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In regions highly dependent on fossil fuels imports, biomethane represents a promising biofuel for the transition to a bio-based circular economy. While biomethane is typically produced via anaerobic digestion and upgrading, biomethanation of the synthesis gas (syngas) derived from the gasification of recalcitrant solid waste has emerged as a promising alternative. This work presents a comprehensive and in-depth analysis of the state-of-the-art and most recent advances in the field, compiling the potential of this technology along with the bottlenecks requiring further research. The key design and operational parameters governing syngas production and biomethanation (e.g. organic feedstock, gasifier design, microbiology, bioreactor configuration, etc.) are critically analysed.Bioelectrochemical systems (BES) have the potential to be used in a variety of applications such as waste biorefinery, pollutants removal, CO2 capture, and the electrosynthesis of clean and renewable biofuels or byproducts, among others. In contrast, many technical challenges need to be addressed before BES can be scaled up and put into real-world applications. Utilizing BES, this review article presents a state-of-the-art overall view of crucial concepts and the most recent innovative results and achievements acquired from the BES system. Special attention is placed on a hybrid approach for product recovery and wastewater treatment. There is also a comprehensive overview of waste biorefinery designs that are included. In conclusion, the significant obstacles and technical concerns found throughout the BES studies are discussed, and suggestions and future requirements for the virtual usage of the BES concept in actual waste treatment are outlined.Anaerobic ammonium oxidation (ANAMMOX) is a promising technology for sewage treatment. Alternating aerobic/anoxic conditions have been widely adopted to achieve partial nitrification (PN), so as to provide substrates for ANAMMOX. In this study, the feasibility of PN with the strategy of intermittent aeration was investigated under mainstream conditions. At a low dissolved oxygen (DO) concentration, the nitrogen conversion characteristic under different intermittent aeration modes was evaluated by mathematical simulation and experimental method with (1) ordinary activated sludge, (2) mixed sludge with anaerobic ammonia-oxidizing bacteria (AnAOB), and (3) PN sludge, as seed sludge. The existence of functional microorganisms, such as AnAOB and denitrifying bacteria, which can utilize nitrites, was the prerequisite for NOB activity inhibition in the alternating aerobic/anoxic condition. Therefore, low nitrite may be an important factor in NOB activity inhibition under alternating aerobic/anoxic conditions. This study demonstrated a key controlling factor for NOB activity inhibition with alternating aerobic/anoxic condition.Metals discharged from abandoned mines are a major source of pollution in many parts of the world. As a result, there is a growing need for suitable low-cost remediation methods. While a large literature base exists demonstrating the efficacy of biochar to remove metals from solution, most studies are confined to the laboratory. This study examines the effects on the biochar quality when scaling up production from laboratory to pilot scale. Pilot scale biochars were produced using a 600 kg batch pyrolysis reactor, these chars were then deployed in the field using a series of 100 mm × 1200 mm cylindrical treatment cells installed at the point of discharge from an abandoned mine site. Most biochars produced at a pilot removed more zinc under laboratory conditions, however all of the biochars showed a reduced performance when tested in the field, this ranged from a 14% to an 85% reduction depending on the biochar.The effects of microalgae harvesting methods on microalgal biomass quality were evaluated using three species namely the freshwater green alga Chlorella vulgaris, marine red alga Porphyridium purpureum and marine diatom Phaeodactylum tricornutum. Harvesting efficiencies of polyacrylamide addition, alkaline addition, and centrifugation ranged from 85 to 95, 59-92 and 100%, respectively, across these species. Morphology of the harvested cells (i.e. compromised cell walls) was significantly impacted by alkaline pH-induced flocculation for all three species. Over 50% of C. vulgaris cells were compromised with alkaline pH compared to less then 10% with polyacrylamide and centrifugation. The metabolic profiles varied depending on harvesting methods. Species-specific decrease of certain metabolites was observed. These results suggest that the method of harvest can alter the metabolic profile of the biomass amongst the three harvesting methods, polyacrylamide addition showed higher harvesting efficiency with less compromised cells and higher retention of industry important biochemicals.The limited efficiency of nitrogen removal has traditionally hindered wide application of simultaneous nitrification and denitrification (SND) technology. Here, the nitrogen removal characteristics of a sequencing batch reactor were studied by adopting a strategy of a step-feeding mode, synergistic regional oxygen limitation, and a mixed carbon source. The changes of the microbial population succession and nitrogen metabolism functional genes were analyzed. This strategy provided a favorable level of dissolved oxygen and continuous carbon sources for driving the denitrification process. The total nitrogen removal efficiency and SND rate reached 92.60% and 96.49%, respectively, by regulating the ratio of sodium acetate to starch in the step feed to 51. This procedure increased the relative abundance of denitrifying functional genes and induced the growth of a variety of traditional denitrifying bacteria and aerobic denitrifying bacteria participating in the process of nitrogen removal. Overall, this work offers a new strategy for achieving efficient SND.Urbanization and pollution are the major issues of the current time own to the exhaustive consumption of fossil fuels which have a detrimental effect on the nation's economies and air quality due to greenhouse gas (GHG) emissions and shortage of energy reserves. Algae, an autotrophic organism provides a green substitute for energy as well as commercial products. Algal extracts become an efficient source for bioactive compounds having anti-microbial, anti-oxidative, anti-inflammatory, and anti-cancerous potential. Besides the conventional approach, residual biomass from any algal-based process might act as a renewable substrate for fermentation. Likewise, lignocellulosic biomass, algal biomass can also be processed for sugar recovery by different pre-treatment strategies like acid and alkali hydrolysis, microwave, ionic liquid, and ammonia fiber explosion, etc. Residual algal biomass hydrolysate can be used as a feedstock to produce bioenergy (biohydrogen, biogas, methane) and biochemicals (organic acids, polyhydroxyalkanoates) via microbial fermentation.The recalcitrance of cellulosic biomass greatly hinders its enzymatic degradation. Expansins induce cell wall loosening and promote efficient cellulose utilization; however, the molecular mechanism underlying their action is not well understood. In this study, TlEXLX1, a fungal expansin from Talaromyces leycettanus JCM12802, was characterized in terms of phylogeny, synergy, structure, and mechanism of action. TlEXLX1 displayed varying degrees of synergism with commercial cellulase in the pretreatment of corn straw and filter paper. TlEXLX1 binds to cellulose via domain 2, mediated by CH-π interactions with residues Tyr291, Trp292, and Tyr327. Residues Asp237, Glu238, and Asp248 in domain 1 form hydrogen bonds with glucose units and break the inherent hydrogen bonding within the cellulose matrix. This study identified the expansin amino acid residues crucial for cellulose binding, and elucidated the structure and function of expansins in cell wall networks; this has potential applications in biomass utilization.

