Norrishopper9924

CONCLUSION Despite mixed results, the findings of various preclinical studies are generally supportive of the involvement of DHEA and DHEAS in the pathophysiology of Alzheimer's disease, showing some promise for potential benefits of these neurosteroids in the prevention and treatment. However, so far small clinical trials brought little evidence to support their therapy in AD. Therefore, large-scale human studies are needed to elucidate the specific effects of DHEA and DHEAS and their mechanisms of action, prior to their applications in clinical practice. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.AIM This study was focused on the formulation of multi-unit extended-release peroral delivery device of lamotrigine for better management of epilepsy. BACKGROUND The single-unit extended-release peroral preparations often suffer from all-or-none effect. A significant number of multi-unit delivery systems have been reported as solution to this problem. But most of them are found to be composed with synthetic, semi-synthetic or their combination having physiological toxicity as well as negative environmental impact. Therefore, fabrication and formulation of multi-unit extended-release peroral preparations with natural nontoxic biodegradable polymers employing green manufacturing process are being appreciated worldwide. OBJECTIVE Lamotrigine-loaded extended release multi-unit beads have been fabricated with incorporation of a natural polysaccharide Cassia fistula seed gum in calcium-cross-linked alginate matrix employing a simple green process and 23 full factorial design. METHODS The total polymer concentrationined-release drug products by QbD route. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.Nanotechnology is currently the new hot topic in dermatology and nutraceutical/cosmeceutical delivery, owing to the advantages it provides in terms of enhancing the skin permeation of drugs, as well as increasing their therapeutic efficacy in the treatment of different dermatological diseases. There is also a great interest in the topical delivery of nutraceuticals; which are natural compounds with both therapeutic and cosmetic benefits, in order to overcome the side effects of topically applied chemical drugs. Quercetin is a key nutraceutical with topical antioxidant and anti-inflammatory properties which was reported to be effective in the treatment of different dermatological diseases, however, its topical therapeutic activity is hindered by its poor skin penetration. This review highlights the topical applications of quercetin, and summarizes the nanocarrier-based solutions to its percutaneous delivery challenges. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.AIMS Development of orodispersible tablets using different methodologies Background Orodispersible tablets (ODTs) are an option to facilitate the intake of pharmaceutical solid dosage forms, which dissolve in the mouth within 30 seconds releasing the drug immediately with no need for water intake or chewing. OBJECTIVE The main goal of our study is the technological development of lactose free orodispersible tablets that contain ketoprofen. METHOD We assessed different variables during the pharmacological development of ODTs compression techniques conducted after a wet granulation process, aiming to optimize the flow properties of the formulation, and a suspension freeze-drying molded in blisters. We developed three formulations for each method, each containing one of the superdisintegrants croscarmellose, crospovidone, or starch glycolate. RESULT During the production of ODTs, we performed a quality control of the granulation process, since the production of pellets contributed to the enhancement of the disintegration time and content homogeneity. Selleck Entospletinib Quality control tests for ODTs produced by freeze-drying were also satisfactory, despite significant changes in the final physical aspect of these products when compared to that of ODTs produced by compression. In addition, the disintegration times of ODTs produced by freeze-drying were substantially higher. Furthermore, these tablets displayed greater friability and pose a challenge to the control of a standard individual weight. CONCLUSION Among the superdisintegrants, croscarmellose contributed the most significantly to reduce the disintegration time and to dissolve the KTP effectively in 20 minutes. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Different approaches have been investigated to develop a preventive or therapeutic vaccine although none of them has been fully practical. Therapeutic vaccines against HIV-1 have been studied with the aim of elimination the virus from reservoir cells with or without HAART (highly active antiretroviral therapy). Fusion proteins with the most immunogenic features among conserved regions can facilitate this achievement in such a variable virus. To achieve the most immunogenic and also conserved regions, bioinformatic tools are widely used to predict antigens' features before applying them. OBJECTIVE This study aimed at in vitro evaluation of p24 -Nef fusion protein based on the previous in silico design to achieve a potential therapeutic subunit vaccine against HIV-1. METHODS The truncated form of p24-Nef using AAY flexible linker and the full protein were expressed and evaluated in prokaryotic system and confirmed by western blotting. We also used pcDNA3.1 to transfect Lenti-X 293T cells. Moreover, lentiviral vectors were applied to produce recombinant virions harboring the genes of interest and cell transduction. RESULTS Both fusion proteins in a truncated and a full form were expressed and confirmed by Anti Nef polyclonal antibody in western blotting. Recombinant virions were generated and transduced Lenti-X 293T cells confirming by immunefluorescence microscope and p24 ELISA assay kit. Transduced cells were analyzed by SDS-PAGE and western blotting which resulted in approved protein expression. CONCLUSION Fusion protein of p24 and Nef is well expressed in eukaryotic cell lines according to its pre-evaluated features by bioinformatic tools. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.