Murphywilder3477

Consensus design (CD) is a representative sequence-based protein design method that enables the design of highly functional proteins by analyzing vast amounts of protein sequence data. This study proposes a partial consensus design (PCD) of a protein as a derivative approach of CD. The method replaces the target protein sequence with a consensus sequence in a secondary-structure-dependent manner (i.e., regionally dependent and divided into α-helix, β-sheet, and loop regions). click here In this study, we generated several artificial partial consensus l-threonine 3-dehydrogenases (PcTDHs) by PCD using the TDH from Cupriavidus necator (CnTDH) as a target protein. Structural and functional analysis of PcTDHs suggested that thermostability would be independently improved when consensus mutations are introduced into the loop region of TDHs. On the other hand, enzyme kinetic parameters (kcat/Km) and average productivity would be synergistically enhanced by changing the combination of the mutations-replacement of one region of CnTDH with a consensus sequence provided only negative effects, but the negative effects were nullified when the two regions were replaced simultaneously. Taken together, we propose the hypothesis that there are protein regions that encode individual protein properties, such as thermostability and activity, and that the introduction of consensus mutations into these regions could additively or synergistically modify their functions.Cerebral malaria (CM) is caused by Plasmodium falciparum, resulting in severe sequelae; one of its pathogenic factors is the low bioavailability of nitric oxide (NO). Our previous study suggested that the combination of artesunate (AS) and tetramethylpyrazine (TMP) exerts an adjuvant therapeutic effect on the symptoms of experimental CM (ECM) and that NO regulation plays an important role. In the present study, we further verified the effects of AS+TMP on cerebral blood flow (CBF) and detected NO-related indicators. We focused on the role of NO through S-nitrosoproteome based on previous proteomics data and explored the mechanism of AS+TMP for improving pathological ECM symptoms. We observed that AS+TMP reduces adhesion, increases CBF, and regulates NO synthase (NOS) activity, thereby regulating the level of S-nitrosothiols, such as metabolism-related or neuro-associated receptors, for improving ECM symptoms. These results demonstrated that AS+TMP could be an effective strategy in adjuvant therapy of CM.Nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are natural biopolymers composed of nucleotides that store, transmit, and express genetic information. Overexpressed or underexpressed as well as mutated nucleic acids have been implicated in many diseases. Therefore, nucleic acid tests (NATs) are extremely important. Inspired by intracellular DNA replication and RNA transcription, in vitro NATs have been extensively developed to improve the detection specificity, sensitivity, and simplicity. The principles of NATs can be in general classified into three categories nucleic acid hybridization, thermal-cycle or isothermal amplification, and signal amplification. Driven by pressing needs in clinical diagnosis and prevention of infectious diseases, NATs have evolved to be a rapidly advancing field. During the past ten years, an explosive increase of research interest in both basic research and clinical translation has been witnessed. In this review, we aim to provide comprehensive coverage of the progress to analyze nucleic acids, use nucleic acids as recognition probes, construct detection devices based on nucleic acids, and utilize nucleic acids in clinical diagnosis and other important fields. We also discuss the new frontiers in the field and the challenges to be addressed.This paper demonstrates the feasibility of a long-range antenna sensor embedded underneath a liquid repellent fabric to be employed as a wearable sensor in personal protective fabrics. The sensor detects and monitors hazardous aqueous liquids on the outer layer of fabrics, to add an additional layer of safety for professionals working in hazardous environments. A modified patch antenna was designed to include a meandering-shaped resonant structure, which was embedded underneath the fabric. Superhydrophobic fabrics were prepared using silica nanoparticles and a low-surface-energy fluorosilane. 4 to 20 μL droplets representing hazardous aqueous solutions were drop-cast on the fabrics to investigate the performance of the embedded antenna sensor. Long-range (S21) measurements at a distance of 2-3 m were performed using the antenna sensor with treated and untreated fabrics. The antenna sensor successfully detected the liquid for both types of fabrics. The resonant frequency sensitivity of the antenna sensor underneath the treated fabric exhibiting superhydrophobicity was measured as 370 kHz/μL, and 1 MHz/μL for the untreated fabric. The results demonstrate that the antenna sensor is a good candidate for wearable hazardous aqueous droplet detection on fabrics.The syntheses and molecular structures of new SmII and TmII N,N-dimethylaminodiboranate (DMADB) complexes are described. Treating SmI2(THF)2 with Na(H3BNMe2BH3) in THF results in the formation of Sm(H3BNMe2BH3)2(THF)3 (1), which can be readily converted to Sm(H3BNMe2BH3)2(DME)2 (DME = 1,2-dimethoxyethane) or Sm(H3BNMe2BH3)2(diglyme) by exchange with the corresponding ether. We also show that Sm(H3BNMe2BH3)2(THF)3 can be prepared by reduction of the SmIII compound Sm(H3BNMe2BH3)3(THF) with KC8 and that addition of 18-crown-6 to this reaction mixture results in the formation of the SmII compound Sm(H3BNMe2BH3)2(18-crown-6). In a similar fashion, two new TmII complexes have been synthesized treatment of TmI2 in THF with Na(H3BNMe2BH3) results in the formation of Tm(H3BNMe2BH3)2(THF)2 and Tm(H3BNMe2BH3)2(THF)3, which form a cocrystal. IR data and elemental analyses are reported for all the new compounds, as are their crystal structures. 