Surgical site infections (SSIs) are the most common and costly of all hospital-acquired infections, occurring in 5 percent of patients and accounting for 20% of all hospital-acquired infections. Preoperatively, we developed a protocol where patients were screened using hemoglobin A 1c (HbA1c) and nasal swabs. If HbA1c was greater than 9, patients were rescheduled for surgery when their HbA1c was less than 9. All patients then underwent nasal swabs to identify methicillin-sensitive Staphylococcus aureus/methicillin-resistant S.aureus in addition to standard chlorhexidine gluconate bathing. If positive, mupirocin ointment was used to treat the patients 5 days prior to surgery. We sought to measure the effectiveness of this protocol in reducing SSI in elective neurosurgical patients who were undergoing hardware implantation or had a procedure anticipated to last greater than 2 hours.

This was a retrospective review of patients undergoing elective neurosurgical procedures at Conemaugh Memorial Medical Center from 1/1/2014 to 06/30/2016. The intervention period was from 7/1/2016 to 12/20/2018, which included the patients undergoing the protocol.

The preintervention group consisted of 817 cases with a 2.7% infection rate (22 SSIs). find more The intervention group consisted of 822 cases with a 0.1% infection rate (1 SSI). This observed difference was statistically significant (P= 0.003).

This retrospective review of a presurgical protocol with measuring of HbA1c and nasal swabs revealed a significant decrease in the infection rate of patients undergoing elective neurosurgical procedures. Additional investigations are necessary; however, we recommend its use.

This retrospective review of a presurgical protocol with measuring of HbA1c and nasal swabs revealed a significant decrease in the infection rate of patients undergoing elective neurosurgical procedures. Additional investigations are necessary; however, we recommend its use.

We aimed to predict hematoma expansion in intracerebral hemorrhage (ICH) patients by using the deep learning technique.

We retrospectively collected data from ICH patients treated between May 2015 and May 2019. Head computed tomography (CT) scans were performed at admission, and 6 hours, 24 hours, and 72 hours after admission. CT scans were mandatory when neurologic deficits occurred. Univariate and multivariate analyses were conducted to illustrate the association between clinical variables and hematoma expansion. Convolutional neural network (CNN) was adopted to predict hematoma expansion based on brain CT slices. In addition, 5 machine learning methods, including support vector machine, multi-layer perceptron, naive Bayes, decision tree, and random forest, were also performed to predict hematoma expansion based on clinical variables for comparisons.

A total of 223 patients were included. It was revealed that patients' older age (odds ratio [95% confidence interval] 1.783 [1.417-1.924]), cerebral hemosed on clinical variables only. Deep learning technology could favorably predict hematoma expansion from non-contrast CT scan images.

Dietary triglycerides (TG) retained in the intestine after a meal can be mobilized many hours later by glucagon-like peptide-2 (GLP-2) in humans and animal models, despite the well-documented absence of expression of the GLP-2 receptor on enterocytes. In this study, we examined the site of GLP-2 action to mobilize intestinal lipids and enhance chylomicron production.

In mesenteric lymph duct-cannulated rats, we assessed GLP-2-stimulated lymph flow rate, TG concentration, TG output, and apoB48 abundance 5h after an intraduodenal lipid bolus, in the presence of a validated GLP-2 antagonist or vehicle. Additionally, the same GLP-2-stimulated parameters were examined in the presence or absence of cis-Golgi disruption by Brefeldin A (BFA).

Compared to placebo, GLP-2 administration increased lymph flow by 2.8-fold (P<0.001), cumulative lymph volume by 2.69-fold (P<0.001) and total TG output 2-fold (P=0.015). GLP-2 receptor antagonism markedly diminished GLP-2's ability to stimulate lymph flow, cumulative lymph volume and total TG output, demonstrating the dependence of GLP-2 stimulation of lymph flow and TG output on its receptor activation.