Robbsherman2401

The study was carried out on 27 healthy primiparous Holstein heifers (620 ± 50 kg) kept in a commercial dairy herd. The animals were divided into two groups taking into account the body condition score (BCS) index BCS 3.5 n = 15. The study period started one month before calving (BC), and ran until one month after calving (AC). Phorbol 12-myristate 13-acetate clinical trial Venous blood samples were collected 1 month and 1 week BC, and 1 week and 1 month AC. This study had two objectives (i) to assess whether a higher or lower BCS affected total milk production and its quality; (ii) to assess changes in the internal fluid (venous pH; partial pressure of CO2, ppCO2; bicarbonate; total CO2, TCO2; base excess, BE; electrolytes Na+, K+, Cl-; and anion gap, AG) that occur during this phase depending on the BCS. We can conclude that the BCS at calving does not affect the productive status during lactation, both in terms of the quantity and quality of milk produced. The excess of crude protein (CP) added through the ration in the lactation phase can trigger a tendency to an alkalotic state, in this case compensated by respiratory buffering mechanisms, as reflected by the TCO2. The changes in electrolytes are a reflection of the movement of free water for milk production, where a balance between measurable anions and cations is observed.A total of 64 piglets were used in a 35-day study to evaluate whether creep feeding piglets on the sow or super-dosing phytase to piglets post-weaning can be used as a tool to reduce stress and support adaption to weaning. Treatments consisted of creep or no creep feed being offered pre-weaning and with or without phytase supplementation at 2000 FTU/kg post-weaning. Blood samples were collected from eight piglets per treatment on days 0 (weaning), 7 and 21 post-weaning to determine plasma cortisol and myo-inositol concentrations. Four piglets per treatment (n = 16) were administered Heidelberg pH capsules 1 week prior to weaning, on the day of weaning, as well as 7 days and 21 days post-weaning, with readings monitored over a 3 h period. In the first week post-weaning, creep-fed piglets had higher daily gains (0.23 vs. 0.14 kg/d, p less then 0.05) and a lower feed conversion ratio (FCR, 0.99 vs. 1.35, p less then 0.01), compared to non-creep-fed pigs. At 21 days post-weaning, irrespective of creep feed, poved gastric conditions for digestion, phytate degradation and myo-inositol provision in piglets.The PI3K/Akt/PTEN axis is one of the most important signaling pathways in tumorigenesis. Recently, mutation of PIK3CA has been highlighted due to the similarities of mutational hotspots in both dogs and humans. PIK3CA H1047R (c.3140A > G) has been discovered as the most common mutational hot spot in canine mammary tumor in recent studies, while the feature of PIK3CA-mutated canine mammary tumor is obscure.

A total of 83 mammary samples classified as normal (

= 13), adenoma (

= 25), low-grade carcinoma (

= 21), and high-grade carcinoma (

= 24) were included in this study. Genomic DNA from each sample was extracted, amplified by conventional PCR, and analyzed through Sanger sequencing. Analysis for the expression of PIK3CA, Akt, p-Akt, and PTEN was performed by immunohistochemistry, and of Akt2 by RNA in situ hybridization.

PIK3CA H1047R mutation was detected in 14.3% (10/70) of tumor samples. Dysregulation of p-Akt, Akt2, and PTEN was observed in mammary tumor samples, but only PTEN dysregulation was associated with PIK3CA H1047R mutation.

The present study showed that dysregulation of components in the PI3K/Akt/PTEN pathway is a feature of canine mammary tumors, but this dysregulation is not directly correlated to the PIK3CA H1047R mutation except for PTEN expression.

