information search was performed, and the results were analyzed using in silico modeling. Homology modeling of the Notch1 protein was carried out using Swiss-Model software, and modeling of site-directed mutations was carried out using PyMOL software to observe the protein structure. The Notch1-DLL4 ligand-receptor complex protein model was constructed, Wincoot software was used to determine site-directed mutations, and a protein-ligand interaction profiler (PLIP) was used to calculate the noncovalent interactions in the complex.
The mutation site was located in the region where Notch1 binds to DLL4. A careful examination of the in silico structural model revealed that the mutation caused an alteration in the surface charge, and the water-bridge bonds of the interaction between Notch1-DLL4 increased in number from 5 to 7.
Notch1 gene missense mutation leads to an increase in the number of water-bridge bonds, thus enhancing the Notch1-DLL4 interaction, which may lead to tighter Notch1-DLL4 binding, either making the pathway easier to activate or increasing the length of time it is active.
Notch1 gene missense mutation leads to an increase in the number of water-bridge bonds, thus enhancing the Notch1-DLL4 interaction, which may lead to tighter Notch1-DLL4 binding, either making the pathway easier to activate or increasing the length of time it is active.
Understanding angiogenesis in prostate cancer is essential. LNCaP prostate xenograft tumors are androgen responsive and closely mimic clinical disease. Orthotopic animal models replicate aspects of the cancer microenvironment and are more clinically relevant than subcutaneous models. Comparative studies investigating angiogenesis using contrast-enhanced ultrasound (CEUS) imaging in subcutaneous and orthotopic mouse models of prostate cancer have not been performed.
Tumor microcirculation and perfusion in subcutaneous and orthotopic LNCaP xenograft Balb/c athymic nude mice models were compared by investigating microbubble wash-in with CEUS.
The take rate of subcutaneous and orthotopic tumors were 58.3% and 68.2%, respectively. On CEUS, orthotopic prostate tumors enhanced more rapidly than subcutaneous tumors. check details Mean arrival-time (Atm) for subcutaneous tumors, orthotopic prostate tumors, and kidney were 4.21±1.86, 1.72±0.79, and 0.73±0.12 s, respectively. Mean Atm was significantly longer for subcutaneous tNCaP xenografts.
These findings demonstrate that orthotopic LNCaP xenografts better recreate a pro-angiogenic microenvironment than subcutaneous LNCaP xenografts.
In our clinical study, 11% of the patients with early-stage cervical cancers had different degrees of vaginal bleeding, which required a preoperative intervention. We set to assess the efficacy and safety of preoperative high-dose rate vaginal ovoid brachytherapy (HDR-VOBT) for the treatment of vaginal bleeding in women with early cervical cancer.
We retrospectively identified and reviewed patients with vaginal bleeding and early-stage cervical carcinoma, treated between January 2011 and December 2014 (median follow-up of 69 months). Of the 116 patients, 59 received preoperative HDR-VOBT (a dose of 8 Gy at 0.5 cm from the tumor surface), and 57 received traditional vaginal packing with gauze alone, followed by radical hysterectomy and lymphadenectomy. Analysis of the clinical parameters was performed using the chi-square test. The outcome measures were the 3- and 5-year survival and the complication rate.
From the 116 patients, 25 had stage IB1, 49 had stage IB2, and 42 had stage IIA1 cervical cancer. Nas a better hemostatic effect than vaginal packing with gauze, with no additional complications and no need for adjuvant treatment after the operation.
The aim of this study was to study patients diagnosed with ovary metastasis from colorectal carcinoma (OM-CRC) regarding their clinical manifestations, pathological characteristics and developments based on the results of computed tomography (CT).
Research was conducted with OM-CRC admissions to our facility during the period of January, 2000 to January, 2020, with examinations of both patients and imaging statistics.
A group of 21 female participants with a median age of 54-year-old, ranging from 48 to 68 years old, were investigated. In the univariate analyses, synchronous metastasis and R2 resection (macroscopic residual lesions) during cytoreductive surgery (CRS) were shown to be negatively associated with the OM-CRC prognosis. Results of the multivariate analysis showed that R2 resection during CRS was the only independent predictor of the overall survival (OS) rate as well as the progression-free survival (PFS) rate. OM-CRC cases frequently exhibited a well-defined borderline (90.9%), predominantly cystic ovarian mass (90.9%), heterogeneous enhancement (72.3%), moderate enhancement (63.6%), peritoneal implantation (81.8%) and an absence of lymphadenopathy (90.9%).
It is important to develop a thorough comprehension of the pathological characteristics as well as developments of OM-CRC, in order to understand its diagnosis, develop useful therapeutic interventions, and predict accurate prognosis. Nevertheless, OM-CRC is an uncommon malignant ovarian tumor and a good prognosis requires a CRS without macroscopic residual lesions, as a significant finding of our study.
It is important to develop a thorough comprehension of the pathological characteristics as well as developments of OM-CRC, in order to understand its diagnosis, develop useful therapeutic interventions, and predict accurate prognosis. Nevertheless, OM-CRC is an uncommon malignant ovarian tumor and a good prognosis requires a CRS without macroscopic residual lesions, as a significant finding of our study.
The study aims to retrospectively evaluate disparate reconstructive choices for defects in patients with primary hypopharyngeal carcinoma undergoing tumor resection.
One hundred and twenty-five patients of primary hypopharyngeal carcinamo conducted with hypopharynx reconstruction for restoring the defects causing by tumor ablation from 2003 to 2016 at Eye & ENT Hospital of Fudan University were reviewed. Most of them were treated with the following three techniques (n=115) gastric pull-up (GPU), the pedicled pectoralis major myocutaneous flap (PMMF) and the radial forearm free flap (RFFF). GPU was used for those with cervical esophagus invasion requiring esophageal resection in our institution. We focus on the postoperative complication morbidity, function of deglutition 1 month after hospital discharge and disease-free survival (DFS) outcomes.
One month after operation, 56 of 65 (86.2%) achieved solid or semisolid diet with normal swallowing function for GPU, 8 of 12 (66.7%) for RFFF and 20 of 33 (60.
