Roachserrano1992

These findings differed from those for ICAM-1, where gene expression was increased in asthma and less consistent differences were observed related to gender, race, and use of ICS. CONCLUSION Higher expression of ACE2 and TMPRSS2 in males, African Americans, and patients with diabetes mellitus provides rationale for monitoring these asthma subgroups for poor COVID19 outcomes. The lower expression of ACE2 and TMPRSS2 with ICS use warrants prospective study of ICS use as a predictor of decreased susceptibility to SARS-CoV-2 infection and decreased COVID19 morbidity. This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (http//creativecommons.org/licenses/by-nc-nd/4.0/).A growing body of evidence demonstrates that long non-coding RNAs (lncRNAs) are correlated with various cancers. LncRNA LINP1 has been proven as a key factor of malignant tumor development. However, it is still unclear about the role of LINP1 in cervical cancer (CC). In our research, a prominent increase of LINP1 was found in CC tissues in comparison with adjacent normal tissues. What's more, LINP1 expression was identified to be upregulated in CC cells compared with human cervical epithelial cell lines (HUCEC). Surprisingly, the downregulation of LINP1 markedly alleviated the proliferation and promoted the apoptosis of cells in CC. Besides, LINP1 significantly decreased CC tumor growth in vivo. Further, we observed LINP1 recruited EZH2, LSD1 and DNMT1 to reduce the expression of KLF2 and PRSS8. RT-qPCR showed silence of LINP1 elevated the expression of KLF2 and PRSS8 in CC cells. Loss-of-function assays disclosed upregulation of KLF2 and PRSS8 inhibited cell proliferation and boosted cell apoptosis in CC. Besides, inhibition of KLF2 and PRSS8 could reversed the inhibitory effect on cell proliferation resulted from LINP1 suppression. MK-0991 In short, LINP1 aggravated the progression of CC by suppressing KLF2 and PRSS8, which might provide a promising target for CC treatment.Human noroviruses cause significant morbidity and mortality worldwide but lack approved antivirals or vaccines to treat or prevent infections. The recent development of two cell culture systems in human transformed B cells (BJABs) and non-transformed human intestinal enteroids cultures overcomes a main limitation in identifying molecules with anti-norovirus activities. Lactoferrin is an iron-binding glycoprotein found in the milk of most mammals with broad spectrum antimicrobial activities, including against the related murine norovirus in cell culture. Its ingestion also reduced the incidence of infectious gastroenteritis in Japanese clinical trial participants. Since human noroviruses were the most common cause of gastroenteritis in Japan during the clinical trial period, we sought to determine whether lactoferrin can inhibit human norovirus infection. Our study demonstrates that lactoferrin reduces human norovirus infection in the B cell culture model. The mechanism of antiviral action is likely indirect and may involve induction of innate interferon responses. Therefore, future studies are warranted to test the antiviral efficacy of lactoferrin against human norovirus infection in patients.This randomized, double-blinded, placebo-controlled study aimed to investigate the effect of strength training (ST) combined with vitamin C and E supplementation on perceived and performance fatigability in breast cancer survivors (BCS). Twenty-five BCS were randomly assigned to one of two groups vitamins (VIT; n = 12; 51.0 ± 9.0 years); or placebo (PLA; n = 13; 48.2 ± 8.3 years). Both groups performed a 10-week ST protocol, twice a week. VIT was supplemented with vitamins C (500mg/day) and E (180mg/day) and PLA with polydextrose (1g/day), once a day after breakfast. At the beginning and at the end of training period, perceived fatigability was assessed using MFI-20 (general fatigue and physical fatigue). Performance fatigability was assessed during 30 maximal isokinetic knee extension at 120º/s-1. General fatigue reduced similarly in VIT (p = 0.004) and PLA (p = 0.011). Physical fatigue reduced similarly in both, VIT (p = 0.011) and PLA (p = 0.001). Performance fatigability also decreased similarly in VIT (p = 0.026) and PLA (p 0.05). In summary, antioxidants supplementation does not add any positive synergistic effect to ST on improving perceived or performance fatigability in BCS. Novelty -Strength training with maximal repetitions reduces perceived and performance fatigability of BCS. -Vitamins C and E supplementation does no add any positive synergistic effect to ST on reducing fatigability in BCS.RATIONALE Medical interventions that prolong life without achieving an effect that the patient can appreciate as a benefit are often considered futile or inappropriate by health care providers. In recent years, a multi-center guideline has been released with recommendations on how to resolve conflicts between families and clinicians in these situations and to increase public engagement. Although lay-people are acknowledged as important stakeholders, their perceptions and understanding of the terms "potentially inappropriate" or "futile" treatment have received little formal evaluation. OBJECTIVE To evaluate the community perspective about the meaning of futile treatment. METHODS Six focus groups (two groups each of ages 75) were convened to explore what constitutes futile treatment and who should decide in situations of conflict between doctors and families. Focus group discussions were analyzed using grounded theory. RESULTS There were thirty-nine participants aged 18 or older with at least one previous hospitalization (personal or by immediate relative). When asked to describe "futile," or "inappropriate" treatment, community members found the concept difficult to understand and the terminology inadequate, though when presented with a case describing inappropriate treatment, most participants recognized it as the provision of inappropriate treatment. Several themes emerged regarding participant difficulty with the concept, including inadequate physician-patient communication, lack of public emphasis on end-of-life issues, skepticism that medical treatment can be completely inappropriate, and doubts and fears that medical futility could undermine patient/family autonomy. Participants also firmly believed that in situations of conflict, families should be the ultimate decision-makers. CONCLUSIONS Public engagement in policy development and discourse around medical futility will first require intense education to familiarize the lay-public about use of inappropriate treatment at the end of life.