Lindgrenhassing5253

Herein, polyaniline (PANI) with tuning morphology was in-situ synthesized within bacterial cellulose (BC)/silver nanoparticles hydrogels (AgNPs) that were prepared by green hydrothermal reduction method in different molarity of 0.01 and 0.25 of HCl solution along with the presence of polyethylene glycol (PEG). The synthesis of PANI in the presence of PEG in 0.01 M HCl led to the formation of rose-like morphology within nanocomposite aerogels with a size of 1.5-5.2 μm. All aerogels had the porosity and shrinkage of higher than 80% and lower than 10%, respectively. Rheology results showed a higher value of storage modulus (G') than that of loss modulus (G″) for all samples over the whole frequency regime. It confirmed by the loss factor (tan δ) value of less than 1 for all hydrogel samples. The synthesis of PANI within BC/Ag in 0.25 M of HCl solution resulted in a substantial rise of G' to nearly 1.5 × 104 Pa that was one order of magnitude higher than that of other hydrogels. However, the synthesis condition of PANI did not influence the antibacterial activity. In spite of unfavorable cell attachment onto nanocomposite aerogels, the cell proliferation increased steadily over the whole period of incubation. Octenyl succinic anhydride (OSA) modified gum arabic (GA) was synthesized and used as an emulsifier to stabilize cinnamon essential oil (CEO) emulsions. The structure and properties of chitosan based polyelectrolyte films incorporated with above OSA-GA stabilized CEO emulsions were investigated. Results showed that OSA modification introduced the hydrophobic groups, which greatly influenced the emulsification capability of GA. The antimicrobial activities of CEO emulsions were significantly enhanced by the synergistic effect of GA modification and ultrasonic treatment. When the proportions of CEO emulsion increased, the improved water barrier properties but deteriorated tensile properties of films were observed. The retention of CEO during storage was prolonged to 20 days and the release of CEO in food simulant was effectively inhibited as emulsion ratios increased to 20%, indicating the effectiveness of the system for CEO delivery. As a result, the antimicrobial activities of films significantly enhanced with the innovative incorporation of OSA-GA stabilized CEO emulsions. Precise prediction and measurement of dispersibility of silver nanoparticles (AgNPs) under atmospheric conditions are extremely vital for their potential commercial application. In the present work, the dispersibility of AgNPs capped by polysaccharide-protein from viscera of abalone (PSP-AgNPs) was studied using the combination of ultraviolet-visible spectroscopy (UV-vis), dynamic light scattering (DLS) and multiple-light-scattering (MLS) techniques. The results showed that the combination of UV/vis, DLS and MLS not only accurately determined the dispersibility of PSP-AgNPs, but also provided detailed information about the aggregation behavior of PSP-AgNPs. Furthermore, the results revealed a high dispersibility of PSP-AgNPs in the studied environment. The system temperature, pH value and thermal treatment (pasteurization and sterilization) had no effect on the dispersion of PSP-AgNPs in the effective concentration range against the pathogenic bacteria. Besides, an excellent stable dispersion and antibacterial activity against common pathogenic vibrio was also found in the dispersed PSP-AgNPs in seawater. Overall, the study provides a suitable method for the precise measurement of the dispersibility of AgNPs in environment. The AgNPs act as a potential bactericide with good dispersion and antibacterial activity in mariculture and other fields. The pharmaceutical application of artemether (ARM) as an anticancer natural agent is hampered due to its poor solubility and bioavailability. AZD7762 in vivo In the present study, ARM was encapsulated in human serum albumin nanoparticles (HSA NPs) via desolvation method led to improvement of the water solubility by 50 folds. In further, folate-decorated ARM-HSA NPs (F-ARM-HSA NPs) were developed to enhance targeted delivery to folate receptor alpha (FRα)-overexpressing breast cancer cells. The hydrodynamic diameter and the zeta potential value of F-ARM-HSA NPs were 198 ± 11.22 nm and -23 ± 0.88 mV, respectively. Fluorescent microscopy demonstrated an enhanced cellular uptake of F-ARM-HSA NPs by high FRα-expressing MDA-MB-231 breast cancer cells compared to low FRα-expressing SK-BR-3 breast cancer cells. Cytotoxicity assay revealed a small significant difference between cytotoxicity effect of targeted and non-targeted NPs in SK-BR-3 cells. However, in MDA-MB-231 cells due to FRα-mediated endocytosis, the F-conjugated NPs had less inhibitory concentration (IC50) value (19.82 μg/mL) and higher cytotoxicity after 72 h compared to non-targeted ARM-HSA NPs. Flow cytometry analysis indicated a more potent drug-induced apoptosis rather than necrosis. The results suggest that our novel F-ARM-HSA NPs are likely to be recommended as a promising candidate for combination therapy of FRα-overexpressing breast cancer cells. The major complications associated with the administration of ocular dosage forms are the precorneal loss due to the nasolacrimal drainage and high turnover of tear fluid. In situ forming gels are developed to overcome these complications. In situ gelling systems are polymeric solutions converted into a viscoelastic gel in the ocular surface due to a change in temperature, ionic strength, and pH. Recently, the use of responsive polysaccharides containing in situ gel formulation has increased due to its biocompatible nature, non-toxicity, and biodegradability. The research on in situ gel using polysaccharide in the delivery of drug molecules through the ocular route has diversified in various fields. However, there is no report are available that summaries this progress. The aim of this article is to bridge that lacuna. We discuss different polysaccharides based in situ gel including pH, temperature, ion and multiple sensitive used in ocular drug delivery. The nanoformulation incorporated polysaccharide in situ gels for ocular drug delivery has also been highlighted.