Pehrsonhardin8546

Major depressive disorder (MDD) and suicide have been associated with elevated indices of oxidative damage in the brain, as well as white matter pathology including reduced myelination by oligodendrocytes. Oligodendrocytes highly populate white matter and are inherently susceptible to oxidative damage. Pathology of white matter oligodendrocytes has been reported to occur in brain regions that process behaviors that are disrupted in MDD and that may contribute to suicidal behavior. The present study was designed to determine whether oligodendrocyte pathology related to oxidative damage extends to brain areas outside of those that are traditionally considered to contribute to the psychopathology of MDD and suicide. Relative telomere lengths and the gene expression of five antioxidant-related genes, SOD1, SOD2, GPX1, CAT, and AGPS were measured in oligodendrocytes laser captured from two non-limbic brain areas occipital cortical white matter and the brainstem locus coeruleus. Postmortem brain tissues were obtained from brain donors that died by suicide and had an active MDD at the time of death, and from psychiatrically normal control donors. Relative telomere lengths were significantly reduced in oligodendrocytes of both brain regions in MDD donors as compared to control donors. Three antioxidant-related genes (SOD1, SOD2, GPX1) were significantly reduced and one was significantly elevated (AGPS) in oligodendrocytes from both brain regions in MDD as compared to control donors. These findings suggest that oligodendrocyte pathology in MDD and suicide is widespread in the brain and not restricted to brain areas commonly associated with depression psychopathology.Four unknown meroterpenoids named as psidials D-G (1-4) together with 5 known compounds (5-9) had been obtained from the leaves of Psidium guajava. Their absolute structures were elucidated by spectral and calculated methods. Psidials DF (1-3) represented unknown carbon skeleton of the 3,5-diformylbenzyl phloroglucinol-coupled sesquiterpenoid. The possible biosynthetic pathway for 1-3 was postulated. In the bioactivity assay, psidial F (3) was found to possess anti-inflammatory and anticoagulant activities.Eight new sesquiterpenoid dimers, artatrovirenolides A-H (1-8), along with three known analogues (9-11), were isolated from Artemisia atrovirens by using the LC-MS guided isolation. Compound 1 was a compound dimerized from a guaianolide and a 1,10-seco-guaianolide unit while others were from two guaianolide units. Angiogenesis inhibitor Their structures were established by comprehensive analysis of spectroscopic data, and their absolute configurations were determined by the aid of time-dependent density functional theory electronic circular dichroism (TDDFT ECD) calculation. Compound 8 showed anti-inflammatory effect in LPS-stimulated BV-2 microglial cells at 1 μM, while compounds 1, 2, 5, and 6 inhibited microglial inflammation at 10 μM.Methamphetamine (METH) use, and misuse are associated with severe socioeconomic consequences. METH users develop tolerance, lose control over drug taking behaviors, and suffer frequent relapses even during treatment. The clinical course of METH use disorder is influenced by multifactorial METH-induced effects on the central and peripheral nervous systems. Although these METH-induced consequences are observed in humans of all ages, races, and sexes, sexual dimorphism in these outcomes have been observed in both pre-clinical and clinical settings. In this review, we have provided a detailed presentation of the sex differences reported in human and animal studies. We have therefore presented data that identified the influences of sex on METH pharmacokinetics, METH-induced changes in behaviors, cognitive processes, structural changes in the brain, and the effects of the drug on neurotransmitter systems and molecular mechanisms. Finally, we highlighted the potential significance of sex as a critical variable that should be considered when planning the development of new pharmacotherapeutic approaches against MEH use disorder in humans.Cytosine-rich DNA can fold into four-stranded intercalated structures called i-motifs (iMs) under acidic conditions through the formation of hemi-protonated CC+ base pairs. However, the folding and stability of iMs rely on many other factors that are not yet fully understood. Here, we combined biochemical and biophysical approaches to determine the factors influencing iM stability under a wide range of experimental conditions. By using high-resolution primer extension assays, circular dichroism, and absorption spectroscopies, we demonstrate that the stabilities of three different biologically relevant iMs are not dependent on molecular crowding agents. Instead, some of the crowding agents affected overall DNA synthesis. We also tested a range of small molecules to determine their effect on iM stabilization at physiological temperature and demonstrated that the G-quadruplex-specific molecule CX-5461 is also a promising candidate for selective iM stabilization. This work provides important insights into the requirements needed for different assays to accurately study iM stabilization, which will serve as important tools for understanding the contribution of iMs in cell regulation and their potential as therapeutic targets.This study proposes a new model for the spatiotemporal prediction of PM2.5 concentration at hourly and daily time intervals. It has been constructed on a combination of three-dimensional convolutional neural network and gated recurrent unit (3D CNN-GRU). The performance of the proposed model is boosted by learning spatial patterns from similar air quality (AQ) stations while maintaining long-term temporal dependencies with simultaneous learning and prediction for all stations over different time intervals. 