1H and 11B NMR data are provided where available.In this study, ginger residue from juice production was evaluated as a raw material resource for preparation of nanofiber hydrogels with multifunctional properties for advanced wound dressing applications. Alkali treatment was applied to adjust the chemical composition of ginger fibers followed by TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated oxidation prior to nanofiber isolation. The effect of alkali treatment on hydrogel properties assembled through vacuum filtration without addition of any chemical cross-linker was evaluated. An outstanding absorption ability of 6200% combined with excellent mechanical properties, tensile strength of 2.1 ± 0.2 MPa, elastic modulus of 15.3 ± 0.3 MPa, and elongation at break of 25.1%, was achieved without alkali treatment. Furthermore, the absorption capacity was tunable by applying alkali treatment at different concentrations and by adjusting the hydrogel grammage. Cytocompatibility evaluation of the hydrogels showed no significant effect on human fibroblast proliferation in vitro. Ginger essential oil was used to functionalize the hydrogels by providing antimicrobial activity, furthering their potential as a multifunctional wound dressing.Settlement and metamorphosis of planktonic larvae into benthic adults are critical components of a diverse range of marine invertebrate-mediated processes such as the formation of mussel beds and coral reefs, the recruitment of marine shellfisheries, and the initiation of macrobiofouling. Although larval settlement and metamorphosis induced by natural chemical cues is widespread among marine invertebrates, the mechanisms of action remain poorly understood. Here, we identified that the molecular target of adenosine (an inducer of larval settlement and metamorphosis from conspecific adults in the invasive biofouling mussel Mytilopsis sallei) is adenosine kinase (ADK). The results of transcriptomic analyses, pharmacological assays, temporal and spatial gene expression analyses, and siRNA interference, suggest that ATP-dependent phosphorylation of adenosine catalyzed by ADK activates the downstream AMPK-FoxO signaling pathway, inducing larval settlement and metamorphosis in M. sallei. This study not only reveals the role of the ADK-AMPK-FoxO pathway in larval settlement and metamorphosis of marine invertebrates but it also deepens our understanding of the functions and evolution of adenosine signaling, a process that is widespread in biology and important in medicine.We investigate the anti-Stokes Raman scattering of single carbyne chains confined inside double-walled carbon nanotubes. Individual chains are identified using tip-enhanced Raman scattering (TERS) and heated by resonant excitation with varying laser powers. We study the temperature dependence of carbyne's Raman spectrum and quantify the laser-induced heating based on the anti-Stokes/Stokes ratio. Due to its molecular size and its large Raman cross section, carbyne holds great promise for local temperature monitoring, with potential applications ranging from nanoelectronics to biology.Exploring nanostructured transition-metal sulfide anode materials with excellent electrical conductivity is the key point for high-performance alkali metal ion storage devices. Herein, we propose a powerful bottom-up strategy for the construction of a series of sandwich-structured materials by a rapid interfacial self-assembly approach. Oleylamine could act as a functional reagent to guarantee that the nanomaterials self-assemble with MXene. Benefiting from the small size of Co-NiS nanorods, excellent conductivity of MXene, and sandwiched structure of the composite, the Co-NiS/MXene composite could deliver a high discharge capacity of 911 mAh g-1 at 0.1 A g-1 for lithium-ion storage. After 200 cycles at 0.1 A g-1, a high specific capacity of 1120 mAh g-1 could be still remaining, indicating excellent cycling stability. For sodium-ion storage, the composite exhibits high specific capacity of 541 mAh g-1 at 0.1 A g-1 and excellent rate capability (263 mAh g-1 at 5 A g-1). This work offers a straightforward strategy to design and construct MXene-based anode nanomaterials with sandwiched structure for high-performance alkali metal ion storage and even in other fields.Achieving thermoelectric devices with high performance based on low-cost and nontoxic materials is extremely challenging. Moreover, as we move toward an Internet-of-Things society, a miniaturized local power source such as a thermoelectric generator (TEG) is desired to power increasing numbers of wireless sensors. Therefore, in this work, an all-oxide p-n junction TEG composed of low-cost, abundant, and nontoxic materials, such as n-type ZnO and p-type SnOx thin films, deposited on borosilicate glass substrate is proposed. A type II heterojunction between SnOx and ZnO films was predicted by density functional theory (DFT) calculations and confirmed experimentally by X-ray photoelectron spectroscopy (XPS). Moreover, scanning transmission electron microscopy (STEM) combined with energy-dispersive X-ray spectroscopy (EDS) show a sharp interface between the SnOx and ZnO layers, confirming the high quality of the p-n junction even after annealing at 523 K. ZnO and SnOx thin films exhibit Seebeck coefficients (α) of ∼121 and ∼258 μV/K, respectively, at 298 K, resulting in power factors (PF) of 180 μW/m K2 (for ZnO) and 37 μW/m K2 (for SnOx).