Martinedvardsen1713

Recent reports suggest SARS-CoV-2, the virus causing COVID-19, may be transmittable from pregnant mother to placenta and fetus, albeit rarely. The efficacy of vertical transmission of SARS-CoV-2 critically depends on the availability of its receptor, ACE2, in the placenta. In the present study, we tested the hypothesis that placental ACE2 expression is oxygenation-dependent by studying the expression of ACE2 and associated cell entry regulators in the monochorionic twin anemia-polycythemia (TAPS) placenta, a model of discordant placental oxygenation.

We performed a retrospective comparative immunohistochemical, immunofluorescence and Western blot analysis of ACE2, TMPRSS2 and Cathepsin B expression in anemic and polycythemic territories of TAPS placentas (N=14).

ACE2 protein levels were significantly higher in the anemic twin territories than in the corresponding polycythemic territories, associated with upregulation of the key ACE2-related cell entry regulators, TMPRSS2 and Cathepsin B, immunolocalized to villous trophoblastic and stromal cells. Cellular colocalization of ACE2 and TMPRSS2, suggestive of functionality of this cell entry axis, was demonstrated by double immunofluorescence studies.

Placental hypoxia is associated with upregulation of ACE2 expression, concomitant with increased expression of its key cell entry proteases. ACE2-regulated placental functions, both infection- and non-infection related, may be highly oxygenation-dependent.

Placental hypoxia is associated with upregulation of ACE2 expression, concomitant with increased expression of its key cell entry proteases. ACE2-regulated placental functions, both infection- and non-infection related, may be highly oxygenation-dependent.

Chronic abruption oligohydramnios sequence (CAOS) is histologically characterized by diffuse chorioamniotic hemosiderosis (DCH). However, the criteria for the histological evaluation of the extent of CAOS-related hemosiderin deposition (HD) of the membranes and the difference in HD between the chorionic plate (CP) and fetal membrane (FM) are not well studied. This case control study compared the degree and distribution pattern of HD on CP and FM to present the histological features of DCH and the criteria for histological evaluation.

From the medical records of Kyoto University Hospital (2010-2019), we selected 20 CAOS cases that were clinically diagnosed by Elliot's criteria. Twenty non-CAOS cases matched to the CAOS group by gestational age were selected as controls. We compared the clinical data and pathological features in the two groups. We performed iron staining in all the cases and analyzed HD in CP and FM according to the histological score (H-Score 0-12), which was determined as the density (0-3) multiplied by the extent of staining (0-4).

HD was found in 100% (20/20) of CAOS and 15% (3/20) of control cases. In both the FM and CP, CAOS cases showed a significantly higher HS than control cases (CAOS, HS=4-12; Control, HS=0-1, p<0.0001). Three CAOS patients presented HD alone in the CP. The HS of the CP was significantly higher than that of the FM (p=0.0003).

CAOS presented DCH with HS≥4. This study showed that the CP might be more suitable for evaluating DCH than the FM.

CAOS presented DCH with HS ≥ 4. This study showed that the CP might be more suitable for evaluating DCH than the FM.

The purpose of this study was to examine the health-related quality of life (HRQOL) of female children with CAH as reported by children and their caregivers.

A convergent mixed methods design was selected whereby quantitative and qualitative results were merged to provide a comprehensive understanding of HRQOL of children. Semi-structured interviews were conducted with 20 child-caregiver dyads. The full sample of child-caregiver dyads (N=25) completed KINDL-R questionnaires, which provided a quantitative measure of children's HRQOL.

Children and their caregivers reported good overall HRQOL. Children scored significantly lower on the KINDL-R School subscale compared to their caregivers. Associations were observed between the HRQOL score from one graphic rating scale item and the child's other health issues and child's diagnosis. Themes emerging from the child and caregiver interviews were health-related quality of life, impact of stigma on psychological well-being, information-sharing and disclosure of CAH, and improving the quality of life of children with CAH.

This mixed methods study provided evidence to understand the health and complex needs of children with CAH.

Clinicians may better support children and caregivers by expanding the focus beyond medication management to include 1) psychological support and resources (i.e., developmentally appropriate coping and adaptation strategies); 2) continuous education for clinical staff, school nurses, emergency medical transport staff, and providers; and 3) public awareness beyond the clinic and hospital settings.

Clinicians may better support children and caregivers by expanding the focus beyond medication management to include 1) psychological support and resources (i.e., developmentally appropriate coping and adaptation strategies); 2) continuous education for clinical staff, school nurses, emergency medical transport staff, and providers; and 3) public awareness beyond the clinic and hospital settings.Recently, the COVID-19 disease spread has emerged as a worldwide pandemic and cause severe threats to humanity. The World Health Organisation (WHO) releases guidelines to help the countries to reduce the spread of this virus to the public, like wearing masks, hand hygiene, social distancing, shutting down all types of public transports, etc. These conditions led to a worldwide economic fall drastically, and on the other hand, indirect environmental benefits like global air quality improvement and decreased water pollution are also pictured. Currently, use of face masks is part of a comprehensive package of the prevention and control measures that can limit the spread of COVID-19 since there is no clinically proven drugs or vaccine available for COVID-19. Mostly, face masks are made of petroleum-based non-renewable polymers that are non-biodegradable, hazardous to the environment and create health issues. This study demonstrates the extensive use of the face mask and how it affects human health and the marine ecosystem. It has become a great challenge for the government sectors to impose strict regulations for the proper disposal of the masks as medical waste by the public. Neglecting the seriousness of this issue may lead to the release of large tonnes of micro-plastics to the landfill as well as to the marine environment where mostly end-up and thereby affecting their fauna and flora population vastly. Besides, this study highlights the COVID-19 spread, its evolutionary importance, taxonomy, genomic structure, transmission to humans, prevention, and treatment.

