Michaelsenmcdonald2433

All isolates tested were capable of producing symptoms on cotton, however isolate aggressiveness varied within and across inoculation assays. In all assays, higher levels of disease development were observed in the moderately susceptible Pima (G. barbadense L.) cultivars (DP-340 or PYH-830) when compared to the moderately tolerant Upland (G. hirsutum L.) cultivar (FM-2334). However, no correlation was found among the different response variables for the rolled towel assay when compared with the root dip and infested-oat seed assays. These results suggest that different genes are involved in the resistance response during the early seedling development stage measured in the rolled towel assay compared to the later seedling development stages measured during the root dip inoculation and infested-oat seed assays, revealing the complexity of the Fusarium wilt disease and host-plant resistance mechanisms.Leucophyllum frutescens (Scrophulariaceae family), commonly known as Texas sage or cenizo, is an evergreen shrub native to southwestern United States and northern Mexico. This plant is commercially sold as a native, drought-tolerant ornamental. During the spring of 2019 and 2020, typical symptoms of powdery mildew were found on cenizo plants growing as ornamentals in urban areas in the municipality of Ahome, Sinaloa, Mexico. Disease incidence was 95% from a sampled population of 120 plants. Initial symptoms of powdery mildew developed as irregular white colonies on upper leaf surfaces which expanded as infections progressed. In severe infections, leaves became distorted, exhibiting premature defoliation. Microscopic examination showed nipple-shaped appressoria. Conidiophores (n= 30) were hyaline, cylindrical, erect, 89.4 to 134.2 μm long, and forming catenescent conidia. Foot-cells were cylindrical, 35.7 to 65.3 × 10.2 to 13.5 μm, followed by 1-3 shorter cells. Conidia (n= 100) were hyaline, ellipsoid to ovoidery mildew on a member of Scrophulariaceae, specifically L. frutescens in Mexico and worldwide. Further studies for monitoring and control strategies of powdery mildew on Texas sage are required.Kiwifruit (Actinidia spp.) is an economically important fruit crop globally. China is the largest kiwifruit-growing country in the world, and Shaanxi Province is the major kiwifruit-growing region in China. A systematic survey detected various symptoms in kiwifruit plants grown in a commercial kiwifruit field in Shaanxi Province. Samples were collected from kiwifruit plants showing symptoms and used for virus detection by high-throughput sequencing. In addition to 10 known kiwifruit viruses, three new viruses were detected and tentatively named Actinidia yellowing ringspot virus (AYRSpV), Actinidia yellowing virus 1 (AcYV1), and Actinidia yellowing virus 2 (AcYV2). The genome sequences of the three new viruses and four known viruses were determined. Based on the demarcation criteria of the International Committee on Taxonomy of Viruses, AYRSpV might be a new member of the genus Ilarvirus in the family Bromoviridae, AcYV1 might be a new virus of the genus Waikavirus in the family Secoviridae, and AcYV2 might be a novel virus in the family Tombusviridae. Spherical viral particles were found in the samples infected with AYRSpV, AcYV1, and AcYV2 by transmission electron microscopy. Further analysis showed that all 13 viruses can infect both Actinidia deliciosa and A. chinensis but the incidences of these infections vary among different kiwifruit cultivars in different regions. These results provide valuable information for understanding the virome of kiwifruit in China.Amygdalus triloba (Rosaceae; previously Prunus triloba) is a deciduous, flowering shrub that is widely used in the greening and beautification of lawns, parks and courtyards in China. In late May 2019, a leaf spot disease of A. triloba was observed on approximately 35% of plants in the Xinjiang Alaer city (40˚33'20N, 81˚17'19E). The disease symptoms began as small, suborbicular, brown spots on the leaves. As the disease progressed, the spots enlarged and coalesced into large necrotic areas and resulted in premature defoliation. Leaf sections (5 x 5 mm) from infected leaves were surface - sterilized with 75% ethanol for 30 s and 0.1% HgCl2 for 1 min, rinsed three times in sterile distilled water and then incubated on potato dextrose agar (PDA). Fifteen fungal isolates showing similar morphological characteristics were obtained by single-spore isolation. On the PDA plates, all fungal colonies had a dark olive color with loose, cottony mycelium. On the potato carrot agar, the fungus formed unbranched spore cdson, G. C. 1995. Appl. Environ. Microbiol. 611323. Simmons, E. G. 2007. Alternaria An Identification Manual. CBS Fungal Biodiversity Centre, Utrecht, The Netherlands. White, T. J., et al. 1990. PCR Protocols A Guide to Methods and Applications. Academic Press, San Diego, CA. Xie, Y., et al. 2017. Liaoning Agricultural Sciences. 6 73.Rubbery taproot disease (RTD) of sugar beet was observed in Serbia for the first time in the 1960s. The disease was already described in neighboring Bulgaria and Romania at the time but it was associated with abiotic factors. In this study on RTD of sugar beet in its main growing area of Serbia, we provide evidence of the association between 'Candidatus Phytoplasma solani' (stolbur phytoplasma) infection and the occurrence of typical RTD symptomatology. 'Ca. P. solani' was identified by PCR and the sequence analyses of 16S ribosomal RNA, tuf, secY, and stamp genes. In contrast, the causative agent of the syndrome "basses richesses" of sugar beet-namely, 'Ca. Arsenophonus phytopathogenicus'-was not detected. Sequence analysis of the stolbur strain's tuf gene confirmed a previously reported and a new, distinct tuf stolbur genotype (named 'tuf d') that is prevalent in sugar beet. The sequence signatures of the tuf gene as well as the one of stamp both correlate with the epidemiological cycle and reservoir plant host. This study provides knowledge that, for the first time, enables the differentiation of stolbur strains associated with RTD of sugar beet from closely related strains, thereby providing necessary information for further epidemiological work seeking to identify insect vectors and reservoir plant hosts. The results of this study indicate that there are differences in hybrid susceptibility. Selleckchem PF-8380 Clarifying the etiology of RTD as a long-known and economically important disease is certainly the first step toward disease management in Serbia and neighboring countries.