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The environmental data we collected (sampling depth, sampling location latitude and longitude, organic carbon content, and organic nitrogen content in the sediment) had no significant influence on the composition and distribution of fungal communities. Our findings provide valuable information for understanding the distribution and potential ecological functions of fungi in the deep-sea sediments of the Magellan seamounts.Vast parts of the Baltic Sea have been mycologically neglected and are still awaiting exploration. Here we summarise earlier records of marine fungi from the Baltic, supplementing them with discoveries from fieldwork in Sweden in 2019. Although marine fungal diversity is clearly attenuated in the brackish water of the Baltic Sea, a substantial number has still been discovered. Here we list 77 species from the Baltic Sea, whereas after a critical assessment a further 18 species have been excluded as records of marine fungi. The species have mainly been identified by their morphological features, supplemented by DNA-based diagnostics. Most of the species have their main distributions in temperate areas of the Atlantic Ocean. Some of the Baltic species discovered here represent far disjunctions to tropical waters while only a very few are until now only recorded for the Baltic Sea. In this paper two species belong in Basidiomycota, while the most ascomyceteous speciose classes are Sordariomycetes (with 42 species) and Dothideomycetes (24). Halosphaeriaceae is the most speciose family in marine habitats, as also in the Baltic Sea, represented here by 29 species. Three species are new to Europe, and in addition 13 to the Baltic Sea and 13 to Sweden.Sea turtles are presently considered severely endangered species that are historically threatened by many environmental factors. Recently, additional threats to sea turtles from two pathogenic species of fungi in the Fusarium solani species complex (F. read more falciforme and F. keratoplasticum) have been identified. These species infect marine turtle eggs, causing sea turtle egg fusariosis, and kill their embryos, with recent reports of hatch-failure in seven globally distributed species of endangered sea turtles (Caretta caretta, Chelonia mydas, Dermochelys coriaceae, Eretmochelys imbricata, Lepidochelys olivacea, Lepidochelys kempi and Natator depressus). Mycelia and spores of pathogenic species of Fusarium are produced in disturbed terrestrial soils and are transported to the ocean in coastal run off. We propose that these fungi grow on floating particles of plant tissues (leaves and wood), animal tissues, silt and plastics, which are carried by wind and currents and the turtles themselves to the beaches where the turtles lay their eggs.This study introduces a novel holomorphic marine fungal species, Halobyssothecium estuariae (Lentitheciaceae, Pleosporales), from dead Phragmites communis. The new species has semi-immersed, subglobose or ellipsoidal, papillate, conical ascomata, clavate to subcylindrical, short pedicellate asci and 3-septate, fusoid to ellipsoidal ascospores with rounded ends, pale brown to dark brown central cells and hyaline end cells. The asexual morph has multiseptate, filiform, intercalary, catenate, branched chlamydospores that resemble Xylomyces. The asexual morph of Keissleriella phragmiticola based on combined LSU, SSU, ITS and TEF1 sequence analyses is reported. The role of molecular identification in delineating cryptic species are also discussed.

Dietary diversity is defined as the number of food groups or items consumed over a reference period, and usually, it is a problem in developing countries including Ethiopia. Inadequate dietary diversity is one of a major public health problem and can result in physical, emotional, and psychological changes among adolescents. However, studies on dietary diversity among school children were very limited. Hence, this study aimed at determining dietary diversity practices and factors among adolescents in Dembia district.