Cardiac hypertrophy and fibrosis are closely related to cardiac dysfunction, especially diastolic dysfunction. Limited medications can be used to simultaneously delay cardiac hypertrophy and fibrosis in clinical practice. Piperlongumine (PLG) is an amide alkaloid extracted from Piper longum and has been shown to have multiple biological effects, including anticancer and antioxidant effects. However, the role of PLG in cardiac hypertrophy and fibrosis is not clear.

The aim of this study was to reveal the role of PLG in cardiac hypertrophy and fibrosis and the associated mechanism.

Cardiac hypertrophy and fibrosis were induced by angiotensin II (Ang II) in vivo and in vitro. The effect of PLG in vivo, in vitro and its mechanism were investigated by proliferation and apoptosis assays, western blot, real-time PCR, immunofluorescence, histochemistry, echocardiography, flow cytometry and chromatin immunoprecipitation.

Proliferation and apoptosis assays showed that 2.5μM PLG slightly inhibited proliferation G in both cardiac hypertrophy and fibrosis and the potential value of PLG for developing novel medications for pathological cardiac hypertrophy and heart failure.Poly-3-hydroxybutyrate is a biopolymer which has shown tremendous potential for replacing conventional petroleum-based plastics for plummeting the plastic pollution problem. However, the production cost of PHB is high which makes it less attractive for commercial use. To tackle this challenge, various researchers suggest the search for low-cost substrates and energy efficient technologies for PHB production. In this regard, the waste generated from fruit processing industries or fruit wastes could be pre-processed and fermented for effectively generating PHB. Selleckchem Elesclomol Therefore, the aim of this review was to focus on the methods of fruit waste pre-processing and the effect of fermentation variables on PHB production using fruit waste as a substrate. The relevant research findings on the use of different microorganisms, PHB production conditions and fruit waste-based substrates are also covered. Analysis of various studies revealed that pineapple and mixed fruit waste are effective for PHB production.The key step for anaerobic biodegradation of 2,4-dichlorophenol (2,4-DCP) is an initial dechlorination reaction, but Cl in the para-position is more difficult to remove than Cl in the ortho-position using normal 2,4-DCP-acclimated bacteria. In this work, a bacterial community previously acclimated to biodegrading 2,4-DCP slowly dechlorinated 4-chlorophenol (4-CP Cl only in the para-position), which limited mineralization. That community was exposed to the selective pressure of having 4-CP as its only organic substrate in order to generate a 4-CP-dechlorinating community. When the 4-CP-dechlorinating community was challenged with 2,4-DCP, 4-CP hardly accumulated, although the kinetics for 2,4-DCP biodegradation were slower. When the community acclimated to 4-CP was mixed with the community acclimated to 2,4-DCP, the 2,4-DCP removal rate remained high, and 4-CP was more rapidly biodegraded. The genera Treponema, Blvii28, Dechloromonas, Nitrospira, and Thauera were significantly more abundant in the 4-CP-dechlorinating biomass and may have played roles in 2,4-DCP dechlorination and mineralization.Excess nitrogenous compounds in municipal or industrial wastewaters can stimulate growth of denitrifying bacteria, in return, to convert potentially hazardous nitrate to inorganic nitrogen gas. To explore the community structure, distributions and succession of functional strains, and their interactions with other microbial communities, contemporary studies were performed based on detailed genomic analysis. This mini-review updated contemporary genomic studies on denitrifying genes in natural and engineered aquatic systems, with the constructed wetlands being the demonstrative system for the latter. Prospects for the employment of genomic studies on denitrifying systems for process design, optimization and development of novel denitrifying processes were discussed.We report results of the studies related to the fabrication of thionine functionalized graphitic carbon nitride nanosheets based ultrasensing platform for food toxin (Aflatoxin B1, AfB1) detection. The synthesis of graphitic carbon nitride nanosheets (g-C3N4) was carried out by polycondensation of melamine followed by chemical exfoliation. Further, thionine was used for the functionalization of g-C3N4 (Thn/g-C3N4) and deposited electrophoretically onto the indium tin oxide (ITO) coated glass electrode. The fabricated Thn/g-C3N4/ITO electrode was covalently immobilized by EDC-NHS chemistry with anti-aflatoxin B1 (anti-AfB1) followed by blocking of non-specific sites using BSA molecules. For structural, morphological, functional and electrochemical properties analysis of synthesized nanomaterials and fabricated electrodes X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, atomic force microscopy and cyclic voltammetry techniques were used.