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The phosphoprotein AHNAK is a large, ubiquitously expressed scaffolding protein involved in mediating a host of protein-protein interactions. This enables AHNAK to participate in various multi-protein complexes thereby orchestrating a range of diverse biological processes, including tumour suppression, immune regulation and cell architecture maintenance. Selleckchem Sivelestat A less studied but nonetheless equally important function occurs in calcium homeostasis. It does so by largely interacting with the L-type voltage-gated calcium channel (LVGCC) present in the plasma membrane of excitable cells such as muscles and neurons. Several studies have characterized the underlying basis of AHNAK's functional role in calcium channel modulation, which has led to a greater understanding of this cellular process and its associated pathologies. In this article we review and examine recent advances relating to the physiological aspects of AHNAK in calcium regulation. Specifically, we will provide a broad overview of AHNAK including its structural makeup and its interaction with several isoforms of LVGCC, and how these molecular interactions regulate calcium modulation across various tissues and their implication in muscle and neuronal function.Several proteins containing C2 domains have been identified as Ca2+ sensors for neurotransmitter release. In several cases, multiple C2 domain containing proteins function together to sustain evoked synchronous and asynchronous release as well as Ca2+-dependent forms of spontaneous release. Most recent publication by Li and colleagues have identified a novel Ca2+ sensor at the C. elegans neuromuscular junction [8] that complements the fast Ca2+ sensor synaptotagmin-1 in mediating a slower form of evoked release. Here, we discuss these results as well as earlier work suggesting an evolutionarily conserved diversity of Ca2+ sensors mediating distinct forms of neurotransmitter release.This study investigated the impacts of different bulking agents (i.e. garden waste, cornstalks, and spent mushroom substrates) on bacterial structure and functions for gaseous emissions during kitchen waste composting. High-throughput sequencing was integrated with functional Annotation of Prokaryotic Taxa (FAPROTAX) to decipher the bacterial structure and functions. Results show that adding cornstalks constructed a more complex and mutualistic bacterial network to enhance organic biodegradation. This scenario, however, aggravated the emission of ammonia and hydrogen sulphide with the enrichment of the genus Bacillus and Desulfitibacter at the thermophilic stage of composting to facilitate ammonification and sulphur-related respiration, respectively. By contrast, spent mushroom substrates facilitated the proliferation of the genus Pseudomonas to promote nitrate reduction at the cooling stage, leading to considerable emission of nitrous oxide. Compared to these two agents, garden waste contained less easily biodegradable substances to limit bacterial mutualism, thereby reducing gaseous emissions in composting.Microalgae is considered as a renewable and sustainable biomass to produce bioenergy and other high-value products. Besides, the cultivation of microalgae does not need any fertile land and it provides opportunities for climate change mitigation by sequestering atmospheric carbon-dioxide (CO2), facilitating nutrient recovery from wastewater and regulating industrial pollutions/emissions. Algal biomass harvested from different technologies are unique in their physio-chemical properties that require critical understanding prior to value-addition or bioenergy recovery. In this review, we elaborate the importance of cell wall weakening followed by pretreatment as a key process step and strategy to reduce the energy cost of converting algal biomass into bioenergy. From the energy-calculations, it was measured that the cell wall weakening significantly improves the net-energy ratio from 0.68 to 1.02. This approach could be integrated with any pre-treatment options, while it reduces the time of pre-treatment and costs of energy/chemicals required for hydrolysis of algal biomass.Biorefining of lignocellulosic biomass is a relatively new concept but it has strong potential to develop and partially replace the fossil derived fuels and myriad of value products to subsequently reduce the greenhouse gas emissions. However, the energy and cost intensive process of releasing the entrapped fermentable sugars is a major challenge for its commercialization. Various factors playing a detrimental role during enzymatic hydrolysis of biomass are inherent recalcitrance of lignocellulosic biomass, expensive enzymes, sub-optimal enzyme composition, lack of synergistic activity and enzyme inhibition caused by various inhibitors. The current study investigated the mechanism of enzyme inhibition during lignocellulosic biomass saccharification especially at high solid loadings. These inhibition factors are categorized into physio-chemical factors, water-soluble and -insoluble enzyme inhibitors, oligomers and enzyme-lignin binding. Furthermore, different approaches are proposed to alleviate the challenges and improve the enzymatic hydrolysis efficiency such as supplementation with surfactants, synergistic catalytic/non-catalytic proteins, and bioprocess modifications.Short-term composting of raw materials for preparing oyster mushroom cultivation media is widely used in China, and its microbial mechanism needs to be further studied. 11-days' peach sawdust-based composting was performed to evaluate material conversion and microbial succession using physicochemical analysis and 16S rRNA and ITS sequencing. Composting bacteria demonstrated much higher abundance than fungi. Firmicutes, Actinobacteriota, and Proteobacteria were the dominant bacterial phyla, while most of fungal species belonged to Ascomycota. Moisture was the key factor at the beginning, while total nitrogen, temperature, and lignin became main influencing factors for composting maturity. Actinobacteriota, Firmicutes, and Proteobacteria of bacterial phyla, Eurotiomycetes and Sordariomycetes of fungal classes involved in lignocellulosic degradation. Bacterial function prediction analysis showed that carbohydrate metabolism and amino acid metabolism were the main metabolic pathways. These results confer a better understanding of material and microbial succession during short-term composting and also provide valuable utilization in mushroom industry.