Nevillehatcher2172

Decreased myocardial contractility occurs with reduced maximum myofilament force of contraction and amplitude of transient intracellular Ca2+ concentration, both phenomena completely attenuated by tempol supplementation. #link# However, tempol only partially prevented shift of heart myosin heavy chain from dominant α-to β-isoform of ovariectomized rats. Immunoblot analysis of protein carbonylation indicated that tempol supplementation significantly reduced the level of cardiac myofibrillar proteins oxidation increased in ovariectomized rat heart. Taken together, the results indicate changes of cardiac contractile machinery following loss of female sex hormones were, in part, due to an increase in oxidative stress, and antioxidant supplementation could be considered another potential prevention measure in postmenopausal women.DNA copy number alterations (CNAs) are promising biomarkers to predict prostate cancer (PCa) outcome. However, fluorescence in situ hybridization (FISH) cannot assess complex CNA signatures because of low multiplexing capabilities. Multiplex ligation-dependent probe amplification (MLPA) can detect multiple CNAs in a single PCR assay, but PCa-specific probe mixes available commercially are lacking. Synthetic MLPA probes were designed to target 10 CNAs relevant to PCa 5q15-21.1 (CHD1), 6q15 (MAP3K7), 8p21.2 (NKX3-1), 8q24.21 (MYC), 10q23.31 (PTEN), 12p13.1 (CDKN1B), 13q14.2 (RB1), 16p13.3 (PDPK1), 16q23.1 (GABARAPL2), and 17p13.1 (TP53), with 9 control probes. In cell lines, CNAs were detected when the cancer genome was as low as 30%. Compared with FISH in radical prostatectomy formalin-fixed, paraffin-embedded samples (n = 18 15 cancers and 3 matched benign), the MLPA assay showed median sensitivity and specificity of 80% and 93%, respectively, across all CNAs assessed. In the validation set (n = 40 20 tumors sampled in two areas), the respective sensitivity and specificity of MLPA compared advantageously with FISH and TaqMan droplet digital PCR (ddPCR) when assessing PTEN deletion (FISH 85% and 100%; ddPCR 100% and 83%) and PDPK1 gain (FISH 100% and 92%; ddPCR 93% and 100%). This new PCa probe mix accurately identifies CNAs by MLPA across multiple genes using low quality and quantities (50 ng) of DNA extracted from clinical formalin-fixed, paraffin-embedded samples.The transcription factor JunB can induce physiological or pathological responses to various stimuli, including immune stimulants and bacteria, and plays an important role in the immune response process. In this study, we identified a JunB family member in Schizothorax prenanti (S. prenanti), which was designated SpJunB. The complete coding sequence (CDS) of SpJunB was 930 bp in length, which was submitted to GenBank (ID MN215886). SpJunB encodes a putative protein of 309 amino acids, which is highly homologous to JunB of common carp. The SpJunB protein contained a conserved JunB domain, and its 3D structure was also highly similar to (77.61%) the human SpJunB protein. SpJunB was found to be extensively expressed in various tissues, with the highest expression in the spleen. The expression of SpJunB was significantly upregulated after Aeromonas hydrophila (A. hydrophila) challenge. Prokaryotic expression indicated that a 51 kDa recombinant protein was obtained after induction with 1.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) for 6 h at 37 °C. The expression levels of IL-1β, IL-6 and IL-8 were significantly upregulated (p less then 0.01) after treatment of S. prenanti with the SpJunB protein. The activities of SOD, AKP and LZM were also significantly increased (p less then 0.01) after the treatment of S. prenanti with the SpJunB protein. Simultaneously, the SpJunB protein reduced the infection rate of A. hydrophila in S. prenanti. In conclusion, SpJunB may improve the immune functions of S. prenanti. It will be beneficial to further study the immune mechanism of JunB in fish.Combining ion-imprinting technology with pH-dependent adsorptive features of acid- or salt-activated zeolites brings up the opportunity to develop composite polymer materials with 'desired' sorption properties and performances. In this respect, we present here Co2+-imprinted composite cryo-beads with switching on/off selectivity towards the template ions, engineered by selecting the appropriate zeolite-treatment conditions and/or controlling the initial sorption pH values. Co2+ chelating efficiency of all cryo-beads was investigated either at pH 4 or 6 depending on zeolite conditioning strategy. The maximum sorption capacity values of ion-imprinted cryo-beads were from about 5 up to 7 times higher compared with those of non-imprinted ones. Under competitive conditions (Cu2+, Ni2+, Fe2+ and Cd2+ ions), the change of pH value from 4 to 6 resulted in a remarkable quenching of Co2+ selectivity generated by the zeolite shift from the H+-form to the Na+-form. The presence of zeolites within cryogel matrix generated composites with outstanding elasticity that allows the instant recovery of gels after full compression. These results indicate that the cryogel-type composites can be successfully re-used in separation processes for several times without losing their features.Type 2 diabetes (T2D) is a complicated endocrine metabolic disease, accompanied with oxidative stress injury and low-grade inflammation. The effects of polysaccharide extracted from Dendrobium officinale stem (DOP) on oxidative stress, inflammation and dysregulated metabolism in the liver of type 2 diabetic rats and its potential mechanism were evaluated in the study. Here, check details - quadrupole - time - of - flight (UPLC-Q-TOF) mass spectrometry-based lipidomics and metabolomics analysis were carried out to study the amelioration of DOP on the liver metabolism disorders of type 2 diabetic rats. Lipidomics analysis indicated that the disturbed degree of fatty acid, glycerolipid (diacylglycerol and triacylglycerol), and glycerophospholipid (phosphatidylcholine and phosphatidylethanolamine) metabolism were mitigated by the DOP treatment. Metabolomics analysis revealed that the DOP treatment balanced the metabolism of ceramide and bile acids, including deoxycholic acid, taurocholic acid, and cholic acid.