Petersonellegaard5801
ns may disturb the control of sleep-wake homeostasis. These findings suggest that the subcortical system is a primary mechanism associated with sleep disturbances in the early stages of neurodegenerative diseases.
In this cohort study, subcortical wake-promoting neurons were significantly correlated with sleep phenotypes in patients with AD and PSP, suggesting that the loss of wake-promoting neurons among patients with neurodegenerative conditions may disturb the control of sleep-wake homeostasis. These findings suggest that the subcortical system is a primary mechanism associated with sleep disturbances in the early stages of neurodegenerative diseases.
To analyze the microbiological and microstructural part of indwelling urinary catheters and their association with urinary tract infection prevention.
This is a cross-sectional study, from June to December 2020, in which 42 indwelling urinary catheter tips and sterile urine samples were collected for analysis of crystals in optical microscopy and biofilms in scanning electron microscopy. Culture analysis and specification of the type of bacteria were performed.
It was found that 35.71% of the samples had mature biofilm adhered to the catheter tip. Biofilms of Proteus mirabilis, Enterococcus faecalis, Staphylococcus epidermidis, Enterococcus faecium and Enterobacter cloacae stood out. The presence of magnesium-ammonium-phosphate crystal was associated with the presence of urinary tract infection and with Proteus mirabilis. There was a significant association (p = 0.001) between the use of prophylactic antibiotics versus urine culture >105 CFU/mL.
The analyzes contributed to clinical practice, as it reinforces the development of effective and monitored strategies on cultures and urinary tract infection prevention associated with indwelling urinary catheters.
The analyzes contributed to clinical practice, as it reinforces the development of effective and monitored strategies on cultures and urinary tract infection prevention associated with indwelling urinary catheters.
To describe and discuss the participation of adolescents from a quilombola community in the transformation of the comic "Possible Story" ("Uma História Possível"), from the Comic on alcohol, into an educational game.
Implementation of the creative and sensitive method of art-based research, with adolescents from a quilombola community in the state of Espírito Santo, for the development of a board game.
The democratic and interactive space favored the problematization of images and narratives about alcohol consumption mediated in the comic book. The group selected scenes, reordered the story, devised questions and riddles, formulated true and false assertions in a 17-card composition of the board game. The potential of the game as a content mediating tool to promote learning, reinforcement, and fixation of scientific content was evaluated.
The active and dynamic participation of adolescents took place from conception to evaluation of the board game, encouraging them to reflect on a community context of cultural permissiveness of alcohol use.
The active and dynamic participation of adolescents took place from conception to evaluation of the board game, encouraging them to reflect on a community context of cultural permissiveness of alcohol use.
To assess the understandings of a pedagogical intervention on the Brazilian National Policy of Permanent Health Education targeted at secondary technical and vocational nursing students.
Applied, pedagogical intervention study conducted with twenty-three students of a secondary technical nursing course; questionnaires, focal group, and thematic content analysis were employed.
Intervention, collectively built by manager, nursing teachers, and researchers, is assessed to have led to a problematization of the concepts of education and continuing and permanent education. The following thematic categories emerged from the analysis Prior knowledge of students and understandings of the classroom intervention; Relation between permanent education and educational welcome in health units; Ethics concerns and the articulation of care practice and theory; and Work process and approximations to permanent health education.
The pedagogical intervention is assessed to have favored the critical reflection of the aspiring nursing technicians on permanent health education and the need for a collaborative pedagogical planning for aligning the health team's work process.
The pedagogical intervention is assessed to have favored the critical reflection of the aspiring nursing technicians on permanent health education and the need for a collaborative pedagogical planning for aligning the health team's work process.Atrial fibrillation (AF) represents the most common type of sustained cardiac arrhythmia in humans and confers a significantly increased risk for thromboembolic stroke, congestive heart failure and premature death. Aggregating evidence emphasizes the predominant genetic defects underpinning AF and an increasing number of deleterious variations in more than 50 genes have been involved in the pathogenesis of AF. Nevertheless, the genetic basis underlying AF remains incompletely understood. In the current research, by whole-exome sequencing and Sanger sequencing analysis in a family with autosomal-dominant AF and congenital patent ductus arteriosus (PDA), a novel heterozygous variation in the PRRX1 gene encoding a homeobox transcription factor critical for cardiovascular development, NM_022716.4c.373G>T;p.(Glu125*), was identified to be in co-segregation with AF and PDA in the whole family. The truncating variation was not detected in 306 unrelated healthy individuals employed as controls. Quantitative biological measurements with a reporter gene analysis system revealed that the Glu125*-mutant PRRX1 protein failed to transactivate its downstream target genes SHOX2 and ISL1, two genes that have been causally linked to AF. Conclusively, the present study firstly links PRRX1 loss-of-function variation to AF and PDA, suggesting that AF and PDA share a common abnormal developmental basis in a proportion of cases.