A school-based cross-sectional study was conducted from March 1 to April 15, 2017, at Dembia district, northwest Ethiopia. A total of 474 study subjects were selected using the multi-stage sampling technique. A structured and pre-tested questionnaire was used to collect the data. Dietary diversity was measured through standard tool adopted from Food and Nutrition Technical Assistance (FANTA) 2016 using the 24-h recall method. A multivariable binary logistic regression model was employed to idactice of adolescents by giving due attention to poor households and undernourished adolescents.One of the most devastating pests of chili pepper is the sweet potato whitefly (SPW), Bemisia tabaci (Gennadius) (Hemiptera Aleyrodidae). It sucks plant sap, emits honeydew on which sooty mold fungi grow, and transmits the pepper yellow leaf curl Indonesia virus (PepYLCIV), the most damaging viral disease of chili in Indonesia. Farmers rely mainly on insecticide to control the insect with two to three sprays in a week. To reduce the insecticide use, an integrated approach needs to be developed. Therefore, the current study evaluated the effectiveness of the integration of reflective mulch, host plant resistance, and insecticide use for managing the sweet potato whitefly on the chili pepper. In 2018, a complete randomized block design was used in three separate trials to assess the effects of mulch, cultivar, or insecticide application frequency on the numbers of whitefly eggs, nymphs, and adults. In 2019, a split-split plot design was used to evaluate the effects of the integration of mulch, cultivar, and insecticide application frequency on the numbers of whitefly eggs, nymphs, and adults. The results showed that the reflective silver mulch had significantly lower numbers of whitefly eggs, nymphs, and adults, in comparison to the rice straw mulch and bare ground treatments. Chili plants cv. Bara was more resistant than Bhaskara against B. tabaci in the field; however, in the no-choice trial, no significant difference was detected between both cultivars. Insecticide applications twice per week and once per week were equally effective in controlling the whitefly on the susceptible cultivar (Bhaskara). Overall, the integration of reflective mulch, resistant cultivar, and insecticide application every two weeks effectively suppressed B. tabaci populations on the chili pepper. This approach could substantially reduce the number of insecticide applications from twice per week (commonly practiced by chili farmers in the area) to one application only in two weeks.Nipa palms, a plant species in mangrove forest, are valuable and beneficial for the local community's economy as well as the conservation in the southern region of Thailand. This study aimed to investigate the use of nipa palms in Khanap Nak and focused on the type of products made from nipa palms and the yield of this plant through focus group discussion and interview with the farmers maintaining nipa palm forest. The results suggested that nipa palms in Khanap Nak can yield for 5-100 years. Apart from the benefits to the community in terms of sustainability, as it prevents erosion, it provides sources for the production of food (molasses, granulated sugar, syrup, and vinegar from sap) and raw materials (roofing material and cigarette paper from leaves and stem), which can generate income to community members. It was found that most of the communities earn 90-130 USD/day from palm sugar production. The quantity of nipa palm products varies in each season, resulting in different prices. In this regard, they can produce high quantity of products made from sap from January to March. However, the production of different types of sugar requires local knowledge and wisdom to ensure good quality. Nipa palm production is the sustainable way to utilize mangrove forest resources, leading to effective conservation and good life quality. Regarding problems and difficulties in farming, it was found that natural disaster is a major threat, such as drought, excessive amount of salt or freshwater in certain periods, and insect pests.

Hemophilia A is an X-linked recessive bleeding disorder caused by mutations in FVIII gene with an incidence of 1 in 5,000 to 10,000 live born males. The Inv22 mutation is a major cause of the disease worldwide, accounting for up to 40%-50% of severe FVIII mutations. The aim of the present study was to screen Inv22 of the FVIII gene in Palestinian patients with severe HA and reveal its role as a predisposing factor for the development of inhibitors.

A cohort of 77 HA individuals including 5 carrier females from 52 unrelated families registered at governmental hemophilia centers in the West Bank area of Palestine was investigated. The demographic data and the clinical history were retrieved from medical files. Molecular analysis of Inv22 mutation in severe HA (30 cases) from Palestine was performed using the subcycling polymerase reaction (S-PCR). FVIII coagulant activities were carried out on an aPTT-based 1-stage clotting assay. FVIII inhibitors were quantified using the Nijmegen modification of the Bethes a simple, rapid, and cost-effective method for the diagnosis of Inv22 in severe HA patients. Although the Inv22 mutation was associated with 36.6% of severe HA phenotype cases, it was not a major predisposing factor for inhibitor formation.In this study, high-performance liquid chromatography (HPLC) and colorimeter were applied to evaluate the quality of different species and differently prepared slices of Zedoray Rhizome samples with the aid of chemometric tools. Fifty batches of Zedoray Rhizome samples from different species and forty-two batches of Zedoray Rhizome samples from differently prepared slices were collected. The quantitative method was developed using HPLC to simultaneously determine the contents of twelve chemical ingredients in Zedoray Rhizome. The colour parameters L, a, and b were measured by a colorimeter. Then, the collected data were analyzed by the principal component analysis and Pearson correlation analysis. The results showed that the proposed method was capable of accurately determining the contents of the twelve chemical ingredients and the colour parameters for the collected samples. There was a dramatic difference in the contents of the chemical ingredients and in the colour parameters among different species and differently prepared slices of Zedoray Rhizome samples. This study reveals that combining HPLC, colorimeter, and chemometric tools can provide a new approach to comprehensively evaluate the quality of Zedoray Rhizome samples.A preferable approach of a combination of a multiwavelength fusion HPLC fingerprint and chemometrics for the quality control of Xiaoer Chiqiao Qingre granules (XCQG) was established in this study. A single-wavelength HPLC fingerprint was performed to identify 18 peaks as common peaks in the beginning, and 12 of them were recognized by HPLC-Q/TOF-MS. To overcome the limitation of the single-wavelength HPLC fingerprint, a three-wavelength (230 nm, 250 nm, and 330 nm) fusion fingerprint was established for a more thorough quality assessment. Six main active ingredients (geniposide, paeoniflorin, forsythin, forsythoside A, baicalin, and wogonoside) were selected as chemical markers for simultaneous quantitative analysis, while the results indicated that the content of other five ingredients except forsythoside A presented comparatively stable. Chemometrics including hierarchical cluster analysis (HCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to evaluate the homogeneity and heterogeneity of sixteen batches of XCQG.

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