Pearsontoft3996

The mechanism of how plant-derived nanovesicles are uptaken by cells remains unknown. In this study, the garlic-derived nanovesicles (GDVs) were isolated and digested with trypsin to remove all surface proteins. Digested GDVs showed less uptake compared to undigested GDVs, confirming that the surface proteins played a role in the endocytosis. On the cell side (HepG2), interestingly, blocking the CD98 receptors significantly reduced the uptake of GDVs. During the cellular internalization of GDVs, we observed that some surface proteins of GDVs were co-localized with CD98. A total lysate of the GDV surface showed a high presence of a mannose-specific binding protein, II lectin. Blocking GDV II lectin (using mannose preincubation) highly reduced the GDV internalization, which supports that direct interaction between II lectin and CD98 plays an important role in internalization. The GDVs also exhibited in vitro anti-inflammatory effect by downregulating proinflammatory factors on the HepG2 cells. This work contributes to understanding a part of the GDV internalization process and the cellular anti-inflammatory effects of garlic.Fabricating perovskite single-crystal thin films (SCTFs) in controllable manner is the major challenge for the promising potential applications in optoelectronic devices. Although modifying the substrate surface is frequently used to realize the controlled growth of perovskite SCTFs, it is still unclear how the substrate condition affects the crystallization process. In this work, we systemically investigated the effects of the surface hydrophobicity of indium tin oxide substrates on the crystallization process of MAPbBr3 SCTFs prepared by the space-confined method. Comprehensive characterizations show that the surface morphology and crystallinity of SCTFs are improved, and the defect density is reduced when increasing the substrate hydrophobicity. The best MAPbBr3 thin film obtained has a full width at half-height of the rocking curve of the (001) crystal plane of 0.044°. The mechanism of the substrate hydrophobicity on the crystal growth is also discussed. These results will provide guidance to the controllable growth of high-quality SCTFs for perovskite SCTF devices.The contamination of water systems by heavy metals greatly threatens human health and ecological safety. An efficient adsorbent is critical for the removal of these contaminants. In this work, magnetic Ni3Si2O5(OH)4 nanotubes (NTs) have been synthesized via in situ hydrothermal reduction and further functionalized by grafting poly(4-vinyl pyridine) (P4VP) brushes on its surface via atom transfer radical polymerization. Characterizations by Fourier transform infrared, X-ray diffraction, thermogravimetric analysis, transmission electron microscopy, and X-ray photoelectron spectroscopy proved that P4VP was successfully grafted on the surface of magnetic Ni3Si2O5(OH)4 NTs. The resultant Ni3Si2O5(OH)4-g-P4VP NTs are efficient nanosorbents for removing Cr(VI) anions from water. The Cr(VI) adsorption capacity of Ni3Si2O5(OH)4-g-P4VP NTs reaches 1.49 mmol/g at a pH of 3. The pseudo-second-order kinetic model and the Freundlich isothermal model are suitable to describe the adsorption process. The analysis using Weber-Morris and Boyd models indicates that both intraparticle diffusion and external film diffusion affect the Cr(VI) adsorption process. The adsorption enthalpy is estimated to be 18.37 kJ/mol. More than 90% of the Cr(VI) adsorption capacity of the Ni3Si2O5(OH)4-g-P4VP NTs remains after eight adsorption and desorption cycles.m-Cresol is an important chemical material, which is mainly derived from low-temperature coal tar. In this work, for separating m-cresol from coal tar model oil, two propylamine-based ionic liquids (ILs) propylamine formate ([PA][FA]) and propylamine acetate ([PA][Ac]) were selected as extractants. The selected ILs were synthesized and characterized by Fourier transform infrared (FT-IR) and 1H nuclear magnetic resonance (NMR) spectroscopy. The effects of temperature, mass ratio of IL to model oil, and separation time on the separation efficiency of m-cresol were explored. The separation efficiency (SE) and distribution coefficient (D) were calculated from the experimental data to assess the separation performance of [PA][FA] and [PA][Ac]. The results showed that propylamine formate was a promising extractant with the separation efficiency of 97.8% and distribution coefficient of 27.59 at 298.15 K and mIL/moil = 0.2. In the meantime, molecular dynamics (MD) simulations were employed to comprehend the interaction mechanism, from which the noncovalent interaction energy (IE), radial distribution function (RDF), spatial distribution function (SDF), and averaged noncovalent interaction (aNCI) were calculated. The results showed that both cation and anion formed hydrogen bonds with m-cresol and the anions played a leading role with electrostatic interaction energy in separating m-cresol. In addition, the regeneration and reuse of the ionic liquids were explored.We have prepared yttrium (Y)-doped hydrogen titanate nanorods (HTN) by a microwave-assisted hydrothermal method. Y-doped HTN showed much improved photocatalytic activities for both H2 evolution and dye decomposition. H2 production from a methanol-water solution under UV-visible light for 7 h was enhanced by a factor of 5.5 with 1 wt % Y-doping. Doping with Y3+ ions reduced the band gap of HTN by ∼0.28 eV and induced new phases of anatase and rutile. High photocatalysis by Y-doping was attributed to enhanced light absorption (smaller band gap) and effective charge separation (heterojunction). To optimize H2 production, a series of experiments examining effects of doping concentrations and non-noble surface metal (e.g., Ni, Cu, Co) loading were carefully performed. see more Y-doping in this work is a new and promising approach for synthesizing highly active HTN by producing the HTN/rutile/anatase heterostructure within the one-pot method.This paper presents a peptide-mediated immunomagnetic separation technique and an immunofluorescence quantum dot technique for simultaneous and rapid detection of Escherichia coliO157H7, Staphylococcus aureus, and Vibrio parahaemolyticus. First, three peptides that can specifically recognize the three foodborne pathogens were combined with magnetic nanoparticles to form an immunomagnetic nanoparticle probe for capturing three kinds of target bacteria and then added three quantum dot probes (quantum dots-aptamer), which formed a sandwich composite structure. When the three quantum dot probes specifically combined with the three pathogenic bacteria, the remaining fluorescent signal in the supernatant will be reduced by magnetic separation. Therefore, the remaining fluorescent signal in the supernatant can be measured with a fluorescence spectrophotometer to indirectly determine the three pathogens in the sample. The linear range of the method was 10-107 cfu/mL, and in the buffer, the detection limits of E. coliO157H7, S.