Refsgaardgregory8199

nvestigate this, MCs were loaded with BaSO4, and orally administered to mice. Analysis with X-ray and CT showed a transit time of approximately 1-1.5 h from the stomach to the cecum, corresponding to the standard transit time in mice, and an extremely narrow absorption window. This indicates that mice is not a suitable animal model for evaluation of MCs. These data should be taken into consideration in future in vivo trials with this and similar technologies, where larger animals might be a necessity for proof-of-concept studies.With the rapid development of biopharmaceuticals and the outbreak of COVID-19, the world has ushered in a frenzy to develop gene therapy. Therefore, therapeutic genes have received enormous attention. However, due to the extreme instability and low intracellular gene expression of naked genes, specific vectors are required. Viral vectors are widely used attributed to their high transfection efficiency. However, due to the safety concerns of viral vectors, nanotechnology-based non-viral vectors have attracted extensive investigation. Still, issues of low transfection efficiency and poor tissue targeting of non-viral vectors need to be addressed. Especially, pulmonary gene delivery has obvious advantages for the treatment of inherited lung diseases, lung cancer, and viral pneumonia, which can not only enhance lung targeting and but also reduce enzymatic degradation. For systemic diseases therapy, pulmonary gene delivery can enhance vaccine efficacy via inducing not only cellular, humoral immunity but also mucosal immunity. This review provides a comprehensive overview of nanocarriers as non-viral vectors of therapeutic genes for enhanced pulmonary delivery. First of all, the characteristics and therapeutic mechanism of DNA, mRNA, and siRNA are provided. Thereafter, the advantages and challenges of pulmonary gene delivery in exerting local and systemic effects are discussed. Then, the inhalation dosage forms for nanoparticle-based drug delivery systems are introduced. Moreover, a series of materials used as nanocarriers for pulmonary gene delivery are presented, and the endosomal escape mechanisms of nanocarriers based on different materials are explored. The application of various non-viral vectors for pulmonary gene delivery are summarized in detail, with the perspectives of nano-vectors for pulmonary gene delivery.Sepsis, a consequence of an imbalanced immune response to infection, is currently one of the leading causes of death globally. Despite advances in the discoveries of potential targets and nanotechnology, sepsis still lacks effective drug delivery systems for optimal treatment. Stimuli-responsive and biomimetic nano delivery systems, specifically, are emerging as advanced bio-inspired nanocarriers for enhancing the treatment of sepsis. Herein, we present a critical review of different stimuli-responsive systems, including pH-; enzyme-; ROS- and toxin-responsive nanocarriers, reported in the delivery of therapeutics for sepsis. Biomimetic nanocarriers, utilizing natural pathways in the inflammatory cascade to optimize sepsis therapy, are also reviewed, in addition to smart, multifunctional vehicles. The review highlights the nanomaterials designed for constructing these systems; their physicochemical properties; the mechanisms of drug release; and their potential for enhancing the therapeutic efficacy of their cargo. Current challenges are identified and future avenues for research into the optimization of bio-inspired nano delivery systems for sepsis are also proposed. This review confirms the potential of stimuli-responsive and biomimetic nanocarriers for enhanced therapy against sepsis and related complications.This study highlights the microfibrillation potential of three deep eutectic solvents (DES) composed of betaine hydrochloride-urea, choline chloride-urea and choline chloride-monoethanolamine. Cellulose fibres (eucalyptus and cotton) were first treated in DES (100 °C for 4 h) and then ground with an ultra-fine grinder to produce microfibrillated cellulose (MFC). DES pre-treatment especially betaine hydrochloride-urea system has significantly improved the microfibrillation process with reduced energy consumption comparable to that of enzymatic treatment (reference pre-treatment). Long and thin microfibril bundles (widths around 50 nm) and individualised microfibrils (widths between 5 and 10 nm) were obtained. MFC gels and nanopapers were characterised using several techniques. Nanopapers produced from DES treated MFC showed good mechanical properties with Young's modulus higher than 10 GPa. In addition, they exhibited higher quality index (between 73 and 76) than those produced from enzymatic hydrolysis (quality index around 68). DES pre-treatment is a promising way to produce MFC with high aspect ratio.To investigate the effect of granular activated carbon (GAC) adsorption and size of microbial aggregates in inoculum on stimulating direct interspecies electron transfer (DIET) during anaerobic digestion of fat, oil, and grease (FOG), seed sludge was divided into two inocula (big (>0.85 mm)/small (0.15-0.85 mm)) for FOG digestion with/without GAC. More long-chain fatty acids (LCFAs) were adsorbed on GAC in the reactor with small aggregates than that with big aggregates, corresponding to 57 % and 10 % decreased methane production, respectively. Molnupiravir ic50 Adsorption of unsaturated LCFAs (e.g., oleic acid) on GAC was found to reduce LCFA bioavailability, hinder DIET via GAC, and change community structure. Compared to pre-adsorption of oleic acid on GAC, pre-attachment of microbes on GAC resulted in 5.6-fold higher methane yield for oleic acid digestion. Together, competition of LCFA adsorption between GAC and microbial aggregates is essential for enhanced methane recovery from FOG digestion via GAC-induced DIET.To preserve the water resources, this study has analyzed the ecotoxicity and antibiotic resistance genes (ARGs) induction capacity of sulfadiazine degradation intermediates resulting from persulfate activation oxidation enhanced by ultraviolet, ultrasound and microwave. The five degradation pathways caused by the contribution discrepancy of electron transfer and singlet oxygen (1O2) and variations in the ecotoxicity of different degradation products were analyzed. Microcosm experiment exhibited that the microbial community in actual water changed significantly with SDZ and degradation intermediates, in which the dominant genera were Aeromonas, Cupriavidus, Elizabethkingia and Achromobacter. Except for the selective pressure on bacteria, the degradation intermediates also exert a certain degree or even stronger induction on sulfonamide ARGs (sul4, sul1 and sul2) than SDZ. Furthermore, the potential hosts for sulfonamide ARGs were revealed by network analysis. These results provide a better understanding of antibiotics degradation mechanism and ARGs occurrence, which is useful for controlling the spread of ARGs.