To examine the effect of breastfeeding educational intervention given in the antenatal period on LATCH and breastfeeding self-efficacy scores.
A total of 80 pregnant who met the research criteria were randomly assigned to intervention (n = 40) or control (n = 40) groups. Pregnant women received to the control group received only standard care while breastfeeding education was accepted to the intervention group along with standard care. Both groups were visited at their home, and the personal data form, the LATCH Breastfeeding Assessment Tool, and Breastfeeding Self-Efficacy Scale-Short Form (BSES-SF) were applied in the postpartum 1st week. End of the study, brochures prepared by the researcher were given to both groups.
The mean breastfeeding self-efficacy and LATCH scores were higher in the intervention group compared to the control group. Breastfeeding success was found to increase as the maternal breastfeeding self-efficacy perception increased.
Breastfeeding education given in the antenatal period increased maternal breastfeeding self-efficacy perception and breastfeeding success in the postpartum 1st week period.Study is registered at ClinicalTrials.gov NCT04757324.
Breastfeeding education given in the antenatal period increased maternal breastfeeding self-efficacy perception and breastfeeding success in the postpartum 1st week period.Study is registered at ClinicalTrials.gov NCT04757324.Protein kinase can catalyze the phosphorylation of peptides/proteins, and it is closely associated with various human diseases such as cancer, immune deficiencies, and Alzheimer's disease. read more Sensitive monitoring of protein kinase activity is significant for biochemical research and drug discovery. Herein, we develop a phos-tag-based fluorescent biosensor for sensitive detection of cAMP-dependent protein kinase (PKA) activity in cancer cells. We design a peptide-DNA conjugate and a signal probe for PKA activity assay, and employ a biotinylated phos-tag (a selective phosphate-binding agent) to recognize and capture the phosphorylated peptide-DNA substrate. The peptide-DNA conjugates as the catalytic substrates can translate the peptide signal to a nucleotide signal for the initiation of the RNase HII-driven cycling signal amplification. The magnetic nanobeads (MBs) as the carriers can separate and enrich the phosphorylated peptide in complex matrices. Meanwhile, the combination of MBs with the phos-tag-mediated phosphate group recognition can effectively eliminate the interference from the complex matrix, and the introduction of single-molecule detection endows this assay with high sensitivity. This biosensor can achieve a detection limit of 1.98 × 10-8 U μL-1 and a wide dynamic range from 1 × 10-7 to 1 × 10-2 U μL-1. Moreover, this biosensor can be applied for the screening of PKA inhibitor and the measurement of cellular PKA activity, holding great potential in biomedical research and clinical diagnosis.The development of an elution-free solid-phase extraction (SPE) process is of special interest in sample pretreatment. Due to the phase-change merits at relatively low temperatures and easy dissolution in n-hexane, wax spheres show great potential in this field. However, the conventional wax spheres possess a low affinity towards the target analytes when they are used as SPE adsorbents. In this study, using octadecanoic acid as the functional monomer and wax as the matrix, molecularly imprinted wax (MIW) spheres were successfully prepared. The obtained MIW spheres displayed remarkable molecular recognition ability and high selectivity towards the template. Interestingly, the as-synthesized molecularly imprinted wax (MIW) could be dissolved in n-hexane or melted by heating for subsequent fluorescence and mass spectrum analysis without the target elution process. Moreover, the melted MIW exhibited high repeatability, sensitivity and specificity for solid-state fluorescence detection. We believe that the imprinting method presented in this study will open a new window in analytical chemistry.The liquid crystal dimer 1,7-bis-4-(4'-cyanobiphenyl)heptane (CB7CB) is known to exhibit a nematic-nematic phase transition, with the lower temperature phase identified as the twist-bend nematic (NTB) phase. Despite the achiral nature of the mesogen, the NTB phase demonstrates emergent chirality through the spontaneous formation of a helical structure. We present extensive molecular dynamics simulations of CB7CB using an all-atom force field. The NTB phase is observed in this model and, upon heating, shows phase transitions into the nematic (N) and isotropic phases. The simulated NTB phase returns a pitch of 8.35 nm and a conical tilt angle of 29°. Analysis of the bend angle between the mesogenic units reveals an average angle of 127°, which is invariant to the simulated phase. We have calculated distributions of the chirality order parameter, χ, for the ensemble of conformers in the NTB and N phases. These distributions elucidate that CB7CB is statistically achiral but can adopt chiral conformers with no preference for a specific handedness.