3D CNN-GRU model was applied to air pollution observations, especially PM2.5 level, collected from several AQ stations across the city of Tehran, the capital of Iran, from 2016 to 2019. It could achieve promising results compared to the methods such as LSTM, GRU, ANN, SVR, and ARIMA, which are recently introduced in the literature; it estimates 84% (R2 = 0.84) and 78% (R2 = 0.78) of PM2.5 concentration variations for the next hour and the following day, respectively.To better understand the origins and photochemical processing (aging) of organic aerosols (OA), we studied fine aerosols (PM2.5) collected at urban (Nankai District (ND)) and suburban (Haihe Education Park (HEP)) Tianjin, North China over a one-year period (2018-2019) for stable carbon isotopic composition (δ13C) of water-soluble diacids, oxoacids, α-dicarbonyls and fatty acids. Maleic (M, -18.3 ± 10.9‰ at ND and -23.5 ± 10.2‰ at HEP) and fumaric (F, -22.0 ± 12.1‰ at ND and -22.5 ± 10.5‰ at HEP) acids were found to be most enriched with 13C followed by oxalic acid (C2, -24.7 ± 3.9‰ at ND and -25.9 ± 4.7‰ at HEP) during the campaign. Based on seasonal changes in δ13C of selected marker species C6 and C9 diacids, phthalic, glyoxylic and pyruvic acids and glyoxal, and their comparison with the source signatures, we found that water-soluble OA in Tianjin were mainly originated from fossil fuel combustion and biomass burning emissions and were subjected for significant aging. The contribution from fossil fuel combustion including coal combustion was high in autumn and winter, especially at ND. Considering the enrichment of 13C in specific species together with linear relations of δ13C of selected species with their concentrations, with mass ratios and with the relative abundance of C2 diacid, we inferred that the photochemical transformations of longer-chain diacids, oxidation of α-dicarbonyls (Gly and mGly), preferably in gas phase, were important in warm period (March-September), whereas the oxidation of Gly, mGly and other precursors in aqueous phase were major in cold period (October-February).Dump fires are a significant environmental problem in post-mining areas. The TEXMIN project has shown that climate change could lead to more extreme weather events in the future, intense precipitation among them. The impact of water erosion on the development of endogenous fires in coal waste heaps has not been investigated thus far. Meteorological data collected from the studied dump area in Libiąż, Poland confirmed that heavy rainfall occurred many times, causing surface erosion on the slope. Gully erosion was observed on the western slope of the heap, the depth of which was up to 1.6 m. Data showed that between areas with and without water erosion, there was a significant difference in measured temperatures and gas concentrations that defined the fire intensity. Erosion facilitated self-heating such that internal temperatures increased to 52.9 °C. Further, at a depth of 1 m in the self-heating zone, maximum gas concentrations were 15.65 vol%. CO2, 10 ppm CO, and 0.435 vol%. CH4, while the O2 concentration dropped below 1.0 vol%. These results show that despite preventative measures, thermal activity reactivated in the vicinity of gully erosion and caused the self-heating zone to expand.Most glaciers worldwide are undergoing climate-forced recession, but the impact of glacier changes on biogeochemical cycles is unclear. This study examines the influence of proglacial sediment weathering on meltwater chemistry at the early stages of glacier recession in the High Arctic of Svalbard. Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) in combination with a wide range of geochemical analyses were used in this study. The SEM-EDS analyses of sediments collected in front of Werenskioldbreen show general degradation of pyrite and carbonate grains with age. The outer parts of pyrite grains have a gradual decrease in sulphur and gradual increase in iron oxides due to pyrite oxidation. This process was less advanced in the proglacial zone younger than 100 years compared to older sites such as the terminal moraine from the Little Ice Age. In both the proglacial zone and the terminal moraine, physical weathering of mineral grains, including formation of microcracks and microfractures, clearly enhanced pyrite oxidation. A consequence of proglacial sediment weathering is that the river chemistry is strongly affected by carbonate dissolution driven by sulphuric acid from sulphide oxidation. Also, reactive iron oxides, a product of sulphide oxidation, are mobilized in the proglacial zone. The results of this study show that proglacial weathering in the High Arctic of Svalbard is strongly coupled to river geochemistry, especially during the early stages of proglacial exposure after glacier recession.As human population growth has expanded in Southwest Florida, water quality has become degraded with an increased occurrence of harmful algal blooms (HABs). Red tide (Karenia brevis) originating offshore, intensifies in nearshore waters along Florida's Gulf Coast, and blue-green algae (Microcystis spp.) originating in Lake Okeechobee is discharged into the Caloosahatchee River. These HABs could be enhanced by anthropogenic nitrogen (N) and phosphorus (P) from adjacent watersheds. North Fort Myers is a heavily developed, low-lying city on the Caloosahatchee River Estuary serviced by septic systems with documented nutrient and bacterial pollution. To identify sources of pollution within North Fort Myers and determine connections with downstream HABs, this multiyear (2017-2020) study examined septic system- groundwater- surface water couplings through the analysis of water table depth, nutrients (N, P), fecal indicator bacteria (FIB), molecular markers (HF183, GFD, Gull2), chemical tracers (sucralose, pharmaceuticals, herbicides, pesticides), stable isotopes of groundwater (δ15N-NH4, δ15N-NO3) and particulate organic matter (POM; δ15N, δ13C), and POM elemental composition (CNP).