The present study showed that dysregulation of components in the PI3K/Akt/PTEN pathway is a feature of canine mammary tumors, but this dysregulation is not directly correlated to the PIK3CA H1047R mutation except for PTEN expression.Pasture-borne parasites adversely affect bovine health and productivity worldwide. In Europe, gastrointestinal nematodes, especially Ostertagia ostertagi, the liver fluke Fasciola hepatica and the lungworm Dictyocaulus viviparus represent the most important parasites of dairy cattle. The present study assessed exposure towards these parasites among 646 cattle herds in three parts of Germany during 2017-2019 via antibody detection in bulk tank milk (BTM). Overall, O. ostertagi levels indicative of production losses were detected in 41.2% (266/646; 95% confidence interval (CI) 37.4-45.1%) of BTM samples, while F. hepatica seroprevalence amounted to 14.9% (96/646; 95% CI 12.2-17.9%). Only 2.3% (15/646; 95% CI 1.4-3.9%) of samples were D. viviparus antibody-positive. Significantly lower O. ostertagi as well as F. hepatica seroprevalence was detected in dual-purpose breeds compared to high-performance breeds from the same region. Management factors related to parasite exposure included access to fresh grass and hay, silage quality and anthelmintic treatment. Furthermore, F. hepatica and O. ostertagi seropositivity was significantly associated with suboptimal herd-level body condition. Interestingly, the relationship between seropositivity and productivity differed between breed types. Negative impacts on milk yield were detected only in high-performance breeds, while O. ostertagi seropositivity was associated with a lower milk fat content in dual-purpose herds.Small ruminants in northwestern Spain are frequently managed in mixed flocks. Sheep-goat joint management has not been considered a risk factor for parasite infection, so the main objective of this study was to establish if mixed management with goats supposes a risk factor for parasitic infections in ovine flocks. Two thousand and ninety-three sheep were sampled from 74 commercial meat ovine flocks for diagnostic of the main parasites. Goat contact was a risk factor for sheep to be infected by protostrongylids, Dictyocaulus filaria, gastrointestinal nematodes and Eimeria spp. In contrast, Moniezia, Fasciola hepatica, Dicrocoelium dendriticum and Paramphistomidae prevalences were not influenced. Sheep-goat mixed management can be considered as a risk factor, since goats would act as a source of pasture contamination for interspecific parasites (protostrongylids, Dictyocaulus filaria and gastrointestinal nematodes). In relation to host-specific parasites, such as Eimeria spp., goats cannot be considered as a source for sheep, but competition for food and spaces between both small ungulates can suppose a reduced grazing area to sheep, provoking high environmental contamination and stress that facilitate their infection. link2 Future epidemiological studies for parasitic infections in small ruminants should consider sheep-goat mixed management as a possible risk factor to be included in multivariate analyses.Being in a confined environment causes chronic stress in gestating sows, which is detrimental for sow health, welfare and, consequently, offspring physiology. This study assessed the health and welfare of gestating sows housed in a group housing system compared to individual gestation stalls. After pregnancy was confirmed, experimental sows were divided randomly into two groups the group housing system (GS), with the electronic sow feeding (ESF) system; or individual stall (IS). The behavior of sows housed in the GS or IS was then compared; throughout pregnancy, GS sows displayed more exploratory behavior, less vacuum chewing, and less sitting behavior (p less then 0.05). IS sows showed higher stress hormone levels than GS sows. In particular, at 41 days of gestation, the concentration of the adrenocorticotropic hormone (ACTH) and adrenaline (A) in IS sows was significantly higher than that of GS sows, and the A level of IS sows remained significantly higher at 71 days of gestation (p less then 0.01). The lipopolysaccharide (LPS) test was carried out in the weaned piglets of the studied sows. Compared with the offspring of gestating sows housed in GS (PG) or IS (PS), PG experienced a shorter period of high temperature and showed a quicker return to the normal state (p less then 0.05). Additionally, their lower levels of stress hormone (p less then 0.01) suggest that PG did not suffer from as much stress as PS. These findings suggested that gestating sows housed in GS were more able to carry out their natural behaviors and, therefore, had lower levels of stress and improved welfare. In addition, PG also showed better disease resistance and resilience. These results will provide a research basis for the welfare and breeding of gestating sows.The evaluation of zoo animals' personalities can likely lead to a range of benefits, including improving breeding success, creating stable social groups, and designing and developing environmental enrichment programmes. The goal of this study was to use caretakers scores to evaluate personality in bottlenose dolphins and to assess the reliability of scores within each rater and among raters from each centre. To this end, 24 caretakers from 3 countries (Spain, France, and Argentina), including a total of 5 dolphinariums and 6 groups of dolphins, used a questionnaire based on the Five-Factor Model of Personality to score bottlenose dolphins on a number of personality traits in three different contexts. link3 Each caretaker evaluated the animals under their care twice, ensuring that raters did not share thoughts nor impressions with other raters. Our findings showed a good degree of agreement between each rater's scores and a fair degree of agreement among scores of raters from the same centre. We also identified which raters and centres had significant mean score differences and detected that 4 out of 24 raters from two different centres showed such differences systematically. The evaluation of raters' reliability and the identification of particular inconsistent raters and centres is critical to make more appropriate and realistic management decisions that, in turn, directly impact animals' welfare.Suncus etruscus is the smallest living mammal on Earth by mass. Most adults weigh 1.8-3 g with a body length of 35-48 mm. Catching it in small mammal traps in nature is extremely difficult due to its minute size, and therefore special trapping methods must be used. We had the unique opportunity of studying, for the first time, the helminth parasites of 166 individuals of S. etruscus, part of the largest collection in the world, which belonged to the French scientist Dr Roger Fons (1942-2016). A total of 150 individuals were captured in the Banyuls-Cerbère area (France) and 16 in the island of Corsica (France). We found seven helminth species, specifically, the cestodes Joyeuxiella pasqualei larvae, Mesocestoides sp. larvae, Staphylocystis claudevaucheri, S. banyulsensis, S. cerberensis, and Pseudhymenolepis sp., and the nematodes Aonchotheca sp. and Nematoda gen. sp. larvae. Neither trematodes nor acanthocephalans were detected. We provide prevalences, infracommunity compositions, and helminth associations. The adult helminth community of S. etruscus seems to be highly specific, i.e., oioxenous, and linked to its insectivore diet. Due to its small size, S. etruscus has undergone numerous physiological adaptations that have probably influenced its helminth spectrum as well as its helminth specificity.