This study aimed to determine the prevalence and factors associated with frailty in older hospitalized patients.

The point-prevalence study was completed on 263 patients aged 65 and over hospitalized in internal medicine and surgical clinics at a tertiary hospital in Türkiye. Data were collected between July 19th and July 22nd, 2021. A comprehensive geriatric assessment was performed on the participants. The Edmonton Frailty Scale (EFS) and FRAIL scale were used for frailty assessment.

The mean age of the individuals was 72.40±6.42, 51.7% were female, and 63.9% were hospitalized in internal medicine and surgical units. The prevalence of frailty was 57.4% according to the FRAIL scale and 46.8% according to EFS. Factors affecting frailty were gender (OR 3.36, 95% CI 1.48-7.64), comorbidity (OR 1.29, 95% CI 1.01-1.64), polypharmacy (OR 0.33, 95% CI 0.13-0.80), history of falling in the last year (OR 3.54, 95% CI 1.34-9.35), incontinence (OR 5.93, 95% CI 2.47-14.27), and functional dependency (ADL, OR 0.65, 95% CI 0.46-0.92; IADL, OR 0.59, 95% CI 0.46-0.76). This model correctly predicted the participants' frailty at 70.5%.

The importance of frailty, which affects one out of every two hospitalized older persons, to the health care system should not be overlooked. Considering the increasing trend of the aging person population, national and global plans should be made to prevent and manage frailty.

The importance of frailty, which affects one out of every two hospitalized older persons, to the health care system should not be overlooked. Considering the increasing trend of the aging person population, national and global plans should be made to prevent and manage frailty.

Fenestrated/branched endovascular aortic repair (F/BEVAR) in patients with occluded iliac arteries is challenging due to limited access for branch vessel catheterization and increased risk for leg and spinal ischemic complications. The aim of this study was to analyze technical strategies and outcomes of F/BEVAR in patients with unilateral iliofemoral occlusive disease (UIOD).

We performed a retrospective review of all consecutive patients treated by F/BEVAR in two institutions (2003-2021). Patients with UIOD were included in the analysis. All patients had one patent iliac artery which was used for advancement of the fenestrated-branch component. Preloaded catheter/guidewire systems or steerable sheaths were used as adjuncts to facilitate catheterization. Primary endpoints were technical success, mortality, major adverse events (MAEs stroke, spinal cord injury, dialysis/GFR decline >50%, bowel ischemia, myocardial infarction or respiratory failure), primary iliac patency and freedom from reintervention2 months (0-85). Two patients (13%) required three reinterventions. One patient required proximal stent-graft extension for an acute type B dissection (3 months) and another required iliac extension for type Ib endoleak of an AUI (21 months) and thrombolysis of that extension (50 months). At last follow-up all patients had primary graft patency except one with secondary graft patency without new claudication. One patient had a single renal artery stent occlusion at follow-up with no re-intervention. Overall survival was 60%, without aortic-related deaths.

Although challenging, F/BEVAR with unilateral femoral/brachial approach is feasible in patients with occluded iliac limbs, with an important rate of ischemic complications but satisfactory outcomes.

Although challenging, F/BEVAR with unilateral femoral/brachial approach is feasible in patients with occluded iliac limbs, with an important rate of ischemic complications but satisfactory outcomes.Caspases, a family of cysteine proteases is a renowned regulator of apoptosis. Members of this family are responsible for the proteolytic dismantling of numerous cellular structures. Apart from apoptosis, caspases remarkably contribute to a diverse range of molecular processes. Being the imperative members of several cellular cascades their abnormal activation/deactivation has severe implications and also leads to various diseased conditions. Similar aberrant activation of caspases is one of the several causes of neuropathologies associated with Alzheimer's disease (AD), a form of dementia severely affecting neuropsychiatric and cognitive functions. Emerging studies are providing deeper insights into the mechanisms of caspase action in the progression of AD. Current article is an attempt to review these studies and present the action mechanisms of different mammalian caspases in the advancement of AD associated neuropathologies.