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her, the results of our study indicate that ambrosin sesquiterpene may be developed as a promising anticancer agent in human breast cancer provided further in-depth studies are performed.

Taken all together, the results of our study indicate that ambrosin sesquiterpene may be developed as a promising anticancer agent in human breast cancer provided further in-depth studies are performed.

The microRNAs (miRs) control a vast number of biological and cellular processes. miR-1 has been implicated in the development and progression in different types of cancers. Nonetheless, the function and therapeutic implications of miR-1 have not been studied in breast cancer. This study was undertaken to study the role of miR-1 in human breast cancer cells.

MBA-MD-231 breast cancer line and the normal MB-157 cell line were mainly used in this research. Expression analysis was performed by qRT-PCR. Dorsomorphin clinical trial Cell viability was determined by MTT assay and apoptosis was detected by acridine orange (AO)/ethidium bromide (EB) and DAPI staining. Transwell assay was used for cell migration and invasion and western blot analysis was used to determine the protein expression.

miR-1 was significantly but aberrantly suppressed in breast cancer cells relative to the MB-157 normal cells. Overexpression of miR-1 in MBA-MD-231 suppressed their proliferation dose-dependently. The inhibition of MBA-MD-231 cell proliferation was found to be due to induction of apoptosis. The apoptotic cell percentage was 37.1% in miR-1 mimics transfected in comparison to 3.7% in miR-negative control (NC) transfected cells. Additionally, miR-1 also suppressed the chemosensitivity of the MBA-MD-1 breast cancer cells to cisplatin. Transwell assay showed that miR-1 overexpression suppressed the migration and invasion of the MBA-MD-231 cells. The results clearly showed that overexpression of miR-1 suppressed the phosphorylation of MEK and ERK.

miR-1 acts as a tumor suppressor and may exhibit therapeutic implications in the treatment of breast cancer.

miR-1 acts as a tumor suppressor and may exhibit therapeutic implications in the treatment of breast cancer.

Long non-coding RNA (lncRNA) plasmacytoma variant translocation 1-214 transcript (PVT1-214) is a notable lncRNA involved in gastric cancer and colorectal cancer (CRC) so far. Nowadays, the biological function of PVT1-214 on the response of CRC to chemotherapy is still unclear. We aimed to explore the molecular mechanism of PVT1-214 and its regulatory mechanism in advanced CRC.

The levels of PVT1-214, microRNA (miR)-128, and interferon regulatory factor-1 (IRF-1) in CRC tissues and cell lines were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Log-rank test was applied to evaluate the role of high PVT1-214 levels in shortening the overall survival of CRC patients. Chi-square test was to assess the relation between PVT1-214 expression and clinicopathological features of CRC patients. CCK8 assays tested the cell proliferation of oxaliplatin-resistant CRC cells (HCT116/Oxa and SW480/Oxa) with PVT1-214 knockdown. The underlying regulatory mechanism between PVT1-214 and miR-128 was prel target for intervention in CRC.

IRF-1/PVT1-214 may markedly boost the oxaliplatin-resistance of CRC, resulting in the late TNM stage and poor survival. These findings suggest that the IRF-1/PVT1-214 axis may be a helpful target for intervention in CRC.

The purpose of our study was to investigate preoperative and intraoperative risk factors for anastomotic leak (AL) after elective colorectal resections performed for malignancies. In addition, we studied some features of postoperative recovery and their influence on AL occurrence.

We retrospectively reviewed the records of patients that underwent colorectal surgical procedures for malignancies between January 2013 and December 20017 in a single institution. Only procedures with primary anastomosis were included.

Of the 153 patients, 56.2% were male. The mean age was 67.5 years. AL occurred in 15 patients (9.8%). In univariate analysis, multiorgan resection, delayed postoperative bowel movement and delayed onset of per oral intake were significantly correlated with AL. Gender, preoperative albumin level, primary cancer site and surgery duration did not have significant correlation with AL.

Risk factors described in the literature of the 20th century are no longer current. The main findings that feature postoperative recovery were associated with increased risk of AL and should be more carefully investigated in further studies which could lead towards the development of new specific post-operative protocols.

Risk factors described in the literature of the 20th century are no longer current. The main findings that feature postoperative recovery were associated with increased risk of AL and should be more carefully investigated in further studies which could lead towards the development of new specific post-operative protocols.

The present study was conducted to evaluate the efficacy and safety of simultaneous resection of colorectal cancer (CRC) and synchronous liver metastases (SCRLM) in a group of elderly Chinese patients regarding the population aging in China.

From January 1st 2010 to May 1st 2015, 24 out of 32 elderly patients who underwent simultaneous resection of CRC and SCRLM were matched with 24 out of 55 young patients based on the propensity scores. Perioperative results and survival outcomes were compared.

The demographic and cancer characteristics were comparable between the two groups. The postoperative duration of intensive nursing care in the elderly group was significantly longer than that in the young group [5.00 (4.00-6.75) vs. 6.50 (5.00-9.00) days, p=0.038]. No significant between-group difference was observed with respect to time to first defecation, length of postoperative hospital stay, or postoperative complication rate. There was no significant difference with respect to 3-year overall and disease-free survival rates between the two groups.

Simultaneous resection of CRC and SCRLM was safe and feasible in elderly patients, with reasonable 3-year survival rates. Age per se should not be considered as a contraindication for simultaneous resection of CRC and SCRLM.

Simultaneous resection of CRC and SCRLM was safe and feasible in elderly patients, with reasonable 3-year survival rates. Age per se should not be considered as a contraindication for simultaneous resection of CRC and SCRLM.