Perezharris3412

The aim of the current study was to evaluate the probabilistic health risk and the concentration of 16 polycyclic aromatic hydrocarbons (PAHs) in commercial tea and coffee samples. For determining the mentioned contaminants in sixty-four samples, a reliable and sensitive technique was validated and developed. The technique is established on magnetic solid-phase extraction and gas chromatography-mass spectrometry analysis (MSPE/GC-MS). The maximum mean of ƩPAHs in coffee samples was 13.75 ± 2.90 μg kg-1, while the minimum mean ƩPAHs in tea samples was 4.77 ± 1.01 μg kg-1. The mean concentration of benzo(a)pyrene (BaP) in samples ranged from 0.64 to 2.07 μg kg-1 which was lower than that of standard levels (10 μg kg-1) established by the European Union (EU). The Monte Carlo simulation results showed that the actual target hazard quotient (THQ) for the adult and children was equal to 1.63E-04 and 1.67E-04, respectively; hence, non-carcinogenic health risk for consumers is negligible. The result of actual incremental lifetime cancer risk (ILCR) was lower than the limits of safe risk (1E-4), indicating no notable possibility of cancer risk due to the digestion of tea and coffee for children and adults. Therefore, it can be concluded that the amount of contamination of popular commercial coffee and tea available in the Iranian market with PAHs is often similar to that found in other countries and was lower than the standard of EU. Thus, the processing conditions of these products must be controlled to prevent the formation of PAHs due to the suspicion of carcinogenicity and mutation.In Guadeloupe, the use between 1972 and 1993 of chlordecone, an organochlorine insecticide, has permanently contaminated the island's soil, thus contaminating the food chain at its very beginning. There is today a strong societal requirement for an improved mapping of the contaminated zones. Given the extent of the areas to be covered, carrying out soil tests on each plot of the territory would be a long and expensive process. In this article, we explore a method of demarcating polluted areas. The approach adopted consists in carrying out, using surface water analyses, a hydrological delimitation that makes it possible to distinguish contaminated watersheds from uncontaminated ones. The selection of sampling points was based on the spatial analysis of the actual and potential contamination data existing at the beginning of the study. The approach was validated by soil analyses, after having compared the contamination data of the watersheds with the soil contamination data of the plots within them. The study thus made it possible to highlight new contaminated areas and also those at risk of contamination and to identify the plots to be targeted as a priority during future analysis campaigns by State services.Intensification of pig production in developing countries has resulted in increased number of animals per unit area leading to welfare and growth performance issues. This study was then performed to evaluate the effects of space allowance and dietary energy and amino acid content on growth performance and physiological parameters of piglets reared under tropical conditions. A total of 1280 piglets (castrated males and females) with initial body weight of 5.9 ± 0.4 kg were used. Animals were distributed in a 2 × 2 factorial arrangement composed by two space allowances (0.30 and 0.22 m2/animal) and two diets during 43 to 63 days of age (control and experimental diet with increased ME and AA content). The experimental period lasted 42 days subdivided into four phases according to the growth stage of the animals pre-initial I (21 to 27 days of age), pre-initial II (28 to 34 days), initial I (35 to 42 days), and initial II (43 to 63 days). During pre-initial I and II, and initial I phases, pigs housed at a space allowance of 0.30 and 22 m2/animal had similar (P > 0.05) feed intake, weight gain, and feed conversion. During initial phase II, space allowance did not affect (P > 0.05) feed intake nor weight gain of the animals. Whereas, pigs fed the experimental diet had greater weight gain when compared to the control group (530 vs. 515 g/day, P = 0.03). According to our results, reducing space allowance from 0.30 to 22 m2/animal during the nursery phase (21 to 63 days of age) had negligible effects on piglet feed intake and weight gain, whereas feeding piglets with diets containing higher energy and amino levels resulted in greater weight gain.The study evaluates the effect of three hormonal protocols on ovarian dynamics and progesterone (P4) secretion of buffalo (Bubalus bubalis). Twenty-nine pluriparous Murrah buffaloes were used. The protocols were as follows OVSYNCH (n = 10) 100 μg of gonadorelin (day 0), 500 μg of cloprostenol (day 7), and 100 μg of gonadorelin (day 9). CIDR+EB (intravaginal device (CIDR®) + estradiol benzoate; n = 10) CIDR plus 2 mg of EB (day 0), withdrew of CIDR, 500 μg of cloprostenol (day 7) and 1 mg of EB (day 8). CIDR+eCG (n = 9) CIDR plus 2 mg of EB (day 0), withdrew of CIDR, 500 μg of cloprostenol and 400 IU of eCG (day 7). Follicles were counted with an ultrasound and measured at 0, 24, and 54 h. The maximum follicle diameter and ovulation were evaluated at 70, 80, and 94 h after CIDR withdrew. Estrous was detected per 1 h three times daily. Blood samples were collected on days 0, 7, 10, 15, and 22 to determine P4 concentration. In CIDR+EB protocol, 50% of buffaloes presented estrous, at 69.6 h. All buffaloes ovulated. CIDR+eCG group had the shortest (69 h) ovulation time. No treatment differences for follicular population, maximum follicle diameter, and P4 concentration on days 7 and 10 (P > 0.05) were found. The P4 concentration in OVSYNCH and CIDR+eCG protocols were > 1 ng/ml, on days 15 and 22 (P less then  0.05). BMS-986365 There was no difference in ovarian activity; however, the P4 secretion was normal in the OVSYNCH and CIDR+eCG protocols compared to the CIDR+EB protocol.MicroRNAs (miRNAs) can post-transcriptionally regulate gene expression and are involved in the immune response. Excessive immune response to the gut microbiota plays a major role in the pathogenesis of Crohn's disease (CD). Regarding the role of miRNAs in immune response, this study aimed to investigate the contribution of miRNAs in the pathogenesis of CD. A total of 53 participants, including 23 CD patients and 30 healthy controls (HCs) were enrolled in this study. miRNAs, including miR-21, miR-29a, miR-29b, miR-31, miR-146a, miR-155, miR-181a, and miR-181c were evaluated via TaqMan MicroRNA Assays. Among the eight miRNAs, the amounts of miR-146a and miR-21 were significantly decreased in the CD patients relative to HC subjects. Moreover, we showed that there was a negative correlation between miR-146a and Harvey-Bradshaw index (HBI), as well as a positive correlation of miR-21 and miR-29b with HBI. Under-expression of miR-146a and miR-21, which are critical for the regulatory function of regulatory T cells (Tregs), is remarkably associated with CD.