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er trials.OBJECTIVE Ovarian cancer (OC) is a deathful malignant tumor in women worldwide, and its poor prognosis mainly results from metastasis. Recently, microRNA (miRNA/miR) has been found to exert crucial functions in the progression of multiple tumors by affecting expressions of their targets. However, the biological roles and the potential mechanism of miR-489 in OC need further elucidation. BMS-354825 supplier PATIENTS AND METHODS Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was utilized to confirm the miR-489 expressions in OC tissue samples and cell lines. The functions of miR-489 were analyzed by performing functional assays, such as MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays. The downstream target of miR-489 was confirmed by TargetScan and luciferase reporter assay. Western blot was conducted to detect the expression of indicators associated with the down-stream signaling pathway. RESULTS MiR-489 was prominently downregulated in OC tissues and cells, and the decreased miR-489 expression was related to malignant clinicopathologic features and poor prognosis of OC patients. Functional assays demonstrated that miR-489 could suppress OC cell viability, invasion, and migration. X-linked inhibitor of apoptosis protein (XIAP) was identified as a target of miR-489 and partially regulated the functions of miR-489 in OC. Moreover, we found that miR-489 inhibits OC progression via regulating phosphatidyl-inositol 3-kinase/protein kinase B pathway (PI3K/AKT) and epithelial-to-mesenchymal transition (EMT). CONCLUSIONS Our results demonstrated that miR-489 inhibited OC development by directly binding to XIAP and regulating PI3K/Akt and EMT signal pathways, and miR-489 might serve as a promising biomarker for OC treatment in the future.OBJECTIVE To systemically evaluate the factors influencing the prognosis of osteosarcoma. MATERIALS AND METHODS Case-control studies (sample size>100) investigating the factors influencing the prognosis of osteosarcoma published from 1st January 1980 to 1st February 2019 were searched in the databases, including PubMed, Embase, and CBM. The meta-analysis was conducted within the Review Manager 5.3 software. RESULTS 22 studies were included. The 5-year overall survival (OS) of male patients was significantly lower than that of female patients (OR=0.84, 95% CI=0.76-0.93). There was no significant statistical difference in 5-year OS between the adolescent group (≤14 years old) and the adult group (>14 years old) (OR=0.88, 95% CI=0.68-1.14). Before standardized chemotherapy, which was started in 2000, the 5-year OS of patients receiving surgery and chemotherapy was significantly higher than patients only receiving surgery (OR=3.20, 95% CI=2.30-4.46). After 2000, the 5-year OS of patients receiving standardized chemotherapy was significantly higher than those undergoing non-standardized chemotherapy (OR=2.17, 95% CI=1.77-2.67). The 5-year OS of the limb-salvage surgery group was higher than that of the amputation surgery group (OR=2.17, 95% CI=1.77-2.67). The 5-year OS of patients with a good response to chemotherapy (Huvos III+IV) was higher than that of patients with poor response to chemotherapy (Huvos I+II) (OR=2.45, 95% CI=2.10-2.87). Patients without bone metastasis had significantly better 5-year OS than those with bone metastasis at initial diagnosis (OR=0.2, 95% CI=0.11-0.39). CONCLUSIONS The prognosis of male osteosarcoma patients was slightly worse than that of female patients. Surgery plus standardized chemotherapy can improve the 5-year OS of osteosarcoma patients. Patients who had undergone limb-salvage surgery had a better prognosis. Poor response to chemotherapy and bone metastasis had a negative influence on the prognosis of osteosarcoma.OBJECTIVE To study the roles and underlying mechanisms of long non-coding ribonucleic acid (lncRNA) H19 in the synovial cell proliferation and apoptosis in rats with rheumatoid arthritis (RA). MATERIALS AND METHODS A total of 30 Sprague-Dawley rats were randomly divided into Control group and Model group. The rat model of RA was induced by using type II collagen in Model group. The primary synovial cells were isolated from the synovial tissues of the rats and were assigned into Control group, Model group, and lncRNA H19 inhibitor intervention group. 5-Ethynyl-2'-deoxyuridine (EdU) staining was applied to detect cell proliferation in each group. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was employed to determine the cell apoptosis in each group. Western blotting assay was adopted to measure the expression levels of Notch1 and hairy/enhancer of split-1 (Hes1) in each group of cells. RESULTS The RA score of the Model group was higher than that of the Control group. Compared to the Control group, the expression of lncRNA H19, Notch, and Hes1 of the synovial cells in the Model group were significantly elevated. Besides, the cell proliferation rate of the Model was also increased, while the cell apoptosis rate was decreased compared with those in the Control group. Moreover, in comparison with Model group, lncRNA H19 inhibitor intervention group exhibited a lowered lncRNA H19 level, remarkably reduced cell proliferation rate and protein levels of Notch1 and Hes1, as well as notably raised cell apoptosis rate. CONCLUSIONS Our results indicated that lncRNA H19 inhibitor could repress the proliferation and promote the apoptosis of synovial cells in RA rats, which might be attributed to the inhibition of the Notch signaling pathway.OBJECTIVE This study aims to uncover the function of long non-coding RNA (lncRNA) HAGLR in the healing process of femoral neck fracture and the underlying mechanism. PATIENTS AND METHODS Expression levels of HAGLR, microRNA-19a-3p (miRNA-19a-3p) and TGFBR2 in fractured femoral neck tissues and adjacent normal tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Regulatory effects of HAGLR on viability, apoptosis, migration, and protein levels of BALP and Osteocalcin in MC3T3-E1 cells were determined. Dual-Luciferase reporter gene assay was conducted to assess the binding in HAGLR/miRNA-19a-3p/TGFBR2. In addition, relative levels of TGFBR2, p-smad2, p-smad3, and RUNX2 in MSCs influenced by HAGLR were detected. RESULTS HAGLR was downregulated in fractured femoral neck tissues. Knockdown of HAGLR reduced viability and migration, enhanced apoptotic rate, as well as downregulated BALP and Osteocalcin in MC3T3-E1 cells. HAGLR served as miRNA-19a-3p sponge, and miRNA-19a-3p directly targeted 3'-untranslated region (3'-UTR) of TGFBR2.