Mcmahonphilipsen2642

Herein, we discovered and characterized the lasso peptide rubrinodin, which is assembled by a gene cluster encoding both an ATP-binding cassette transporter and an isopeptidase. Our bioinformatics analyses of this and other representative cluster types provided new clues into the evolutionary history of lasso peptides. Furthermore, our structural and biochemical investigations of rubrinodin permitted the conversion of this thermolabile lasso peptide into a more thermostable scaffold.Diabetic foot ulcer is a serious complication in diabetes patients, imposing a serious physical and economic burden to patients and to the healthcare system as a whole. Oxidative stress is thought to be a key driver of the pathogenesis of such ulcers. However, no antioxidant drugs have received clinical approval to date, underscoring the need for the further development of such medications. Hydrogels can be applied directly to the wound site, wherein they function to prevent infection and maintain local moisture concentrations, in addition to serving as a reservoir for the delivery of a range of therapeutic compounds with the potential to expedite wound healing in a synergistic manner. Herein, we synthesized Prussian blue nanoparticles (PBNPs) capable of efficiently scavenging reactive oxygen species (ROS) owing to their ability to mimic the activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). In the context of in vitro oxidative stress, these PBNPs were able to protect against cytotoxicity, protect mitochondria from oxidative stress-related damage, and restore nuclear factor erythroid 2-related factor 2 (NRF2)/heme oxygenase-1 (HO-1) pathway activity. To expand on these results in an in vivo context, we prepared a thermosensitive poly (d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PDLLA-PEG-PDLLA) hydrogel (PLEL)-based wound dressing in which PBNPs had been homogenously incorporated, and we then used this dressing as a platform for controlled PBNP release. The resultant PBNPs@PLEL wound dressing was able to improve diabetic wound healing, decrease ROS production, promote angiogenesis, and reduce pro-inflammatory interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels within diabetic wounds. Overall, our results suggest that this PBNPs@PLEL platform holds great promise as a treatment for diabetic foot ulcers.Partitioning gaseous water-soluble organic compounds (WSOC) to the aerosol phase is a major formation pathway of atmospheric secondary organic aerosols (SOA). However, the fundamental mechanism of the WSOC-partitioning process remains elusive. By simultaneous measurements of both gas-phase WSOC (WSOCg) and aerosol-phase WSOC (WSOCp) and formic and acetic acids at a rural site in the Yangtze River Delta (YRD) region of China during winter 2019, we showed that WSOCg during the campaign dominantly partitioned to the organic phase in the dry period (relative humidity (RH) 80%), suggesting two distinct SOA formation processes in the region. In the dry period, temperature was the driving factor for the uptake of WSOCg. In contrast, in the humid period, the factors controlling WSOCg absorption were ALW content and pH, both of which were significantly elevated by NH3 through the formation of NH4NO3 and neutralization with organic acids. Additionally, we found that the relative abundances of WSOCp and NH4NO3 showed a strong linear correlation throughout China with a spatial distribution consistent with that of NH3, further indicating a key role of NH3 in WSOCp formation at a national scale. Since WSOCp constitutes the major part of SOA, such a promoting effect of NH3 on SOA production by elevating ALW formation and WSOCg partitioning suggests that emission control of NH3 is necessary for mitigating haze pollution, especially SOA, in China.We herein introduce a lightweight and zero-power smart face mask, capable of wirelessly monitoring coughs in real time and identifying proper mask wearing in public places during a pandemic. The smart face mask relies on the compact, battery-free radio frequency (RF) harmonic transponder, which is attached to the inner layer of the mask for detecting its separation from the face. Specifically, the RF transponder composed of miniature antennas and passive frequency multiplier is made of spray-printed silver nanowires (AgNWs) coated with a poly(3,4-ethylenedioxythiophene)poly(styrenesulfonate) (PEDOTPSS) passivation layer and the recently discovered multiscale porous polystyrene-block-poly(ethylene-ran-butylene)-block-polystyrene (SEBS) substrate. Unlike conventional on-chip or on-board wireless sensors, the SEBS-AgNWs/PEDOTPSS-based RF transponder is lightweight, stretchable, breathable, and comfortable. In addition, this wireless device has excellent resilience and robustness in long-term and repeated usages (i.e., repeated placement and removal of the soft transponder on the mask). We foresee that this wireless smart face mask, providing simultaneous cough and mask-wearing monitoring, may mitigate virus-transmissive events by tracking the potential contagious person and identifying mask-wearing conditions. Moreover, the ability to wirelessly assess cough frequencies may improve diagnosis accuracy for dealing with several diseases, such as chronic obstructive pulmonary disease.Triazine UV filters are an important class of UV filters, but knowledge on their environmental occurrence and human exposure remains largely unknown. In this study, we performed a targeted analysis of 17 emerging triazine UV filters in indoor dust and indoor air from South China based on a newly developed LC-MS/MS method. A total of 12 of the 17 emerging triazine UV filters were first positively detected in the