Maddenbass0493

Our research has provided a reliable tool and strategy for visual detection of As(iii) with outstanding sensing ability.Cobalt is an essential element for living systems, which, however, make very limited use of this metal, using it mainly in cobalamin-containing enzymes. The reduced use of cobalt compared to other transition metals is generally attributed to the potential toxicity of this element. In this work, we demonstrate that cobalt not only does not have an obvious toxic effect on Salmonella Typhimurium, but that it can efficiently compensate for zinc deficiency in a znuABC deleted strain. In fact, cobalt, but not cobalamin supplementation, rescued all major phenotypic defects of the znuABC strain, including the reduced ability to grow and swim in zinc-deficient media and the high susceptibility to hydrogen peroxide stress. Growth in a cobalt-supplemented defined medium led to the accumulation of large amounts of cobalt both in the wild type and in the znuABC strain. These data suggest that atoms of cobalt may be incorporated in bacterial proteins in place of zinc, ensuring their functionality. In support of this hypothesis we have shown that, in vivo, cobalt can accumulate in ribosomes and replace zinc in a periplasmic Cu,Zn superoxide dismutase (SodCII). Finally, we provide evidence of the ability of cobalt to modulate the intracellular concentration of zinc-regulated proteins (ZnuA, ZinT, and SodCII). see more Although some observations suggest that in some proteins the replacement of zinc with cobalt can lead to subtle structural changes, the data reported in this study indicate that Salmonella has the ability to use cobalt instead of zinc, without evident harmful effects for cell physiology.Magnetic composite materials i.e. elastomers, polymer gels, or polymer solutions with embedded magnetic nanoparticles are useful for many technical and bio-medical applications. However, the microscopic details of the coupling mechanisms between the magnetic properties of the particles and the mechanical properties of the (visco)elastic polymer matrix remain unresolved. Here we study the response of a single-domain spherical magnetic nanoparticle that is suspended in a polymer solution to alternating magnetic fields. As interactions we consider only excluded volume interactions with the polymers and hydrodynamic interactions mediated through the solvent. The AC susceptibility spectra are calculated using a linear response Green-Kubo approach, and the influences of changing polymer concentration and polymer length are investigated. Our data is compared to recent measurements of the AC susceptibility for a typical magnetic composite system [Roeben et al., Colloid Polym. Sci., 2014, 2013-2023], and demonstrates the importance of hydrodynamic coupling in such systems.The competition between excited state deactivation processes in mono and double-arm push-pull systems bearing pyridine, furan or thiophene (electron donors) and nitro groups (electron acceptors) was investigated in several solvents through nanosecond and femtosecond transient absorption spectroscopy. Triplet population is the main deactivation pathway for the mono-arm compounds. The large triplet production is mainly ascribed to 3(n,π*) states almost isoenergetic to S1, introduced by nitro groups, as predicted by TD-DFT calculations. The large triplet population may indeed be exploited to produce long-lived excitons for photovoltaic and optoelectronic applications. Two-arm furan and thiophene derivatives instead undergo strong ultrafast intramolecular charge transfer (ICT), which is responsible for their appreciable two-photon absorption cross-sections. In this case, significant fluorescence and singlet oxygen quantum yields are obtained, making these two compounds interesting as potential traceable photosensitizers in photodynamic therapy.Hexaammineruthenium(iii), RuHex3+, is a DNA-binding metal complex that is widely used as a redox marker for the indirect determination of DNA coverage at the electrode surface. The conversion of electrochemically quantifiable surface excess of RuHex3+ into DNA surface coverage requires the knowledge of the binding site size of RuHex3+ (s). Traditionally, s on surface-immobilized DNA has been assumed to be equivalent to that on solution-phase DNA, which was experimentally determined in previous studies. Nevertheless, the different local microenvironments existing inside DNA monolayers in comparison to that in bulk solutions cast doubt on the validity of this assumption. In this report, we used electrochemical techniques to investigate s on surface-immobilized DNA. The values of s inside the DNA monolayers were found to be significantly smaller than that reported on solution-phase DNA. Besides, s was found to depend on the DNA packing density and became larger by increasing the DNA surface coverage or hybridizing the surface-tethered DNAs with complementary strands. Our data indicate that the RuHex3+ method, in which an s value of 3 nucleotides is used for the conversion of RuHex3+ to DNA surface coverage, does not always give reliable results.Cu single atoms embedded in the C3N4 (Cu-SAs/C3N4) matrix exhibited high activity with 95% oxygenate selectivity for the direct conversion of methane at ambient temperature. The presence of abundant anchoring sites in C3N4 led to highly dispersed Cu-N4 moieties, which were suggested to be the underlying active sites for methane conversion.Elemental boron clusters do not form linear chain or monocyclic ring structures, which is in contrast to carbon. Based on computer global searches and quantum chemical calculations, we report on the viability of a curved boron single chain in binary Be6B7- cluster. The boron motif assumes a bow shape, being anchored on a Be6 prism. Such a motif, which appears to be highly strained in its free-standing form, is exotic in boron-based clusters and nanostructures. Chemically, the cluster is analogous to a "clam-and-pearl-chain" system at the nanoscale (about 1 nm in size), in which a Be6 clam moderately opens its mouth, except that a B7 pearl chain is too large to be encapsulated inside. The picture differs from a three-layered sandwich. This cluster features a hybrid Be2B7 monocyclic ring, which is octagonal in nature and supports double 10π/6σ aromaticity. The number of π bonds substantially surpasses that in bare boron clusters of similar sizes. Two Be3 rings in the prism are also σ aromatic, albeit with effective 1σ/1σ electron-counting only.