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Melting of snow and glaciers from the high-altitude Himalayan region is a significant water source to the major Himalayan rivers, especially in the upper Indus Basin (UIB), which contributes up to 70% of river discharge. Considering Indus Basin as a largest irrigation system dependent on snow and glacier melt runoff, it is imperative to study the rivers' current status and water budget. In this study we have performed a tracer-based hydrograph separation to quantify the contribution of seasonal snow, glacier melt, and groundwater to the Chandra River draining from a semi-arid region of the upper Indus basin, western Himalaya. Our study revealed a negligible control of summer (May-September 2017) precipitation and significant control of summer air temperature (May-September 2017) and winter precipitation over the Chandra River discharge, with 1 °C rise in air temperature leading to 22 m3s-1 (15% of mean) increase in the river discharge (R2 = 0.85; n = 541; p less then 0.001). The hydrograph separation of the Chandra River suggests groundwater (38.3 ± 5.6%; 96.8 m3s-1) as a significant source to the river runoff, followed by a direct contribution from glacier melt (30.9 ± 9%; 88.2 m3s-1) and seasonal snowmelt (30.6 ± 5.7%; 84.2 m3s-1), respectively, with negligible contribution from rainfall. Although groundwater is a significant contributor to the river runoff, the infiltration of seasonal snowmelt (54%) and glacier melt (46%) mostly contributed to the groundwater recharge. Present study establishes a linkage between seasonal snowmelt, glacier melt, groundwater, and the river runoff and would be useful to better model and predicts the future changes in the water resources of the upper Indus Basin.Adoption of renewable energy sources such as biomass has been increasing worldwide. In this study, fast pyrolysis as an acceptable and viable method to get renewable bio-oil and biochar is used. Different temperatures and N2 flow velocities were used in the fast pyrolysis process to evaluate the pyrolysis yield of biochar and bio-oil. The waste wood and pig manure were utilized to prepare biochar and bio-oil. X-ray fluorescence, X-ray diffraction, high-pressure liquid chromatograph, Micro confocal laser Raman spectrometer, Fourier transform infrared spectrometer, and dynamic shear rheometer were used to measure the chemical compositions, structure, and pyrolysis yield of biochar and bio-oil. The obtained results indicate that pyrolysis temperature increases the purity of inorganic oxide in biochar and N2 flow velocity promotes the yield of carbon in biochar. The increase of N2 flow velocity would increase the acid property of bio-oil and damage the products yield of bio-oil. It was also observed that biochar could remarkably alter the fundamental performances of petroleum asphalt including penetration, softening point, ductility, viscosity, and complex modulus. The most important is that the upgraded bio-oil can be used to replace partly or fully the petroleum asphalt which is a promising biomass application.Although lake water can be used as a source of drinking water and recreational activities, there is a dearth of research on the occurrence and fate of enteric viruses. Over a period of 14 months at six points in 2014-2015, we conducted monthly monitoring of the virological water quality of a Japanese lake. The lake receives effluent from three surrounding wastewater treatment plants and retains water for about two weeks. These features allowed us to investigate the occurrence and fate of viruses in the lake environment. Human enteric viruses such as noroviruses and their indicators (pepper mild mottle virus and F-specific RNA bacteriophage [FRNAPH] genogroups) were quantified by PCR-based assays. Additionally, FRNAPH genogroups were quantified by infectivity-based assays to estimate the degree of virus inactivation. Pepper mild mottle virus, genogroup II (GII) norovirus, and GI-FRNAPH were identified in relatively high frequencies (positive in >40% out of 64 samples), with concentrations ranging from 1.3 × 101 to 2.9 × 104 copies/L. Human enteric viruses and some indicators were not detected and less prevalent, respectively, after April 2015. Principal component analysis revealed that the virological water quality changed gradually over time, but its differences between the sampling points were not apparent. FRNAPH genogroups were inactivated during the warm season (averaged water temperature of >20 °C) compared to the cool season (averaged water temperature of less then 20 °C), which may have been due to the more severe environmental stresses such as sunlight and water temperature. This suggests that the infection risk associated with the use of the lake water may have been overestimated by the gene quantification assay during the warm season.The role of mangroves in sequestering metal and nutrients in sediment has been described in the past, but knowledge gaps still exist on storage capacity and recycling fluxes of elements in plant biomass, notably concerning their magnitude in root uptake and loss by litterfall. This study addresses the storage and transport pathways of 16 elements, classified as macro-nutrients (Ca, Mg, Na, K), micro-nutrients (Fe, Mn, Ni, Co, Cu, Cr, Zn, Mo), and potential toxicants (Al, Cd, Sn, Pb) in the world's largest mangroves, the Sundarbans. Elemental concentrations in plant organs were generally lower than in the sediment. The stock of macro and micro-nutrients in plant biomass varied from 60 to 2717 and 0.003 to 37.7 Mg ha-1 respectively, with highest values observed for Na and lowest for Cd. The Avicennia species exhibited the maximal accumulation of all elements. Translocation of major elements to different plant organs increased with increasing their concentrations in the sediment. Elemental loss via litterfall indicated that Sundarbans mangrove could act as a source, particularly of Mn, to the Bay of Bengal. Avexitide Moreover, belowground uptake of the 16 elements showed 2-3 fold higher fluxes than their loss via litterfall. There was a significant retention of some trace elements (notably Mo, Cd, and Sn) in plant biomass, which might allow one to use these mangroves for phytoremediation and restoration purposes. We conclude that mangroves efficiently store and remobilize major and trace elements from the sediments by root uptake and recycle back to sediment surface via litterfall.

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