dust and air samples. Ethylhexyl triazone (EHT) and bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT) were identified as the most abundant compounds. The median total concentrations of triazine UV filters reached 3860 ng/g in indoor dust and 1590 pg/m3 in indoor air. Gas-particle partitioning analysis showed that triazine UV filters were predominant in the particle phase in ambient air. Significant concentration correlations were observed among most triazine UV filters. The estimated daily intake of triazine UV filters through dust ingestion and air inhalation for toddlers under high-end exposure scenarios was up to 839 ng/kg bw/day, but a lack of toxic thresholds hampers accurate risk assessment. Our work highlights another emerging class of UV filters that significantly contribute to indoor chemical mixtures and expresses concerns over their occurrence and human exposure.The limits of detection (LOD) and quantitation (LOQ) in the mass domain, for broadband vibrational spectroscopy with neutrons on the TOSCA spectrometer at the ISIS Pulsed Neutron and Muon Source (UK), have been studied. The well-known 3σ and 10σ approaches are used through a specifically developed analytical procedure that is based on the calculation of the integrated spectral intensities in selected energy-transfer ranges, as a function of mass of standard reference materials and calibrants, such as ZrH2, 2,5-diiodothiophene, and low-density polyethylene. The analysis shows that the blank, that is, the instrument setup without the analyte, plays a critical role in the measurement performance, especially for small specimen quantities. The results point that TOSCA enables detection of 128 μmol (LODH) and quantitation of 428 μmol (LOQH) of elemental hydrogen analytes in ZrH2. The determined values for this and other standards allow for the assessment of the calibration curve design and instrument sensitivity and define a method to be used for inelastic neutron scattering spectrometers such as TOSCA, or VESPA, the new beamline under construction at the European Spallation Source in Lund (Sweden).Surface plasmon resonance is a very well-established surface sensitive technique for label-free analysis of biomolecular interactions, generating thousands of publications each year. An inconvenient effect that complicates interpretation of SPR results is the "bulk response" from molecules in solution, which generate signals without really binding to the surface. Here we present a physical model for determining the bulk response contribution and verify its accuracy. Our method does not require a reference channel or a separate surface region. We show that proper subtraction of the bulk response reveals an interaction between poly(ethylene glycol) brushes and the protein lysozyme at physiological conditions. Importantly, we also show that the bulk response correction method implemented in commercial instruments is not generally accurate. Using our method, the equilibrium affinity between polymer and protein is determined to be KD = 200 μM. One reason for the weak affinity is that the interaction is relatively short-lived (1/koff less then 30 s). Furthermore, we show that the bulk response correction also reveals the dynamics of self-interactions between lysozyme molecules on surfaces. Besides providing new insights on important biomolecular interactions, our method can be widely applied to improve the accuracy of SPR data generated by instruments worldwide.We synthesized single crystals of Na0.55Ni6(OH)3(H0.61PO4)4 (I) and polycrystals of (Na, Ni)0.64Ni5.68(OH)3(H0.67PO4)4 (II) with ellenbergerite-like structures using the hydrothermal method. The phases crystallize in the hexagonal space group P63mc with the following unit cell parameters a = 12.5342(1) Å, c = 4.9470(1) Å, and V = 673.08(2) Å3 for I; a = 12.4708(2) Å, c = 4.9435(2) Å, and V = 665.82(2) Å3 for II; and Z = 2. Y27632 Their crystal structures are based on a 3D framework built from NiO6 octahedra and PO4 tetrahedra. The difference between I and II lies in the way the structural channels are filled along the [001] direction. These channels accommodate segments of Na- and (Na, Ni)-centered chains of face-sharing octahedra in the structures I and II, respectively. The magnetic susceptibility χ and the specific heat Cp evidence pronounced low-dimensional magnetic behavior at elevated temperatures and the formation of the weakly ferromagnetic long-range order at TNI = 61 K and TNII = 63 K. Analysis of the χ(T) data within both chain and dimer spin models allows the estimation of the leading exchange interaction parameters in the compounds under study.SiOx-based anode materials are considered to be promising and have been gradually commercialized due to their high specific capacity as well as the acceptable volume change during lithiation/delithiation and preferable cycling stability compared to that of Si. Nevertheless, their inherently low Coulombic efficiency hinders the large-scale application. Up to now, researchers have paid much attention to the initial Coulombic efficiency and developed a series of effective prelithiation strategies. However, the subsequent cycles (focusing on the 2nd to 10th), during which the SiOx anode suffers great lithium consumption as well, have received scarcely any concerns. In this work, a strategy of high-temperature (50 °C) initial charge after an overcapacity prelithiation for a SiOx-based full-cell battery is proposed. As high temperature can promote the reaction between lithium and the SiO2 matrix of SiOx, SiO2 will experience a one-step thorough reduction rather than gradual conversion in subsequent cycles, improving the subsequent Coulombic efficiencies (SCEs) accordingly.