Mcmillanjunker5882

These results corroborate existing evidence about a warmer and drier climate and the reported increase in the frequency and severity of drought as well as floods in the area, and add finer details to the changes in ecological, hydrological and human systems, which are not sufficiently reported in existing climate impact studies. We also flagged new observations in biological systems as pointers for further intensive investigation. Given the complexity associated with understanding impacts of climate change and the urgent need to refine knowledge about the same, we argued for perforation of the boundaries of climate science to accommodate enriching perceptions of Indigenous communities who have been religiously observing changes happening in their local environment, albeit being relegated.Triple-negative breast cancer (TNBC) is an aggressive, metastatic/invasive sub-class of breast cancer (BCa). Cell surface protein-derived multi-epitope vaccine-mediated targeting of TNBC cells could be a better strategy against the disease. Literature-based identified potential cell surface markers for TNBC cells were subjected to expression pattern and survival analysis in BCa patient sample using TCGA database. The cytotoxic and helper T-lymphocytes antigenic epitopes in the test proteins were identified, selected and fused together with the appropriate linkers and an adjuvant, to construct the multi-epitope vaccine (MEV). The immune profile, physiochemical property (PP) and world population coverage of the MEV was studied. Immune simulation, cloning in a suitable vector, molecular docking (against Toll-like receptors, MHC (I/II) molecules), and molecular dynamics simulations of the MEV was performed. Cell surface markers were differentially expressed in TNBC samples and showed poor survival in TNBC patients. Satisfactory PP and WPC (up to 89 and 99%) was observed. MEV significant stable binding with the immune molecules and induced the immune cells in silico. The designed vaccine has capability to elicit immune response which could be utilized to target TNBC alone/combination with other therapy. The experimental studies are required to check the efficacy of the vaccine.

The online version contains supplementary material available at 10.1007/s13205-022-03140-3.

The online version contains supplementary material available at 10.1007/s13205-022-03140-3.The increasing prevalence of ischemic stroke combined with limited therapeutic options highlights the compelling need for continued research into the development of future neuro-therapeutics. Death-Associated Protein Kinase 1 (DAPK1) and p53 protein-protein interaction serve as a signaling point for the convergence of apoptosis and necrosis in cerebral ischemia. In this study, we used an integrated chemo-informatics and in vitro experimental drug repurposing strategy to screen potential small-molecule inhibitors of DAPK1-p53 interaction from the United States of America Food and Drug Administration (FDA) approved drug database exhibiting post-ischemic neuroprotective and neuro-regenerative efficacy and mechanisms. The computational docking and molecular dynamics simulation of FDA-approved drugs followed by an in vitro experimental validation identified acarbose, an anti-diabetic medication and caloric restriction mimetic as a potential inhibitor of DAPK1-p53 interaction. The evaluation of post-ischemic neuropte the safety and neuroprotective efficacy of acarbose against ischemic stroke.

The online version contains supplementary material available at 10.1007/s13205-022-03130-5.

The online version contains supplementary material available at 10.1007/s13205-022-03130-5.Breast cancer is a heterogeneous disease with different intrinsic subtypes. find more The conventional treatment of surgical resection, chemotherapy, immunotherapy and radiotherapy has not shown significant improvement in the survival rate of breast cancer patients. The therapeutics used cause bystander toxicities deteriorating healthy tissues. The breakthroughs of nanotechnology have been a promising feat in selective targeting of tumor site thus increasing the therapeutic gain. By the application of nanoenabled carriers, nanomedicines ensure targeted delivery, stability, enhanced cellular uptake, biocompatibility and higher apoptotic efficacy. The present review focuses on breakthrough of nanoscale intervention in targeted drug delivery as novel class of therapeutics. Nanoenabled carriers like polymeric and metallic nanoparticles, dendrimers, quantum dots, liposomes, solid lipid nanoparticles, carbon nanotubes, drug-antibody conjugates and exosomes revolutionized the targeted therapeutic delivery approach. These nanoassemblies have shown additional effect of improving the solubility of drugs such as paclitaxel, reducing the dose and toxicity. The present review provides an insight on the different drug conjugates employed/investigated to curb breast cancer using nanocarrier mediated targeted drug delivery. However, identification of appropriate biomarkers to target, clearer insight of the biological processes, batch uniformity, reproducibility, nanomaterial toxicity and stabilities are the hurdles faced by nanodrugs. The potential of nano-therapeutics delivery necessitates the agglomerated efforts of research community to bridge the route of nanodrugs for scale-up, commercialization and clinical applications.MicroRNAs (miRNAs) play key regulatory roles in the plant's response to biotic and abiotic stresses and have fundamental functions in plant-virus interactions. The study of changes in miRNAs in response to virus infection can provide molecular details for a better understanding of virus-host interactions. Maize Iranian mosaic virus (MIMV) infects maize and certain other poaceous plants but miRNA changes in response to MIMV infection are unknown. In the present study, we compared the miRNA profiles of MIMV-infected and uninfected maize and characterized their predicted roles in response to the virus. Small RNA sequencing of maize identified 257 conserved miRNAs of 26 conserved families in uninfected and MIMV-infected maize libraries. Among them, miR395, miR166 and miR156 family members were highly represented. Small RNA data were confirmed using RT-qPCR. In addition, 33 potential novel miRNAs were predicted. The data show that 13 miRNAs were up-regulated and 113 were down-regulated in response to MIMV infection. Several of those miRNAs are known to be important in the response to plant pathogens. To determine the potential roles of individual miRNAs in response to MIMV, miRNA targets, predicted interactions with circular RNAs and comparative transcriptome data were analyzed. The expression profiles of different miRNAs in response to MIMV provide novel insights into the roles of miRNAs in the interaction between MIMV and maize plants.

