Mcintyreterp6819

Background Because primary squamous cell carcinoma (SCC) of the breast is a rare disease, the standard therapy has not been established. We examined the clinical outcomes of postoperative adjuvant radiotherapy for breast SCC. Material and methods We conducted a multicenter retrospective cohort study. Patients diagnosed with primary breast SCC who received adjuvant radiotherapy as part of their primary definitive treatment were included. Overall survival (OS), breast cancer-specific survival (BCSS), and recurrence-free interval (RFi) were evaluated. Results Between January 2002 and December 2017, 25 breast SCC patients received adjuvant radiotherapy as a primary treatment were included. Median follow-up time was 43.5 months. Three (12%), fifteen (60%) and seven (28%) patients had clinical stage I, II and III disease, respectively. Fourteen patients underwent breast-conserving surgery and subsequent adjuvant radiotherapy. Eleven patients underwent mastectomy and post-mastectomy radiotherapy. Ten patients received regional lymph node irradiation. Nine (36%) patients had disease recurrence. The first site of recurrence was locoregional in five, but distant metastasis arose in one. Concurrent local and distant metastasis were seen in two. Six cases of local recurrence occurred within the irradiated site. Seven patients died, and six of the deaths were due to breast cancer. Five-year OS, BCSS, and Rfi were 69%, 70%, and 63%, respectively. In multivariate analysis, age and lymphatic invasion were associated with increased risk of recurrence. Conclusion Breast SCC has a high incidence of locoregional recurrence and poor prognosis. Age and lymphatic invasion are significant risk factors for recurrence.The aim of the presented work is to compare two popular chemometric methods which are partial least squares regression (PLSR) and support vector regression (SVR). The comparison shows their characteristics via application of the suggested methods to analysis of Norfloxacin (NF) and Tinidazole (TZ) with the presence of a potential impurity of Tinidazole; 2-Methyl-5-nitro-1H-imidazole (MNZ). For appropriate analysis, a 3 factor 4 level experimental design was constructed, which results in a training set composed of 16 mixtures which contains different concentrations of the three components; achieving symmetry, rotatability and orthogonality in mixture space. In order to validate the prediction ability of the suggested models, an independent test set consisting of 8 in-space and 8 out-of-space mixtures was used. The presented results show high specificity and accuracy of the mentioned multivariate calibration models for analysis of in-space samples of NF and TZ in presence of (MNZ) using UV spectral data. Statistical comparisons of predictive abilities of proposed models against classical least squares CLS model and against each other was performed; whether for analysis of test set mixtures or dosage form. CLS model showed lower predictive ability compared to other models. Results obtained by SVR model are as accurate as PLSR model, however, optimization and implementation of PLSR is faster and easier, hence PLSR could be of choice for this given case study. The developed chemometric models were validated as directed by ICH strategies. The validated methods were efficiently used for estimation of NF and TZ in pure powders and pharmaceuticals which indicates their suitability for application in quality control examination of both of the drugs.One of the medical problems is premature ejaculation which characterized by quick ejaculation and reaching orgasm rapidly. Dapoxetine was accepted as off-label antidepressant used in the treatment of premature ejaculation, so hereby we provide an innovative, non-extractive, environmentally safe protocol for the assay of dapoxetine in biological plasma and tablet. The principle of the assay is simple and only based on native fluorescence which was enhanced by micelle. Parameters influencing the method were optimized and measurements were accomplished at emission wavelength of 338 nm after excitation at 294 nm. The fluorescence-concentration plot was rectilinear over the range of 0.1-4 μg/mL. Directives of ICH guideline were the rules which followed to ensure the validity of the work. Eventually, the procedure was utilized in the dosage form assay and extended to include biological plasma analyses, with good percentage recuperation.Diazinon is a typical phosphorothionate, which is widely used to prevent and control harmful organisms that endanger the agriculture productions. However, it is among the most toxic substances and can cause damage to the environment, food and human health even in very low concentrations. Hence, ultra-sensitive screening methods are urgently required for the detection of this extensively used pesticide. In this study, a rapid and sensitive fluorescence resonance energy transfer (FRET) method was developed for low concentration detection of diazinon in food. The aptamer-modified upconversion nanoparticles (Apt-UCNPs) were synthesized and conjugated with graphene oxide (GO) through π-π interaction. Due to the FRET between UCNPs and GO, the fluorescence was quenched. When diazinon was added, the aptamer preferentially bound with it, caused the separation of GO, and resulted in the enhancement of fluorescent signal. Under the optimal conditions, a wide linear detection range from 0.05 to 500 ng/mL was achieved, with a limit of detection (LOD) of 0.023 ng/mL. The proposed method was successfully applied to measure diazinon in real samples. Results showed that the proposed nanosensor offers an efficient, specific and simple approach for the detection of diazinon in food and has a high potential for food safety and quality control.In this paper, nanoparticles decorated Ce2(WO4)3 nanosheets (CWNSs) with negative potential and large specific surface area were synthesized and developed as highly efficient peroxidase mimics for colorimetric detection of H2O2. CWNSs can efficiently catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to induce an obvious color variation. Kinetic analysis indicated that the catalytic behaviors of CWNSs obey the typical Michaelis-Menten mechanism. check details The peroxidase-like catalytic mechanism of CWNSs was proposed according to the active species trapping experiments, verifying that ·O2- radicals played primary roles in the catalytic reaction. Based on the strong and stable peroxidase-like catalytic activity of CWNSs, a simple, rapid, selective, and ultrasensitive method was successfully established for colorimetric detection of H2O2. The method has a good linear response ranging from 0.5 μM to 100 μM for H2O2 concentration with a lower detection limit of 0.15 μM. Benefitting from the sensitive response and good stability, the method is applied in real sample detection and shows a favorable reproducibility and feasibility.