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According to the report of the renewable energy policy network for the 21st century published in 2014, biodiesel and bioethanol are the most used biofuels and are responsible for transportation worldwide. Biodiesel specially has shown an increase in production globally by 15 times by volume from 2002 to 2012. Promising feedstock of biodiesel are cyanobacteria and microalgae as they possess a shorter cultivation time (4 fold lesser) and high oil content (10 fold higher) than corn, jatropha and soybean (conventional oil-producing territorial plants). Various valuable natural chemicals are also produced from these organisms including food supplements such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), pigments, and vitamins. Additionally, cellular components of microalgae and cyanobacteria are connected with therapeutic characteristics such as anti-inflammatory, antioxidant, antiviral and immune stimulating. Commercialization of algal biodiesel (or other products) can be achieved by isolating and identifying the high-yielding strains that possess a faster growth rate. Indigenous strains can be genetically engineered into high-yielding transgenic strains. The present article discusses about the use of nanotechnology and genetic engineering approach for improved lipid accumulation in microalgae for biodiesel production.Background Atrial septal aneurysm (ASA) is an uncommon cardiac anomaly that increases maternal morbidity during pregnancy. ASA is related to atrial arrhythmia thromboembolism and it may accompany congenital anomalies such as an atrial septal defect (ASD) or patent foramen ovale (PFO). There are no studies examining pregnancy outcomes in pregnant women with ASA. We aimed to investigate the cardiologic parameters and obstetric outcomes of pregnant women diagnosed with ASA. Iruplinalkib molecular weight Methods This prospective cohort study analyzed 45 pregnant women diagnosed with ASA, who continued their follow-ups in an obstetric tertiary care center. Results A total of 45 pregnant women were recruited; seven pregnancies ended before the 20th gestational week (six spontaneous abortion, one fetal anomaly), 38 women gave birth. In total, there were 32 term births (≥37 weeks), six preterm births ( less then 37 weeks), two extremely preterm births less then 28 weeks). Among 38 babies delivered, several obstetrical complications such as oligohydramnios, spontaneous preterm labor, intrauterine growth restriction (IUGR), preeclampsia and gestational diabetes mellitus (GDM) developed in 16 patients, while 22 women ended with term pregnancy without any complications. Deep venous thrombosis (DVT) developed in one patient. Conclusion ASA may have an increased risk for cardiac complications during pregnancy and may also be associated with poor pregnancy outcomes. Increased attention to these entities with more studies is needed in order to determine a potential risk for pregnant women.Objectives To determine the prevalence of serum zinc deficiency and provide the age- and sex-specific percentile values of serum zinc in children and adolescents. Methods We used the gathered data through the CASPIAN-V study, a national survey conducted on 3500 students aged 7-18 years from 30 provinces of Iran. In this study, 1370 blood samples were selected randomly, and serum zinc concentration was measured using a Hitachi automated analyzer. Zinc deficiency was defined as a serum zinc level of less than 75 μg/dL. Age-sex specific reference percentile values were developed for serum zinc concentration. Results The mean age of participants was 12.4 ± 3.0 years; 49.3% were girls and 73% were urban inhabitants. Mean (standard deviation [SD]) of serum zinc concentration was 107.23 (25.81) μg/dL with a significant sex difference; 109.03 ± 26.12 μg/dL for males compared to 105.41 ± 25.3 μg/dL for females (p = 0.009). The prevalence of subclinical zinc deficiency was 4.9% (95% confidence intervals [CI] 3.0, 6.9) in children and adolescents. Both zinc deficient and sufficient groups were similar in terms of age, sex and residential areas (all p-value > 0.05). Overall, the 5th and 95th percentile values for serum zinc were 68.28 and 151.87 μg/dL, respectively. The value of all percentiles consistently decreased with age. The 10-99th percentile values for serum zinc were greater in boys than girls at all ages. Conclusions Nearly 5% of subjects had zinc deficiency. Age-sex specific percentile values were established for Iranian children and adolescents.Background Sericin is a widely used protein in the pharmaceutical industry derived from the silkworm, Bombyx mori, and used for the treatment of various diseases and pathological conditions. It is the main ingredient of the Unani preparation khameera abresham. The study was conducted to evaluate the preclinical toxicity of the silk protein sericin in mice. Methods In the acute toxicity study, sericin was administered once orally to different groups of animals at doses of 500, 1000, and 2000 mg/kg. Animals were observed for 14 days. In the sub-acute toxicity study, sericin was administered in mice for 4 weeks in the toxic group at doses of 500, 1000, and 2000 mg/kg, while in the recovery group it was administered for 4 weeks at doses of 500 and 2000 mg/kg followed by 2 weeks of distilled water administration. Results In the acute toxicity study, the observed parameters showed no significant difference, and no mortality was reported. In the sub-acute toxicity study, there were no toxicological effects in any of the estimated parameters, while histopathological analysis showed inflammation in vital organs at the dose of 2000 mg/kg. Conclusions Results of our acute toxicity study suggest that sericin is safe at all administered doses, while the sub-acute study suggests that the NOAEL (no-observed-adverse-effect level) of sericin is below 2000 mg/kg, at which it can be considered safe.Background Natural anti-inflammatory nutraceuticals may be useful in suppressing the incessant aggravation of rheumatoid arthritis. Chia seeds as a natural source of antioxidants help prevent several oxidative stress-mediated diseases. The current study was focused on arthritis combined with obesity and evaluated the validation of oil and mucilage extracted from chia seeds as anti-inflammatory nutraceuticals in obese and non-obese adjuvant arthritic rat model. Methods Chia seeds oil was extracted by pressing method, whereas the mucilage was extracted using water (50 °C for 30 min). Oil and freeze-dried mucilage were tested for their anti-inflammatory effects using 48 male Sprague-Dawley rats. Obesity was developed in rats after 8 weeks of feeding on high-fat high-sucrose diet; on the first day of the ninth week, chia seeds oil and mucilage were administrated for 21 days, and arthritis was induced either in obese or non-obese rats via the injection with Freund's complete adjuvant. Swelling of the paw was then measured.

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