Martinovesen5398
To explore the role and of miR-132, HMGA2 and PI3K/AKT pathway in mice with Alzheimer's disease (AD).
The mice were divided into 7 groups the normal group, the model group (AD model mice), the NC group (AD mice injected with negative control (NC) vector), the miR-132 mimic group (AD mice injected with miR-132 mimics), the miR-132 inhibitor group (AD mice injected with miR-132 inhibitor), the si-HMGA2 group (AD mice injected with HMGA2 silencing vector), and the miR-132 inhibitor + si-HMGA2 group (model mice treated with miR-132 inhibitor and si-HMGA2). Y-maze experiment and related molecular biology experiments were performed.
The double-luciferase reporter assay verified that miR-132 could target and inhibit the expression of HMGA2A. Compared with the NC group, model mice had decreased learning and memory ability, reduced miR-132, p-PI3K/PI3K, p-AKT/AKT, AQP4 expression as well as GFAP GSH-Px, SOD, ATP, and T-AOC levels, but increased expression of HMGA2 and the levels of TNF-α, IL-6, NO, IL-1β, MAO, and MDA (P<0.017). Up-regulation of miR-132 or silencing HMGA2 could partly reverse the changes, but inhibition of miR-132 would exaggerate the brain injury and these molecular changes (P<0.017). The combination uses of si-HMGA2 and miR-132 inhibitor could reverse the changes caused by miR-132 inhibitor (P<0.017).
miR-132 could downregulate the expression of HMGA2 and promote the expression of the PI3K/AKT pathway, so as to achieve a protective effect on brain in AD mice.
miR-132 could downregulate the expression of HMGA2 and promote the expression of the PI3K/AKT pathway, so as to achieve a protective effect on brain in AD mice.
This study explored and analyzed the effects of targeted regulation of LATS2 by LncRNA BCAR4 on the proliferation, migration and apoptosis of hepatocellular carcinoma (HCC).
We detected the expression of LncRNA, BCAR4 and LATS2 mRNA in liver hepatocellular carcinoma HepG2 cells and normal hepatocellular cells LO2 by RT-PCR. HepG2 cells were divided into BCAR4-siRNA, NC-siRNA and control groups. We detected the targeted regulation of LncRNA BCAR4 on LATS2 by luciferase gene assay, and measured the proliferation, migration and apoptosis of cells in each group by RT-PCR, MTT, Transwell and flow cytometry, respectively.
The relative expression of LncRNA BCAR4 in HepG2 cells was critically higher than that in LO2 cells (
), while LATS2 mRNA in HepG2 cells was significantly less than that in LO2 cells (
). selleckchem Compared with NC siRNA group, the content of luciferase in BCAR4 siRNA group was much higher (
); The relative expression of LncRNA BCAR4 in BCAR4 siRNA group decreased dramatically than that in NC-siRNAliver cancer.
To inquire into the significance of coagulation indexes in the progression of cirrhosis.
A total of 108 patients with cirrhosis treated in our hospital were collected as the research group (RG), and 105 healthy people who underwent concurrent physical examination were selected as the control group (CG). The coagulation indexes of all the participants were tested to determine their significance in cirrhosis progression.
Compared with the CG, prothrombin time (PT), activated partial thrombin time (APTT) and thrombin time (TT) in the RG were statistically prolonged, while fibrinogen (FIB) was notably decreased (P<0.05). With the increase of Child-Pugh score, PT, APTT and TT prolonged and FIB reduced gradually (P<0.05). The coagulation indexes of patients were correlated with Child-Pugh score (P<0.05). Patients in the RG showed markedly higher alanine aminotransferase (ALT), total bilirubin (TBil), total bile acid (TBA), mean platelet volume (MPV), platelet distribution width (PDW) and platelet-larry, and can provide evidence for the early diagnosis of cirrhosis patients, with clinical significance.
Liver cancer is a common cancer that enormously threatens the health of people worldwide. With the continuous advances of high-throughput gene sequencing technology and computer data mining technology, researchers can understand liver cancer based on the current accumulation of gene expression data and clinical information.
We downloaded the TCGA data of liver cancer on the cancer-related website (https//genome-cancer.ucsc.edu/proj/site/hgHeatmap/), comprising 438 patients and 20,530 genes. After removing some patients with missing survival data, we collected 397 patients' samples. Our data were collected from a public database without real patient participation. While matching the patient samples in the gene expression spectrum, we attained 330 samples with primary tumors and 50 samples with normal solid tissue.
After the 330 tumor tissue samples were randomized into two equal-numbered groups (one is a training set, and the other is a test set), we selected 26 gene biomarkers from the training set and validated them in the test set. Based on the selected 26 gene biomarkers, RBM14, ALG11, MAG, SETD3, HOXD10 and other 26 genes were considered independent risk factors for the prognosis of liver cancer, and genes such as GHR significantly affect human growth hormone for liver cancer. The findings discovered that low-risk patients survived remarkably better than the high-risk patients (P<0.001), and the area under the curve (AUC) of receiver operating characteristic curve (ROC) was greater than 0.5.
Our numerical results showed that these 26 gene biomarkers can be used to guide the effective prognostic therapy of patients with liver cancer.
Our numerical results showed that these 26 gene biomarkers can be used to guide the effective prognostic therapy of patients with liver cancer.
Traditional Chinese medicine has been increasingly used in the prevention and treatment of gastric cancer, especially in application of compound Chinese medicine. The aim of this study was to investigate the effect of Qi Ling decoction (QLD) on the invasion and metastasis of gastric cancer and its related signaling pathways at the cellular and molecular level in vitro, and explore the mechanism of QLD.
Scratch assay, transwell assay, and adhesion experiments were used to study the effects of QLD and its compounds on gastric cancer. Western blot was employed to detect expression of the PI3K/Akt pathway after administration of QLD.
QLD can significantly inhibit the invasion, migration, and adhesion of gastric cancer cells in vitro. The main chemical components of QLD (diosgenin, catechins, and calycosin) can also inhibit the invasion, migration and adhesion of gastric cancer cells. Furthermore, QLD inhibits MMP-9 and affects gastric cancer cell metastasis through the PI3K/Akt pathway.
QLD and its three main chemical components can inhibit the invasion, migration, and adhesion of gastric cancer cells, and the mechanism may be related to the PI3K/Akt pathway.
