Mcallistermagnussen4034

Catalytic conversion of lignin to certain aromatic compounds has been extensively studied but still has great challenges. Photocatalytic depolymerizing lignin is a very promising method to obtain valuable chemicals. CB-5083 in vivo Herein, Zn4In2S7 (ZIS)-based photocatalyst was successfully synthesized by simply combining ZIS and graphene oxide (GO). Photocatalyst ZIS-100 can efficiently depolymerize organosolv lignin into phenols and ketones. The relative content of valuable compounds in the depolymerized product was increased by 2.5 times as compared that without photocatalyst. The photocatalyst can effectively break Cβ-O bonds in 2-phenoxy-1-phenylethanol (PP-ol, a model compound) and the conversion of PP-ol is 93.27%. Mechanism studies show that the thiol groups on the surface of ZIS-100 play an important role in the formation of Cα radical intermediates. Photocatalytic cleavage of Cβ-O bond mainly follows a one-step reaction mechanism through a self‑hydrogen transfer process. This study provides a new strategy for selectively breaking Cβ-O bond in lignin to form valuable chemicals.Plasmodium falciparum expresses two essential cytosol localised chaperones; PfHsp70-1 and PfHsp70-z. PfHsp70-z (Hsp110 homologue) is thought to facilitate nucleotide exchange function of PfHsp70-1. PfHsp70-1 is a refoldase, while PfHsp70-z is restricted to holdase chaperone function. The structural features delineating functional specialisation of these chaperones remain unknown. Notably, PfHsp70-z possesses a unique linker segment which could account for its distinct functions. Using recombinant forms of PfHsp70-1, PfHsp70-z and E. coli Hsp70 (DnaK) as well as their linker switch mutant forms, we explored the effects of the linker mutations by conducting several assays such as circular dichroism, intrinsic and extrinsic fluorescence coupled to biochemical and in cellular analyses. Our findings demonstrate that the linker of PfHsp70-z modulates global conformation of the chaperone, regulating several functions such as client protein binding, chaperone- and ATPase activities. In addition, as opposed to the flexible linker of PfHsp70-1, the PfHsp70-z linker is rigid, thus regulating its notable thermal stability, making it an effective stress buffer. Our findings suggest a crucial role for the linker in streamlining the functions of these two chaperones. The findings further explain how these distinct chaperones cooperate to ensure survival of P. falciparum particularly under the stressful human host environment.Strong inhibition of the human UDP-glucuronosyltransferase enzymes (UGTs) may lead to undesirable effects, including hyperbilirubinaemia and drug/herb-drug interactions. Currently, there is no good way to examine the inhibitory effects and specificities of compounds toward all the important human UGTs, side-by-side and under identical conditions. Herein, we report a new, broad-spectrum substrate for human UGTs and its uses in screening and characterizing of UGT inhibitors. Following screening a variety of phenolic compound(s), we have found that methylophiopogonanone A (MOA) can be readily O-glucuronidated by all tested human UGTs, including the typical N-glucuronidating enzymes UGT1A4 and UGT2B10. MOA-O-glucuronidation yielded a single mono-O-glucuronide that was biosynthesized and purified for structural characterization and for constructing an LC-UV based MOA-O-glucuronidation activity assay, which was then used for investigating MOA-O-glucuronidation kinetics in recombinant human UGTs. The derived Km values were crucial for selecting the most suitable assay conditions for assessing inhibitory potentials and specificity of test compound(s). Furthermore, the inhibitory effects and specificities of four known UGT inhibitors were reinvestigated by using MOA as the substrate for all tested UGTs. Collectively, MOA is a broad-spectrum substrate for the human UGTs, which offers a new and practical tool for assessing inhibitory effects and specificities of UGT inhibitors.Sedum sarmentosum Bunge (SS) is clinically used as Chinese medicine for hepatitis related diseases treatment. The purpose of this study was to explore the chemical structures of polysaccharides from this plant. A neutral polysaccharide (SSWP) was isolated and purified by ion-exchange chromatography and Superdex-75 column. The obtained SSWP was a homogenous one with a molecular weight of 21.5 kDa according to the high-performance gel permeation chromatography. The major monosaccharide composition of SSWP was arabinose, glucose and galactose in a molar ratio of 2.411.8. The methylation analysis showed that SSWP consists mainly of Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, →4)-Galp-(1→, →4)-Glcp-(1→. The NMR result and enzymatic digestion data comprehensively indicated that SSWP was a novel arabinogalactoglucan-type structure. The anticancer assay in vitro exhibited that SSWP could effectively inhibit 48.9% of Huh-7 cells growth at 50 μg/mL and arrest cells at S-phase, and induce tumor cells apoptosis. Together, polysaccharide from S. sarmentosum Bunge could be a potential natural antitumor agent.The pathways linking giving and receiving emotional and instrumental social support, and cardiovascular reactivity (CVR) are not yet fully understood. Eight-two healthy young adults completed psychometric measures of giving and receiving emotional and instrumental social support and participated in a standardised laboratory stress task. Cardiovascular and hemodynamic parameters were monitored throughout. Both giving and receiving emotional support were positively associated with systolic blood pressure (SBP) and diastolic blood pressure (DBP), such that those reporting giving and receiving more emotional support had higher reactivity. Only receiving instrumental was associated with DBP, with those receiving more instrumental support having higher reactivity. Moreover, while the significant association between giving social support and CVR withstood adjustment for several confounding factors (e.g., BMI, sex) it was abolished when receiving support was controlled for. These findings are novel and extend the literature on social support and CVR.