Lemminganker5856

WRKY transcription factors (TFs) are a large plant-specific family of TFs that govern development and biotic/abiotic stress responses in plants. We have identified SlWRKY23 as a gene primarily expressed in roots. SlWRKY23 encodes a protein of 320 amino acids that functions as a transcriptional activator. It is transcriptionally up-regulated by ethylene, BAP and salicylic acid treatment but suppressed by IAA. Expression of SlWRKY23 in transgenic Arabidopsis affects sensitivity of roots to ethylene, JA and auxin with transgenic plants showing hypersensitivity to ethylene, JA and auxin-mediated primary root growth inhibition. This hypersensitivity is correlated with higher expression of ERF1 and ARF5 that mediate responses to these hormones. SlWRKY23 expression also affects aerial growth with transgenic plants showing greater number of leaves but smaller rosettes. Flowering time is reduced in transgenic lines and these plants also show a greater number of inflorescence branches, siliques and seeds. The siliques are longer and compactly packed with seeds but seeds are smaller in size. Root biomass shows a 25% decrease in transgenic SlWRKY23 Arabidopsis plants at harvest compared with controls. The studies show that SlWRKY23 regulates plant growth possibly through modulation of genes controlling hormone responses.Drought stress is one of the most prevalent environmental factors limiting faba bean (Vicia faba L.) crop productivity. β-aminobutyric acid (BABA) is a non-protein amino acid that may be involved in the regulation of plant adaptation to drought stress. The effect of exogenous BABA application on physiological, biochemical and molecular responses of faba bean plants grown under 18% PEG-induced drought stress were investigated. The results showed that the application of 1 mM of BABA improved the drought tolerance of faba bean. The application of BABA increased the leaf relative water content, leaf photosynthesis rate (A), transpiration rate (E), and stomatal conductance (gs), thereby decreased the water use efficiency. Furthermore, exogenous application of BABA decreased production of hydrogen peroxide (H2O2), malondialdehyde and electrolyte leakage levels, leading to less cell membrane damage due to oxidative stress. Regarding osmoprotectants, BABA application enhanced the accumulation of proline, and soluble sugars, which could improve the osmotic adjustment ability of faba bean under drought challenge. Interestingly, mended antioxidant enzyme activities like catalase, guaiacol peroxidase, ascorbate peroxidase and superoxide dismutase and their transcript levels may lead to counteract the damaging effects of oxidative stress and reducing the accumulation of harmful substances in BABA-treated faba bean plants. In addition, exogenous BABA significantly induced the accumulation of drought tolerance-related genes like VfMYB, VfDHN, VfLEA, VfERF, VfNCED, VfWRKY, VfHSP and VfNAC in leaves and roots, suggesting that BABA might act as a signal molecule to regulate the expression of drought tolerance-related genes.Algal supplements can improve crop productivity and afford protection against abiotic stress by virtue of their rich content of plant nutrients and bioactive compounds. The present work investigates the relative efficiency of the biomass and extract of the brown alga Dictyota dichotoma in protection of rice against salinity and water stress. Rice (Oryza sativa L.) cv. Sakha 101 was grown on a silty clay soil amended with the aqueous extract and powder of D. dichotoma under NaCl and PEG 6000 stress at water potential of - 0.492 MPa. Abiotic stress, particularly water stress, reduced rice growth and concentrations of K+ and protein but increased soluble sugars, starch, proline and Na+ concentrations of plant tissues, with counterbalancing effect of algal amendment. The benefit of algal amendment was greater for algal extract than algal powder and under water stress than salt stress. Algal amendment and abiotic stress promoted catalase and peroxidase activities in rice leaves with variable effect on polyphenol oxidase. The benefit of D. dichotoma to rice can be related to macro- and micro-nutrients, growth hormones, phenolics, flavonoids, sterols, vitamins and fucoidan. Cyclopamine datasheet The production of toxic intermediates as a result of fermentation of the algal biomass in the paddy soil might reduce the benefit of algal amendment. Although rice is salt-sensitive, it is more resistant to salt stress than to drought stress. The ability of rice to retain Na+ in the root is pivotal for stress resistance, but the role of K+ partitioning is less evident.Fungicides are widely used for controlling fungi in crop plants. However, their roles in conferring abiotic stress tolerance are still elusive. In this study, the effect of tebuconazole (TEB) and trifloxystrobin (TRI) on wheat seedlings (Triticum aestivum L. cv. Norin 61) was investigated under salt stress. Seedlings were pre-treated for 48 h with fungicide (1.375 µM TEB + 0.5 µM TRI) and then subjected to salt stress (250 mM NaCl) for 5 days. Salt treatment alone resulted in oxidative damage and increased lipid peroxidation as evident by higher malondialdehyde (MDA) and hydrogen peroxide (H2O2) content. Salt stress also decreased the chlorophyll and relative water content and increased the proline (Pro) content. Furthermore, salt stress increased the dehydroascorbate (DHA) and glutathione disulfide (GSSG) content while ascorbate (AsA), the AsA/DHA ratio, reduced glutathione (GSH) and the GSH/GSSG ratio decreased. However, a combined application of TEB and TRI significantly alleviated growth inhibition, photosynthetic pigments and leaf water status improved under salt stress. Application of TEB and TRI also decreased MDA, electrolyte leakage, and H2O2 content by modulating the contents of AsA and GSH, and enzymatic antioxidant activities. In addition, TEB and TRI regulated K+/Na+ homeostasis by improving the K+/Na+ ratio under salt stress. These results suggested that exogenous application of TEB and TRI rendered the wheat seedling more tolerant to salinity stress by controlling ROS and methylglyoxal (MG) production through the regulation of the antioxidant defense and MG detoxification systems.