The online version contains supplementary material available at 10.1007/s13205-022-03134-1.

The online version contains supplementary material available at 10.1007/s13205-022-03134-1.Production of biosurfactant by a novel indigenous isolate Pseudomonas otitidis strain DU13 and its role in bioremediation of petroleum hydrocarbon is reported. The identity of the isolate was confirmed by 16S rDNA gene sequencing analysis (Genbank accession MK177190). The biosurfactant produced by the isolate could reduce the surface tension of petroleum supplemented medium by 46% just after 7 days of treatment. The emulsification index (E 24 ) of the surfactant was found 37, 35, and 20%, respectively, against used motor oil, diesel, and kerosene. The FTIR spectrum of the crude biosurfactant showed the presence of υC-H stretch, υCH2, υ-C=C stretch and υC-H bonding. The isolated strain could degrade 26% of TPH content of used motor oil in liquid culture. Whereas, ex situ pilot-scale field trial demonstrated very high bioremediation potential of the isolate in terms of germination rate of Vigna radiata and Cicer arietinum seeds and plant growth just after 20 days of treatment.Pectin is one of the most important components of the plant cell wall. Galacturonosyltransferase-like (GATL) is an important enzyme involved in forming pectin in Arabidopsis thaliana. In this study, 12 PtGATL genes were identified and characterized based on the Populus trichocarpa genome using bioinformatics methods. The results showed that the PtGATLs contained four typical motifs, including DXD, LPPF, GLG, and HXXGXXKPW. According to phylogenetic analysis, PtGATLs were divided into six groups. Chromosome distribution and genome synteny analysis showed that there were 11 segmental-duplicated gene pairs with repeated fragments on chromosomes 2, 5, 7, 8, 10, and 14. Tissue-specific expression profiles indicated that these PtGATLs had different expression patterns. The transcription level of PtGATLs was regulated by different carbon dioxide and nitrogen concentrations. In conclusion, the identification and analysis of PtGATL genes in poplar provide important information on the gene function.

The online version contains supplementary material available at 10.1007/s13205-022-03129-y.

The online version contains supplementary material available at 10.1007/s13205-022-03129-y.This study is to introduce a novel design and implementation of a neuro-swarming computational numerical procedure for numerical treatment of the fractional Bagley-Torvik mathematical model (FBTMM). The optimization procedures based on the global search with particle swarm optimization (PSO) and local search via active-set approach (ASA), while Mayer wavelet kernel-based activation function used in neural network (MWNNs) modeling, i.e., MWNN-PSOASA, to solve the FBTMM. The efficiency of the proposed stochastic solver MWNN-GAASA is utilized to solve three different variants based on the fractional order of the FBTMM. For the meticulousness of the stochastic solver MWNN-PSOASA, the obtained and exact solutions are compared for each variant of the FBTMM with reasonable accuracy. For the reliability of the stochastic solver MWNN-PSOASA, the statistical investigations are provided based on the stability, robustness, accuracy and convergence metrics.Significant advancement in the field of nanotechnology has raised the possibility of applying potent engineered biocompatible nanomaterials within biological systems for theranostic purposes. Titanium dioxide (titanium(IV) oxide/titania/TiO2) has garnered considerable attention as one of the most extensively studied metal oxides in clinical applications. Owing to the unique properties of titania, such as photocatalytic activity, excellent biocompatibility, corrosion resistance, and low toxicity, titania nanomaterials have revolutionized therapeutic approaches. Additionally, titania provides an exceptional choice for developing innovative medical devices and the integration of functional moieties that can modulate the biological responses. Thus, the current review aims to present a comprehensive and up-to-date overview of TiO2-based nanotherapeutics and the corresponding future challenges.Piezoelectric materials have attracted more attention than other materials in the field of textiles. Piezoelectric materials offer advantages as transducers, sensors, and energy-harvesting devices. Commonly, ceramics and quartz are used in such applications. However, polymeric piezoelectric materials have the advantage that they can be converted into any shape and size. In smart textiles, polyvinylidene fluoride (PVDF) and other piezoelectric polymers are used in the form of fibers, filaments, and composites. In this research, PVDF nanofibers were developed and integrated onto a knitted fabric to fabricate a piezoelectric device for human body angle monitoring. Scanning electron microscopy and X-ray diffraction analyses were used to study the morphology and to confirm the beta phase in fibers. The results reveal that the nanofibers made from solutions with high concentration were smooth and defect-free, compared to the fibers obtained from solutions with low concentration, and possess high crystallinity as well.